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Volume 14, Issue 1 (January 2012) 14, 88–93; 10.1038/aja.2011.57

Testicular biopsy: clinical practice and interpretation

Gert R Dohle1, Saad Elzanaty2 and Niels J van Casteren1

1 Department of Urology, Erasmus MC, 3000 CA Rotterdam, The Netherlands
2 Department of General Surgery, Urology Section, Kristianstad Central Hospital, Kristianstad SE 291 85, Sweden

Correspondence: Dr GR Dohle, (g.r.dohle@erasmusmc.nl)

Received 30 May 2011; Revised 5 September 2011; Accepted 5 September 2011; Published online 12 December 2011


Testicular biopsy was considered the cornerstone of male infertility diagnosis for many years in men with unexplained infertility and azoospermia. Recent guidelines for male infertility have limited the indications for a diagnostic testicular biopsy to the confirmation of obstructive azoospermia in men with normal size testes and normal reproductive hormones. Nowadays, testicular biopsies are mainly performed for sperm harvesting in men with non-obstructive azoospermia, to be used for intracytoplasmic sperm injection. Testicular biopsy is also performed in men with risk factors for testicular malignancy. In a subgroup of infertile men, there is an increased risk for carcinoma in situ of the testis, especially in men with a history of cryptorchidism and testicular malignancy and in men with testicular atrophy. Ultrasonographic abnormalities, such as testicular microlithiasis, inhomogeneous parenchyma and lesions of the testes, further increase the risk of carcinoma in situ (CIS) in these men. For an accurate histological classification, proper tissue handling, fixation, preparation of the specimen and evaluation are needed. A standardized approach to testicular biopsy is recommended. In addition, approaches to the detection of CIS of the testis testicular immunohistochemistry are mandatory. In this mini-review, we describe the current indications for testicular biopsies in the diagnosis and management of male infertility.

Keywords: carcinoma in situ of the testis; diagnosis; male infertility; testicular sperm extraction; testicular biopsy; testicular germ cell malignancies

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.