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Abstract

Volume 15, Issue 5 (September 2013) 15, 692–696; 10.1038/aja.2013.32

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

Jun-Wei Gai1,2,*, Wasilijiang Wahafu3,*, Hui Guo4, Miao Liu5, Xu-Chang Wang1,2, Yun-Xiang Xiao1,2, Liang Zhang6, Zhong-Cheng Xin2,7 and Jie Jin1,2

1Department of Urology, Peking University First Hospital, Beijing 100034, China
2The Institute of Urology, Peking University, Beijing 100034, China
3Department of Urology, PLA General Hospital, Beijing 100853, China
4Department of Urology, Tsinghua University First Hospital, Beijing 100016, China
5Hedong Center for Disease Control and Prevention, Tianjin 300151, China
6Department of Orthopaedic Surgery, Beijing Jishuitan Hospital, Fourth Clinical College of Peking University, Beijing 100035, China
7Andrology Center, Peking University First Hospital, Beijing 100034, China

Correspondence: Professor ZC Xin, (xinzc@bjmu.edu.cn); Professor J Jin, (Jinjie@vip.163.com)

Received 18 November 2012; Revised 20 December 2012; Accepted 14 March 2013 Advance online publication 3 June 2013

Abstract

We investigated the expression of hydrogen sulphide (H2S) in human and rat lower urinary tract (including bladder, prostate and urethra) tissues, and we sought to determine whether H2S induces relaxation of human and Sprague–Dawley (SD) rat bladder strips. Human normal lower urinary tract tissue was obtained for the evaluation of endogenous H2S productivity using a sulphide-sensitive electrode and for the analysis of the expression levels of all three synthases of endogenous H2S, cystathionine β-synthase (CBS), cystathionine γ lyase (CSE) and 3-mercaptopyruvate sulphur transferase (MPST, as known as 3-MST) by Western blot assay. CBS, CSE and MPST were located in human sample slides by immunohistochemistry. Human and male adult SD rat bladder strips were tested for H2S function with a transducer and recorded. All experiments were repeated six times. The endogenous H2S productivity and the H2S synthases had various distributions in the human and rat lower urinary tract tissues and were located in both epithelial and stromal sections. L-cysteine (L-Cys, a substrate of CBS, CSE and MPST) elicited relaxation in a dose-dependent manner on human bladder strips pre-contracted by acetylcholine chloride. This effect could be diminished by the ATP-sensitive potassium ion (KATP) channel blocker glibenclamide (GLB), the CSE inhibitor DL-propargylglycine (PPG) and the CBS inhibitor hydroxylamine (HA). H2S and its three synthases were present in the human and rat lower urinary tract tissues and relaxed human and rat bladder strips, which implied that endogenous H2S might play a role in physiological function and pathological disorders of the lower urinary tract symptoms (LUTS) or overactive bladder (OAB).

Keywords: cystathionine β-synthase; cystathionine γ lyase; detrusor; hydrogen sulphide; lower urinary tract symptoms; 3-mercaptopyruvate sulphur transferase

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.