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Abstract

Volume 9, Issue 3 (May 2007) 9, 377–381; 10.1111/j.1745-7262.2007.00241.x

Assessment of heme oxygenase-1 (HO-1) activity in the cavernous tissues of sildenafil citrate-treated rats

M Talaat Abdel Aziz, M Farid Al-Asmar, Taymour Mostafa, Hazem Atta, Laila Rashed, Dina Sabry, Shedeed Ashour and Ahmed T Abdel Aziz

1.Molecular Biology Unit, Medical Biochemistry Department, Faculty of Medicine, Cairo University, Cairo 11553, Egypt
2.Medical Biochemistry Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
3.Andrology Department, Faculty of Medicine, Cairo University, Cairo 11553, Egypt

Correspondence: Prof. Taymour Mostafa, Andrology Department, Faculty of Medicine, Cairo University, Cairo 11553, Egypt. Fax: +20-2363-2297. E-mail: taymour1155@link.net

Received 6 May 2006; Accepted 22 September 2006.

Abstract

Aim: To assess heme oxygenase-1 (HO-1) activity in the cavernous tissue of sildenafil citrate-treated rats.

Methods: One hundred and ninety-two Sprague-Dawley male rats, divided into four equal groups, were investigated. Group 1, the control group, received regular animal chow; group 2 received sildenafil citrate by intragastric tube; group 3 received sildenafil and HO inhibitor (zinc protoporphyrin, ZnPP); and group 4 received sildenafil and nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester (L-NAME). Twelve rats from each group were killed after 0.5 h, 1 h, 2 h and 3 h of drug administration. Then HO-1 activity, cGMP levels and NOS enzymatic activity in the cavernous tissues were estimated.

Results: In cavernous tissue, HO-1 activity, NOS enzymatic activity and cGMP concentration increased significantly in sildenafil-treated rats compared to other groups throughout the experiment. Rats receiving either HO or NOS inhibitors showed a significant decrease in these parameters. HO-1 cavernous tissue activity and NOS enzymatic activity demonstrated a positive significant correlation with cGMP levels (r = 0.646, r = 0.612 respectively; P < 0.001).

Conclusion: The actions of PDE5 inhibitor sildenafil citrate in the cavernous tissue are partly mediated through the interdependent relationship between both HO-1 and NOS activities.

Keywords: erectile dysfunction, heme oxygenase, sildenafil citrate, nitric oxide synthase, carbon monoxide

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.