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Volume 10, Issue 4 (July 2008) 10, 667–674; 10.1111/j.1745-7262.2008.00414.x
Cytoprotective effects of Morinda officinalis against hydrogen peroxide-induced oxidative stress in Leydig TM3 cells
Mun-Seog Chang, Won-Nam Kim, Woong-Mo Yang, Hyu-Young Kim, Ji-Hoon Oh and Seong-Kyu Park
Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea
Correspondence: Seong-Kyu Park, M.D., Ph.D., Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea. Fax: +82-2-961-0536. E-mail: comskp@khu.ac.kr
Received 28 August 2007; Accepted 15 February 2008.
| Abstract |
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Aim: To investigate the antioxidant effects of Morinda officinalis (Morindae radix, MR) on H2O2-induced oxidative stress in cultured mouse TM3 Leydig cells.
Methods: We carried out 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells.
Results: MR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 μmol H2O2, and treatment of the cells with 250 μg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and CAT activity (74.6 units of CAT/mg protein, P < 0.001).
Conclusion: These findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H2O2-induced oxidative stress.
Keywords: Morindae radix, Leydig cell, testosterone, hydrogen peroxide, antioxidant
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