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Abstract

Volume 16, Issue 2 (March 2014) 16, 309–313; 10.4103/1008-682X.122583

Bactericidal/permeability-increasing protein originates in both the testis and the epididymis and localises in mouse spermatozoa

Zhong-Ping Zhou 1,2*, Xiao-Yu Xia 1,2*, Qiang-Su Guo 1,2, Chen Xu 1,2

1Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai; 2Shanghai Key Laboratory of Reproductive Medicine, Shanghai, China

Correspondence: Dr. C Xu

Abstract

Bactericidal/permeability–increasing protein (BPI) is an endogenous antibiotic protein with activity against gram–negative bacteria. In the present study, we examined the expression of BPI in postnatal mouse testes and epididymides as well as the subcellular localization within epididymal spermatozoa. Our results showed that, BPI mRNA was expressed in testis and epididymis independently. Throughout the epididymis, the BPI protein level gradually decreased in the epididymal epithelium in a spatial manner, specialized within the cytoplasm of clear cells in the cauda part. We detected BPI proteins in intact acrosome, implying its testicular origin; on the other hand, after the acrosome reaction, BPI proteins were observed dispersed across the entire sperm head, especially enriched at the equatorial segment. Our findings suggested a dual origin of the BPI that generated both in the testis and epididymis, and associated with mouse spermatozoa. BPI protein might be involved in the dynamics modification of the sperm plasma membrane and also the fertilization process.

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.