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Abstract

Volume 19, Issue 2 (March 2017) 19, 208–213; 10.4103/1008-682X.173442

Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples

Osamu Inoue1, Naoaki Kuji2, Hiroe Ito2, Mitsutoshi Yamada1, Toshio Hamatani1, Aimi Oyadomari3, Shingo Kato3, Hideji Hanabusa4, Keiichi Isaka2, Mamoru Tanaka1

1 Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 1608582, Japan
2 Department of Obstetrics and Gynecology, Tokyo Medical College, Tokyo 1600023, Japan
3 Department of Microbiology, Keio University School of Medicine, Tokyo 1608582, Japan
4 Department of Hematology, Ogikubo Hospital, Tokyo 1670035, Japan

Correspondence: Prof. N Kuji (naoaki@tokyo-med.ac.jp)

Date of Submission 17-May-2015 Date of Decision 31-Jul-2015 Date of Acceptance 07-Dec-2015 Date of Web Publication 23-Feb-2016

Abstract

Abstract
To evaluate the clinical efficacy of a procedure comprising a combination of Percoll continuous density gradient and modified swim-up techniques for the removal of human immunodeficiency virus type 1 (HIV-1) from the semen of HIV-1 infected males, a total of 129 couples with an HIV-1 positive male partner and an HIV-1 negative female partner (serodiscordant couples) who were treated at Keio University Hospital between January 2002 and April 2012 were examined. A total of 183 ejaculates from 129 HIV-1 infected males were processed. After swim-up, we successfully collected motile sperms at a recovery rate as high as 100.0% in cases of normozoospermia (126/126 ejaculates), oligozoospermia (6/6), and asthenozoospermia (36/36). The recovery rate of oligoasthenozoospermia was 86.7% (13/15). In processed semen only four ejaculates (4/181:2.2%) showed viral nucleotide sequences consistent with those in the blood of the infected males. After using these sperms, no horizontal infections of the female patients and no vertical infections of the newborns were observed. Furthermore, no obvious adverse effects were observed in the offspring. This protocol allowed us to collect HIV-1 negative motile sperms at a high rate, even in male factor cases. We concluded that our protocol is clinically effective both for decreasing HIV-1 infections and for yielding a healthy child.

Keywords: assisted reproductive technology; human immunodeficiency virus type 1; serodiscordant couple; viral nucleotide sequence

Keywords: assisted reproductive technology; human immunodeficiency virus type 1; serodiscordant couple; viral nucleotide sequence

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