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Abstract

Volume 18, Issue 4 (July 2016) 18, 600–606; DOI:10.4103/1008-682X.157650

Interaction between basigin and monocarboxylate transporter 2 in the mouse testes and spermatozoa

Cheng Chen, Mamiko Maekawa, Kenji Yamatoya, Masami Nozaki, Chizuru Ito, Toshihiko Iwanaga, Kiyotaka Toshimori

1 Department of Anatomy and Developmental Biology, Graduate School of Medicine, Chiba University, Chiba, Japan
2 Department of Cell Biology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
3 Laboratory of Histology and Cytology, Department of Anatomy, Graduate School of Medicine, Hokkaido University, Hokkaido, Japan

Correspondence: Dr. M Maekawa (mmaekawa@dpc.agu.ac.jp)

Received: 30 January 2015; Revised: 10 March 2015; Accepted: 06 May 2015

Abstract

Basigin is a member of the immunoglobulin superfamily and plays various important roles in biological events including spermatogenesis. To examine the basigin molecular variants during spermatogenesis and sperm maturation in the mouse, immunoprecipitated basigin samples from testis and epididymal spermatozoa were analyzed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). The results demonstrated that basigin molecules from the testis and spermatozoa were separable into two major bands and that the differences in the molecular sizes were possibly because of an endoproteolytic cleavage. Since basigin is known to be a chaperone for the monocarboxylate transporter 1 (MCT1), the localization of basigin, MCT1 and MCT2 was examined during postnatal testicular development. Immunohistochemical studies showed different expression patterns of MCT1 and MCT2. MCT1 was localized on the surface of spermatogonia, spermatocytes, and spermatids. In contrast, MCT2 appeared on the principal piece of spermatozoa in the testis, where basigin was also observed. In mature epididymal spermatozoa, MCT2 was located on the midpiece, where basigin co-localized with MCT2 but not with MCT1. Furthermore, MCT2 was immunoprecipitated with basigin in mouse testes and sperm. These results suggest that basigin has a functional role as a binding partner with MCT2 in testicular and epididymal spermatozoa.

Keywords: basigin; monocarboxylate transporter; sperm maturation; spermatogenesis; spermatozoa; testis

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.