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Abstract

Volume 19, Issue 2 (March 2017) 19, 203–207; DOI:10.4103/1008-682X.179155

Regeneration of rat corpora cavernosa tissue by transplantation of CD133 + cells derived from human bone marrow and placement of biodegradable gel sponge sheet

Shogo Inoue1, Katsutoshi Miyamoto1, Shunsuke Shinmei2, Koichi Shoji1, Jun Teishima1, Kazuhiro Sentani3, Wataru Yasui3, Akio Matsubara1

1 Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan
2 Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima; Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan
3 Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan

Correspondence: Dr. S Inoue (inosyogo@hiroshima-u.ac.jp)

Date of Submission 17-Jun-2015 Date of Decision 16-Sep-2015 Date of Acceptance 01-Mar-2016 Date of Web Publication 07-Jun-2016

Abstract

The objective is to develop an easier technique for regenerating corpora cavernosa tissue through transplantation of human bone marrow-derived CD133 + cells into a rat corpora cavernosa defect model. We excised 2 mm × 2 mm squares of the right corpora cavernosa of twenty-three 8-week-old male nude rats. Alginate gel sponge sheets supplemented with 1 × 10 4 CD133 + cells were then placed over the excised area of nine rats. Functional and histological evaluations were carried out 8 weeks later. The mean intracavernous pressure/mean arterial pressure ratio for the nine rats (0.34258 ± 0.0831) was significantly higher than that for eight rats with only the excision (0.0580 ± 0.0831, P = 0.0238) and similar to that for five rats for which the penis was exposed, and there was no excision (0.37228 ± 0.1051, P = 0.8266). Immunohistochemical analysis revealed that the nine fully treated rats had venous sinus-like structures and quantitative reverse transcription polymerase chain reaction analysis of extracts from their alginate gel sponge sheets revealed that the amounts of mRNA encoding the nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) were significantly higher than those for rats treated with alginate gel sheets without cell supplementation (NGF: P = 0.0309; VEGF: P < 0.0001). These findings show that transplantation of CD133 + cells accelerates functional and histological recovery in the corpora cavernosa defect model.

Keywords: alginate gel sponge sheet; CD133 + cell; corpora cavernosa

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