Home  |  Archive  |  Online Submission  |  News & Events  |  Subscribe  |  APFA  |  Society  |  Links  |  Contact Us  |  中文版
Search   
 
Journal

Ahead of print
Authors' Accepted
    Manuscripts
new!
Current Issue
Archive
Acknowledgments
Special Issues
Browse by Category

Manuscript Submission

Online Submission
Online Review
Instruction for Authors
Instruction for Reviewers
English Corner new!

About AJA

About AJA
Editorial Board
Contact Us
News

Resources & Services

Advertisement
Subscription
Email alert
Proceedings
Reprints

Download area

Copyright licence
EndNote style file
Manuscript word template
Guidance for AJA figures
    preparation (in English)

Guidance for AJA figures
    preparation (in Chinese)

Proof-reading for the
    authors

AJA Club (in English)
AJA Club (in Chinese)

Links

Meetings
Journals
Societies & Institutes
Hospitals
Databases & Libraries
Companies
Websites
Other links

 
Abstract

Volume 19, Issue 1 (January 2017) 19, 91–97; DOI:10.4103/1008-682X.182817

Therapeutic effects of adipose-derived stem cells-based microtissues on erectile dysfunction in streptozotocin-induced diabetic rats

Feng Zhou1, Yu Hui1, Hua Xin2, Yong-De Xu3, Hong-En Lei3, Bi-Cheng Yang3, Rui-Li Guan3, Meng Li4, Jian-Quan Hou5, Zhong-Cheng Xin3

1 Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034; Department of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China
2 Department of Ophthalmology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, China
3 Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China
4 Department of Urology, General Hospital of Ningxia Medical University, Ningxia Medical University, Ningxia 750021, China
5 Department of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China

Correspondence: Prof. ZC Xin (xinzc@bjmu.edu.cn) or Prof. JQ Hou (xf192@163.com)

Date of Submission 22-Dec-2015 Date of Decision 24-Feb-2016 Date of Acceptance 16-May-2016 Date of Web Publication 24-Jun-2016

Abstract

This study aimed to explore the therapeutic effects of adipose-derived stem cells (ADSCs)-based microtissues (MTs) on erectile dysfunction (ED) in streptozotocin (STZ)-induced diabetic rats. Fifty-six 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ (60 mg kg−1 ), and 8 weeks later, the determined diabetic rats randomly received intracavernous (IC) injection of phosphate buffer solution (PBS), ADSCs, or MTs. Another eight normal rats equally got IC injection of PBS. MTs were generated with a hanging drop method, and the injected cells were tracked in ADSC- and MT-injected rats. Four weeks after the treatments, intracavernous pressure (ICP), histopathological changes in corpus cavernosum (CC), and functional proteins were measured. Rat cytokine antibody array was used to detect ADSCs or MTs lysate. The results showed that MTs expressed vascular endothelial growth factor (VEGF), nerve growth factor (NGF), and tumor necrosis factor-stimulated gene-6 (TSG-6). MTs injection had a higher retention than ADSCs injection and MTs treatment improved ICP, neuronal nitric oxide synthase (nNOS) expression, smooth muscle, and endothelial contents in diabetic rats, ameliorated local inflammation in CC better. Thus, our findings demonstrate that IC injection of MTs improves erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.

Keywords: adipose-derived stem cells; diabetes; erectile dysfunction; microtissues; paracrine factors

Full Text | PDF |

 
Browse:  222
 
Copyright 1999-2017  Shanghai Materia Medica, Shanghai Jiao Tong University.  All rights reserved