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Online First

10.4103/aja.aja_4_21

Testicular germ cell tumors type 2 have high RNA expression of LDHB, the gene for lactate dehydrogenase subunit B

Finn Edler von Eyben1, Jorge Parraga-Alava2,3, Shi-Ming Tu4

1 Center for Tobacco Control Research, Birkevej 17, Odense M DK-5230, Denmark
2 Facultad de Ciencias Informáticas, Universidad Técnica de Manabí, Portoviejo 130105, Ecuador
3 Department of Informatics Engineering, Santiago University, Santiago 917020, Chile
4 Department of Urology, University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030, USA

Correspondence: Dr. FE von Eyben (finn113edler@mail.tele.dk)

09-Feb-2021

Abstract

This study analyzed RNA expression of genes for three serum tumor markers, alpha fetoprotein (AFP), human chorionic gonadotropin (hCG), and lactate dehydrogenase (LDH), in patients with testicular germ cell tumors (TGCT) type 2. The gene AFP encodes AFP, the gene for chorionic gonadotropin beta polypeptide 5 (CGB5) encodes a major part of the specific beta subunit of hCG, and the genes for LDH subunit A (LDHA), LDH subunit B (LDHB), and LDH subunit C (LDHC) encode three different subunits of LDH. LDHB encodes the LDHB subunit present as a tetramer in LDH isoenzyme 1 (LDH-1). We examined three datasets with 203 samples of normal testis tissue (NT) and TGCT type 2. Yolk sac tumor (YST) expressed RNA of AFP fourteen thousand times higher than seminoma (SE), embryonal carcinoma (EC), and teratoma (TER) combined (P = 0.00015). In the second microarray, choriocarcinoma (CC) expressed RNA of CGB5 ten times higher than other histologic types of TGCT combined. EC expressed RNA of LDHB twice higher than SE, YST and TER combined (P = 0.000041). EC expressed RNA of LDHB higher than that YST expressed RNA of AFP and that CC expressed RNA of CGB5. In conclusion, TGCT type 2 expressed RNA of LDHB markedly higher than the RNA of 23 other candidate genes for TGCT type 2.

Keywords: alpha fetoprotein; gene regulation; human chorionic gonadotropin; lactate dehydrogenase; lactate dehydrogenase isoenzymes; testicular germ cell tumors

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