Trevor Graham Cooper
Qualifications
B.Sc. (1970) First Class Honours in
Physiology & Biochemistry
1967-1970: Undergraduate in Dept.
Physiology & Biochemistry, University of Reading, UK
Ph.D. (1974) thesis "Steroid
transport in the mammalian testis"
1970-1973: Postgraduate research in
physiology of the testis, Dept. Physiology & Biochemistry, University of
Reading, U.K. (Supervisor Prof. G.M.H. Waites; External Examiner Prof. R.V.
Short, F.R.S)
C.Biol. (Chartered Biologist) (1983)
M.I.Biol. (Member of Institute of
Biology) (1983)
F.I.Biol. (Fellow of Institute of
Biology) (1989)
Academician EAA. (European Academy of
Andrology) (1992)
EurProBiol. (1996) (Registered as
European Biologist)
Employment
1974-1975 Postdoctoral Research Fellow,
Dept. Obstetrics & Gynecology, Vanderbilt University, Nashville, Tennessee,
U.S.A. (with Prof. M-C. Orgebin-Crist)
1976 Ford Foundation Fellow, Dept.
Anatomy, Harvard Medical School, Boston, Massachusetts, U.S.A. (with Prof. D.W.
Hamilton)
1977-1980 M.R.C. Postdoctoral Research
Assistant, Dept. Physiology & Biochemistry, University of Reading, U.K.
(with Prof. G.M.H. Waites)
1980-1983 Wellcome Trust Fellow in
Veterinary Medicine, Dept. Physiology, University of Bristol Medical School,
U.K. (with Dr. D.M. Woolley)
1983 U.N. Consultant, National Research
Institute for Family Planning, Beijing, China
1984-1985 M.R.C. Research Fellow, Dept.
Physiology, University of Bristol Medical School, U.K. (with Dr. D.M. Woolley)
1985-1987 Max Planck Research Fellow,
Max Planck Society Clinical Research Unit for Reproductive Medicine, Münster,
Germany (with Prof. E. Nieschlag)
1988-1993 Scientist, Institute of
Reproductive Medicine of the University, Münster, Germany (with Prof. E.
Nieschlag)
1993-present Scientist; Head, Andrology
Laboratory, Institute of Reproductive Medicine of the University, Münster,
Germany (with Prof. E. Nieschlag)
Society Memberships
American Andrology Society (1990)
British Andrology Society (1979)
Deutsche
Gesellschaft für Andrologie (2000)
European Academy of Andrology (1992)
European Biomedical Research Association
(1995)
Institute of Biology (1983)
Research Defence Society (1980)
Society for the Study of Fertility
(1979)
Great Britain-China Centre
Society for Anglo-Chinese Understanding
Grants awarded
1980-1983 Wellcome Trust Fellowship in
Veterinary Medicine (3 years: own salary and consumables)
1984-1985 M.R.C. Research Fellowship (18
months: own salary and consumables)
1986-1988 D.F.G. Research Grant Co
142/1-1 (2 years: technician and consumables)
1989-1991 D.F.G. Research Grant Ni
130/11-1 (C) (3 years: scientist, technician and consumables)
1992-1994 D.F.G. Research Grant Ni
130/11-2 (C) (3 years: scientist, technician and consumables)
1994-1996 G.I.F. Research Grant
I-225-002.02/92 (3 years, half technician and consumable)
1995-1997 D.F.G. Research grant Co
142/6-1 (2 years, one student, technician, consumables)
1996-1997 D.F.G. Research grant Ni
130/15 (3 years, one scientist, technician, consumables)
1996-1998 IKF Research Grant D1 (2
years, one student, technician, consumables)
1997-1999 Rockefeller Foundation/Ernst
Schering Research Foundation (3 years, one technician, 1 Post Doc, consumables)
1997-2000 D.F.G. Research grant Ni
130/15 (3 years, one scientist, technician, consumables)
1997-2000 Volkswagen Stiftung I (3
years, consumables, travel)
1998-2001 IKF/IZKF Research Grant D1-2
(4 years, one scientist, 1 technician, consumables)
2001-2002 D.F.G: Research grant FOR
197/3-1 (2 years, one scientist, 1 technician, consumables)
2000-2002 Rockefeller Foundation/Ernst
Schering Research Foundation (2 years, one technician, 1 Post Doc, consumables)
2001-2002 Volkswagen Stiftung II (2
years, consumables, travel)
2003-2004 CONRAD/Ernst Schering Research
Foundation (2 years, one technician, 1 Post Doc, consumables)
Supervision of Student Theses
Diploma thesis
G. Oberländer: Experimentelle Untersuchungen zur Befruchtungsphysiologie
der männlichen Ratte (Rattus norvegicus)
unter Verwendung der Fertilitätshemmers Ornidazol. 1991. Experimental
studies on the physiology of fertilisation in the male rat (Rattus norvegicus)
under the influence of the antifertility compound ornidazole. 1991.
S. Weydert. Vergleichende
Untersuchungen zur Spermienreifung bei Cavia aperea und Galea musteloides, zwei
Meerschweinchenarten mit unterschiedlingem Paarungssystem. 1998. Comparative
studies of sperm maturation in Cava aperea and Galea musteloides, two guinea
pig species with different mating systems. 1998.
M.D. theses
T. Gudermann. Sekretion von myo-Inositol und
L-carntine in das Lumen der Cauda epididymidis der Ratte. 1989. Secretion of carnitine and inositol into the lumen of the cauda
epididymidis of rat. 1989.
A. Burgert. Direktionale Molekültransport durch
kultivierte Nebenhodenepithel der Ratte. 1993. Directional
molecular transport across cultured epididymal epithelial cells. 1993.
D. Wenker. Zellkultur des Nebenhodenepithels der
maturen Ratte auf einer permeablen Filtermembran. 1993. Culture
of adult rat epididymal epithelial cells on a permeable membrane. 1993.
R. Meyer. Anzüchtung immaturer
Rattennebenhodenepithelzellen in einer statischen Monolayerkultur auf
permeablen Trägern. 1994. Secretory activity of rat epididymal
epithelial cells in static monolayer culture on permeable supports. 1994.
K. Mennicke. Vergleich der Morphologie und Motilität
von humanen Spermien bei verschiedene Gefriermethoden mit computerassistierte
Spermienanalyse. 1994. Comparison of the morphology and motility of
human sperm after different freezing protocols with computer-aided sperm
analysis. 1994.
U.
Oberdieck. Einfluss von Karenzzeit und Ejakulatfrequenz auf die Zusammensetzung
des Ejakulats. 1996. Influence of abstinence time and ejaculation frequency on the
composition of the ejaculate. 1996.
M. Wulfhorst. Modifikation der Aufthaumethode. 1997. Modification of thawing methods. 1997.
M. Suter. Immunohistochemische Darstellung des “Contraception associated
protein 1”, CAP1. 2001. Immunocytochemical
localisation of ‘Contraception associated protein 1’, CAP1. 2002.
C. Avram. Relationship of
the proto-oncogene c-ros to the development of the initial segment in
the murine epididymis. In preparation
S. Fetic. Cytoplasmic droplets of human spermatozoa observed
in wet and fixed preparations and their involvement in volume regulation of
human spermatozoa. Work in progress
Dr.rer.nat. theses
M. Hölpert. Über die Messung von -laktalbumin,
Galaktosytransferase und pyrophosphatase im Nebenhoden der Ratte. 1993. Measurement of -lactalbumin, galactosyltransferase and pyrophosphatase in the rat
epididymis. 1993.
S. Raczek. Proteinsynthese und Sekretion von
Nebenhodenepithelzellen der Ratte und des Menschen in der in vitro Kulture. 1993. Protein synthesis and secretion on epididymal epithelial cells from the
rat and man in in vitro culture. 1993.
A. Wagenfeld. Zur Physiologie der Fertilität.
Experimentelle Untersuchungen am Nebenhoden fertiler und infertiler Ratten. 1997. On the
physiology of fertilisation. Experimental studies of the epididymis of fertile
and infertile rats. 1997.
W.
Bone. Untersuchungen zur Glycolyse von Säugerspermien und der Bedeutung für die
Fertilität. 1999. Studies on glycolysis by vertebrate sperm and its importance for
fertility. 1999.
J. Barfield. Volume
regulating sperm channels as contraceptive targets.
Publications in
Journals (175, 65 as first author)
1999
Gago C, Tablado
L, Yeung C-H, Pérez-Sánchez F, Cooper TG, Soler C (1999) Morphological
characterization of ejaculated monkey (Macaca
fascicularis) spermatozoa. Am J Primatol, 47, 105-115
Cooper TG, Atkinson AD, Nieschlag E (1999)
Experience with external quality control in spermatology. Human Reproduction,
14, 765-769
Yeung
C-H, Weinbauer GF, Cooper TG (1999) Effect of acute androgen withdrawal
by GnRH antagonist on epididymal sperm motility and morphology in the
cynomolgus monkey. J Androl, 20, 72-79
Jones
AR, Cooper TG. (1999) A re-appraisal of the post-testicular
antifertility action and toxicity of chlorinated antifertility compounds. Int J
Androl, 22, 130-138
Rolf
C, Cooper TG, Yeung C-H, Nieschlag E (1999) Antioxidant treatment of
patients with asthenozoospemia or moderate oligoasthenozoospermia with high
dose vitamin C and vitamin E: a randomized, placebo-controlled, double-blind
study. Human Reproduction, 14, 1028-1033
Cooper
TG, Yeung CH (1999) Recent chemical approaches to post-testicular,
epididymal contraception. Human Reproduction Update, 5, 141-152
Cooper
TG, Yeung CH (1999) Approaches to post-testicular contraception. Asian
Journal of Andrology, 1, 29-36
Yeung CH, Weinbauer GF, Cooper TG (1999) Responses of monkey
epididymal sperm of different maturational status to secondary messengers
mediating protein tyrosine kinase phosphorylation, acrosome reaction and motility.
Mol Reprod Develop, 54, 194-202
Yeung
C-H, Sonnenberg-Riethmacher, E, Cooper, TG (1999) Infertile spermatozoa
of c-ros tyrosine kinase receptor
knockout mice show flagellar angulation and maturational defects in cell volume
regulatory mechanisms. Biol Reprod, 61, 1062-1069
Cooper
TG (1999) Immunology of the epididymis. Andrologia 31, 322
Seiler
P, Cooper TG, Yeung C-H, Nieschlag E (1999) Regional variation in
macrophage expression by murine epididymal basal cells and their regulation by
testicular factors. J Androl, 20, 738-746
2000
Bone
W, Jones NG, Kamp G, Yeung, CH, Cooper TG. (2000) Effect of ornidazole
on fertility of male rats: inhibition of a glycolysis-related motility pattern
and zona binding required for fertilisation in
vitro. J Reprod Fert, 118, 127-135
Cooper
TG, S Weydert, C-H Yeung, C Künzl, N Sachser (2000). Maturation of epididymal spermatozoa in
the non-domesticated guinea pigs Cavia
aperea and Galea musteloides, J
Androl, 21, 154-163.
Seiler
P, Cooper TG, Nieschlag E (2000) Sperm number and condition affect the
number of basal cells and their expression of macrophage antigen in the murine
epididymis. Int J Androl, 23, 65-76.
Soler C, Pérez-Sánchez F, Schulze H, Bergmann M,
Oberpenning F, Yeung C-H, Cooper TG (2000) Objective evaluation of the
morphology of human epididymal sperm heads. Int J Androl, 23, 77-84
Gago
C, Soler C, Pérez-Sánchez F, Yeung C-H, Cooper TG (2000) Effects of
Cetrorelix on sperm morphology during migration through the epididymis in the
cynomolgus macaque (Macaca fascicularis).
Am J Primatol, 51, 103-117
Cooper
TG, Jones AR (2000) The
metabolism of the putative antifertility agents 3-36chloro-1-hydroxypropanone
and its dimethyl ketal in the male rat. Int J Androl, 23, 243-247
von
Eckardstein S, Cooper TG, K. Rutsch, D. Meschede, J. Hörst, E. Nieschlag
(2000) Seminal plasma characteristics as indicators of cystic fibrosis
transmembrane conductance regulator (CFTR) gene mutations in men with
obstructuve azoospermia. Fertil Steril, 73, 1226-1231
Yeung C-H, Wagenfeld A, E. Nieschlag & Cooper TG
(2000) The cause of infertility of c-ros
tyrosine kinase knockout male mice. Biol Reprod, 63, 612-618
Bone
W and Cooper TG (2000) In vitro
inhibition of rat cauda epididymal sperm glycolytic enzymes by ornidazole, a-chlorohydrin and 1-chloro-3-hydroxypropanone. Int J Androl, 23, 243-247
Cooper
TG (2000) Die Reifung humaner Spermien im Nebenhoden. Reproduktionsmedizin. 16, 299-309
Wagenfeld
A, Yeung, C-H, Shivaji S, Sundareswaran VR, Aragi H, Cooper TG (2000)
Expression and cellular localization of contraception asociated protein 1.
J Androl, 21, 954-963.
Yeung
CH, S Schröter, C Kirchhoff, Cooper TG (2000) Maturational changes of
the CD52-like epididymal glycoprotein on cynomolgus monkey sperm and their
apparent reversal in capacitation conditions. Mol Reprod Dev 57, 280-289
Bharadwaj A, Sengupta A,
Ghosh I, Cooper TG, Weinbauer GF, Brinkworth M, Nieschlag E, Ghebrehiwet
B, Peerschke EIB, Datta K (2000) Stage specific expression of the proprotein
form of HABP1 during spermatogenesis in the rat. Mol Reprod Develop, 62, 223-23
2001
Bone
W, Jones AR, Morin C, Nieschlag E, Cooper TG (2001) Susceptibility to
inhibition of glycolytic enzyme activity and motility of spermatozoa from the
rat, mouse and man by proven and putative chlorinated antifertility compounds in vitro. J Androl 22, 464-470
Cooper
TG (2001) Epididymal maturation of human spermatozoa. Chinese Nat J Androl
7, 1-9.
Derr
P, Yeung C-H, Cooper TG, Kirchhoff C (2001) Synthesis and glycosylation
of CD52, the major ‘maturation-associated’ antigen of rat spermatozoa, in the
cauda epididymidis. Reproduction 121, 435-446.
Yeung
CH, Pérez-Sánchez F, Schröter S, Kirchhoff C, Cooper TG (2001) Changes
of the major sperm maturation-associated epididymal protein HE5 (CD52) on human
ejaculated spermatozoa during incubation. Mol Human Reprod 7, 617-624.
2002
Cooper
TG, Yeung C-H, Jones R, Orgebin-Crist, M-C, Robaire B (2002) Rebuttal of a
role for the epididymis in sperm quality control by phagocytosis of defective
sperm. J Cell Sci, 115, 5-7.
Bone
W, Jones AR, Nieschlag E, Cooper TG (2002) The effect of (R,S)-ornidazole
on the fertility of male mice and the excretion and metabolism of 36Cl-(R,S)
a-chlorohydrin in male mice and rats. Int J Androl, 25, 94-99.
Jones
AR, Morin C, Bone W, Nieschlag E, Cooper TG (2002) Lack of antifertility
properties of novel halogenated glycolytic inhibitors and the urinary excretion
and metabolism of 1,6-dichloro-1,6-dideoxy-fructofuronose in the male rat. Int
J Androl, 25, 168-174.
Christova
Y, James PS, Cooper TG, Jones R (2002) Lipid diffusion in the plasma
membrane of mouse spermatozoa. Changes during epididymal maturation, effects of
pH, osmotic pressure and knockout
of the c-ros gene, J Androl, 23,
384-392.
Yeung
CH, Anapolski M, Cooper TG (2002) Measurement of volume changes in mouse
spermatozoa using an electronic sizing analyser and a flow cytometer:
validation and application to an infertile mouse model. J Androl, 23, 522-528.
Yeung
CH, Anapolski M, Sipilä P, Wagenfeld A, Poutanen M, Huhtaniemi I, Nieschlag E, Cooper
TG (2002) Sperm volume regulation: maturational changes in fertile and
infertile transgenic mice and association with kinematics and tail angulation.
Biol Reprod, 67, 269-275.
Cooper
TG, Vreeburg J, Björndahl L, Nieschlag E (2002). Semen analysis and external quality control schemes for semen analysis
need global standardisation. Int J Androl, 25:306-311.
Wagenfeld
A, Yeung C-H, Lehnert W, Nieschlag E, Cooper TG (2002) Lack of glutamate
transporter EAAC1 in the epididymis of infertile c-ros receptor tyrosine-kinase deficient mice. J Androl, 23:772-82.
Sipilä
P, Cooper TG, Yeung C-H, Mustonen M, Penttinen J, Drevet J, Huhtaniemi
I, Poutanen M (2002) Epididymal dysfunction initiated by the expression of
Simian Virus 40 T-antigen leads to angulated sperm flagella and infertility in
transgenic mice. Mol Endocrinol, 16, 2603-2617
Hohoff C, Solmsdorff K,
Löttker P, Kemme K, Epplen JT, Cooper TG, Sachser N (2002). Monogamy in
a new species of wild guinea pigs. Naturwissenschaften, 89, 462-465.
2003
Xu Y-X, Wagenfeld A, Yeung C-H, Lehnert W, Nieschlag E, Cooper TG
(2003a) Expression and location of the taurine transporter in the epididymis of
infertile c-ros receptor tyrosine
kinase-deficient and fertile heterozygous mice. Mol Reprod Develop, 64, 144-151
Cooper
TG, Yeung C-H (2003a) Acquisition of volume regulatory response of sperm
upon maturation in the epididymis and the role of the cytoplasmic droplet.
Microscopy Research & Technique, 61, 28-38.
Soler
C, de Monserrat JJ, Gutiérrez R, Nuñez J, Nuñez F, Pérez-Sánchez F, Cooper
TG (2003) Use of the Sperm-Class Analyser® for objective
assessment of human sperm morphology. Optimisation of sperm preparation. Int J
Androl, 26, 262-270
Andersen
OM, Yeung C-H, Vorum H, Wellner M, Andreassen TK, Erdmann B, Mueller E-C, Herz
J, Otto A, Cooper TG, Willnow TE (2003) Essential role of the
Apolipoprotein E receptor-2 in sperm development. J Biol Chem, 278, 23989-23995
Xu
YX, Yeung C-H, Setiawan I, Avram C, Biber J, Wagenfeld A, Lang F, Cooper TG
(2003b) Sodium-inorganic phosphate cotransporter NaPi-IIb in the
epididymis and its potential role in male fertility studied in a transgenic
mouse model. Biol Reprod, 69, 1135-1141
Baart
EB, van der Heijden GW, van der Hoeven FA, Bakker R, Cooper TG, de Boer
P (2004) Reduced oocyte activation and first cleavage rate after ICSI with
spermatozoa from a sterile mouse chromosome mutant. Hum Reoprod 19, 1140-1147.
Avram
CE, Cooper TG (2004) Development of the caput epididymidis studied by
expressed proteins (a glutamate transporter, a lipocalin and b-galactosidase)
in the c-ros knockout and wild-type
mice with prepubertally ligated efferent ducts. Cell Tiss Res, 317, 23-34.
Avram
CE, Yeung, C-H, Nieschlag E, Cooper TG (2004) Regulation of the initial
segment of the murine epididymis by dihydrotestosterone and testicular exocrine
secretions studied by expression of
specific proteins and gene expression. Cell Tiss Res 317, 13-22.
Yeung
CH, Barfield JP, Anapolski M, Cooper TG (2004) Volume regulation of
mature and immature spermatozoa in a primate model and possible ion channels
involved. Human Reproduction, Epub ahead of print.
Publications in Books (27, 16 as first
author)
1999
Cooper
TG (1999) Epididymis. Encyclopedia of Reproduction. Neill, J,D; Knobil, E,
Editors. Academic Press, San Diego. Vol. 2, pp. 1-17
Yeung
C-H, Sonnenberg-Riethmacher E, Cooper TG (1998) Receptor tyrosine kinase
c-ros knockout mice as a model for
the study of epididymal regulation of sperm function. The Epididymis: cellular
and molecular aspects. Eds RC Jones, MK Holland, C Doberska. J Reprod Fert
Suppl 53, 137-147
Cooper
TG, Yeung C-H (1999) Sperm maturation in the primate epididymis. Weinbauer GF, Korte
R (eds.) Reproduction in nonhuman primates, Waxmann Verlag, Münster, 61-78
Nieschag
E, Weinbauer GF, Cooper TG, Wittkowski W (1999) Reproduktion. In
Physiologie, Edn 3, pp. 521-539, Deetjen, P, Speckmann, E-J (eds) Urban &
Schwarzenberg, München.
2000
Weinbauer
GFW, Cooper TG (2000) Assessment of male fertility impairment in the macaque model, Corte R; Weinbauer GF,
(eds.) Towards new horizons in primate toxicology, Waxman Verlag, Münster,
pp.13-42
Cooper
TG, Yeung, C-H (2000) Physiologie der Spermienreifung und Fertilisation.
In: Andrologie. Grundlagen und Klinik der reproduktiven Gesundheit des Mannes.
E. Nieschlag, H.M. Behre, editors. Springer, Berlin, pp. 69-90.in press
Cooper
TG Yeung, C-H (2000) Physiology of sperm maturation and fertilization. In
Andrology. Male reproductive health and dysfunction. Nieschlag E, Behre HM, Eds.
Springer, Berlin, pp. 63-82.
2002
Cooper
TG (2002) The epididymis as a target for male contraception. In The
Epididymis. From molecules to clinical practice. A comprehensive survey of the
efferent ducts, the epididymis and the vas deferens. Robaire B, Hinton BT, eds.
Kluwer Academic/Plenum Publishers, New York, pp.483-502.
Yeung
C-H, Cooper TG (2002) Acquisition and development of sperm motility upon
maturation in the epididymis. In The Epididymis. From molecules to clinical
practice. A comprehensive survey of the efferent ducts, the epididymis and the
vas deferens. Robaire B, Hinton BT, eds. Kluwer Academic/Plenum Publishers, New
York, pp.417-434.
Cooper
TG (2002) Epididymal approaches to male contraception. In Andrology in the
21st century. Proceedings of the VIIth International Congress of
Andrology, Montréal. B.
Robaire, H Chemes, C.R. Morales Eds. Medomond
Medical Publications, New Jersey, pp. 499-509.
Yeung
C-H, Cooper TG (2002) Cell Volume Regulation by Mammalian Spermatozoa.
In: Proceedings of the 9th International Symposium on Spermatology (Cape
Town, October 2002). Van der Horst, G; Franken, D; Bornman, R; De Jager, T;
Dyer, S (editors), Medimond Inc, Bologna, pp. 167-172.
2003
Cooper
TG, Yeung C-H (2003b) Approaches to post-testicular contraception: insights
from the infertile c-ros knockout
mouse. In: The third international conference on the Epididymis. Hinton BT,
Turner TT, eds., Van Doren Company, Charlottesville. III. pp. 208-221
Yeung, C-G, Cooper TG
(2003) Developmental changes in signalling transduction factors in maturing
sperm during epididymal transit. In: Signal transduction and Gamete Function,
Special Issue of Cellular and Molecular Biology. 49, 341-349, Guest Editor S
Shivaji.