Work Address: (VA
Medical Center) (LSU
Health Sciences Center)
Research
Service (151) Department of Biochemistry
Overton
Brooks VA Med Center
and Molecular Biology
510
E. Stoner Ave. 1501
Kings Highway, PO Box 33932
Shreveport,
LA 71101-4295 Shreveport,
LA 71130-3932
Telephone: 318-211-8411
ext. 6685
FAX: 318-429-5734
e-mail: sgrime@lsuhsc.edu, Sidney.Grimes@va.med.gov, and srgrimes@prysm.net
Education: University
of North Carolina, Chapel Hill,
North
Carolina (Chemistry) B.S., 1969
University
of North Carolina, Chapel Hill,
North
Carolina (Biochemistry) Ph.D., 1973
University
of Texas, Houston, Texas
(Postdoctoral
Research, Biochemistry) 1973-1975
Vanderbilt
University, Nashville, Tennessee
(Postdoctoral
Research, Biochemistry) 1975-1976
Academic and Professional Experience:
1997-present Research Professor of Biochemistry
and
Molecular
Biology, Louisiana State University
Health
Sciences Center and Research Chemist, VA Medical
Center,
Shreveport, Louisiana.
1/1999-3/2000 Acting
ACOS for Research and Development, VA Medical Center
1988-1997 Research
Associate Professor of Biochemistry and
Molecular
Biology, Louisiana State University
Health
Sciences Center and Research Chemist, VA Medical
Center,
Shreveport, Louisiana.
1984 Visiting
scientist for two months in the laboratory
of
Drs. Gary and Janet Stein, Department of
Biochemistry
and Molecular Biology, University of
Florida,
Gainesville, Florida.
1976-1988 Assistant
Professor of Biochemistry and Molecular
Biology,
Louisiana State University Health Sciences Center, and
Research
Chemist, VA Medical Center, Shreveport, Louisiana
1975-1976 Research
Associate, Vanderbilt University,
Nashville,
Tennessee
1974-1975 Research
Associate, University of Texas
System
Cancer
Center, M.D. Anderson Hospital and Tumor Institute
1973-1974 Project Investigator,
Department of Biochemistry,
The
University of Texas System Cancer Center, M.D.
Anderson
Hospital and Tumor Institute
1969-1973 Research
Assistant in Biochemistry, University of
North
Carolina, Chapel Hill, North Carolina
Awards: A
National Research Service Award sponsored by the U.S.
Public
Health Service administered by Vanderbilt
University
Reproductive Biology, Biochemistry and
Endocrinology.
Cited in American Men and Women of Science
Cited
in Marquis Who's Who in Science and Engineering
Fields of Interest: Regulation
of gene expression. The role of nuclear proteins in
regulating
transcription during cellular differentiation and
development.
Scientific Societies: American
Society for Cell Biology - Active
Sigma
Xi - Active
American
Society for Microbiology - Active
American
Assoc. for the Advancement of Science - Active
Society
for the Study of Reproduction - Active
Teaching Experience:
Biochemistry-
Supervised three dental students in Biochemistry research project,
Fall,
1972 and Spring, 1973, Univ. North
Carolina.
Developmental
Biology Course - Graduate student lectures, Dept. Biochemistry,
Univ.
TX, 1973.
Biochemistry
of Nuclear Proteins Course - Graduate student lectures, 1975.
Biochemistry-
Lectures and laboratory in the medical Biochemistry course
in
the Dept. Biochemistry and Molecular Biology, 1976 - 1984.
Graduate
Biochemistry course lectures (1993 - present), (13 hr 2002)
LSUHSC, Shreveport.
Nucleic
Acids course. Two-thirds of
lectures in this one
semester
graduate course, Dept.
Biochemistry
and
Molecular Biology, LSUHSC - Shreveport, spring, 1980.
Cell
Biology- Lectured in this interdepartmental graduate course
in
the Dept. Biochemistry and Molecular Biology,
LSUHSC,
Shreveport, 1984.
Biochemical
Analytical Methods - Presently
lecture in this graduate
course.
Alternate
years (1989 - 1993), Every year (4 hr/yr; 1994 - present).
Dept.
Biochem. and Molec. Biol., LSUHSC., Shreveport.
Editorial Boards: Molecular
Biology Reports (Kluwer Academic
publishers) 1996
- Present
Reproductive
Biology and Endocrinology (BioMed Central Assoc.
Electronic
Journals) 2003 - Present.
Biology
of Reproduction July, 2003 - present.
Committees:
(VA Medical Center)
Research
and Development Committee - 1977
- 1980
Research
Evaluation Subcommittee - 1977
- 1980
Radioisotope
Committee - 1977
- 1980
Subcommittee
on Animal Studies - 1977
- 1987
Subcommittee
on Animal Studies - 1993
- 1998
Subcommittee
on Research Safety - 1980
- Present
Chairman,
Subcommittee on Research Safety - 1983
- Present
Chairman,
Subcommittee on Animal Studies - 1980
- 1987
Chairman,
Subcommittee on Animal Studies - 1993
- 1998
Chairman,
Research Biosafety Subcommittee - 1985
- 1994
Research
Biosafety committee - 1994
- Present
Subcommittee
on Human Studies - 1990
- 1991
Subcommittee
on Human Studies - 1993
- 1995
VA
Medical Center Safety Committee - 1996
- 1967
VA
Radiation Safety Committee -
1996
- Present
VA
Hazardous Materials Subcommittee - 1996
- Present
Research
and Development Committee - 1999
- 2000
Research
and Development Committee - 2002
- Present
Medical
Library Advisory Committee - 1999
- 2000
VISN
16 Data Privacy Board - 2002
- Present
(LSU Health Sciences Center)
Elected
Faculty Council - 1988
- 1990
Research
Advisory Committee
for
ACS and Cancer Center Grants: - 1992
Research
Advisory Committee (Peer Review grants)- 1993
-1998
Faculty
Search Committee - 1994
Graduate
Student Admissions Committee - 1994
- 1997
Graduate
Student Admissions Committee - 1998
- 2001
Biosafety
Committee (and
RAS Member) - 1995
– 6/1999
Biosafety
Committee (RAS Member only) - 6/1999
Departmental
Library Committee - 2001
- present
Animal
Care and Use Committee - 2003
- present
(Graduate Student Committees)
Biochemistry
and Molecular Biology
Marcie
Fowler, Ph.D. 2002-
present
Kenneth R. Noss, Ph.D. 1998-2002 Major Advisor (Grad.
8/02)
Donald
C.Wilkerson, Ph.D. 1998-2002
Major Advisor (Grad. 8/02)
Kevin
P. Pavlick, Ph.D 1996-2000 (Graduated 12/00)
Claudia
Orellana, M.S. 1999-2000
Heather
Panek, Ph.D. 1994-1999 (Graduated 6/99)
Micheal
Wheeler, Ph.D. 1998-1999
Donald C.Wilkerson, M.S. 1996-1998 Major Advisor
(Grad. 6/98)
Peter
Mottram ,
M.S. 1996-1998
Major Advisor (Grad.12/98)
Jane vanWert, Ph.D. 1994-1997 Major Advisor (Grad. 6/97)
Guofeng
Xie, Ph.D. 1991-1995 (Graduated 6/95)
Cletus
D'Souza, Ph.D. 1991-1995
”
Inez
Pinto, Ph.D. 1988-1992 ”
Christopher
Adams, Ph.D. 1988-1992 ”
Jong
Na, Ph.D. 1988-1992 ”
Dan
Ware, M.S. 1988-1991 ”
Microbiology
and Immunology
Aaron
Martin, Ph.D. 2002-
present
Shu-Jan
Lee, Ph.D. 1987
Susan
A. Key, M.S. 1985
Thomas
F. Bittick, M.S. 1985
Lawton
A. Seal, Ph.D. 1985
Medical
Students
Jeffrey
Anderson 1988-1990
Philip
Andrew Rascoe 1997
(Am.Can.Soc.Research
Fellowship
Recipient)
Graduate
Student Rotations
Kenneth
Noss 1997-98
(Jan-March)
Micheal
Wheeler 1997
(May-July)
Peter
Mottram 1997 (March-June)
Donald
C. Wilkerson 1995-96
(December-March)
Peggy
Merchant 1994
(March-June)
Heather
Panek 1994
(March-June)
Jane
vanWert 1993
(June-Aug.)
Postdoctoral
Fellows Trained in my laboratory - Steven
A. Wolfe, Ph.D.
- Jane
vanWert, Ph.D.
Currently
mentor for Rakesh Singal, M.D. 1998-present
Dr.
Singal has received four funded grants during my service as his
mentor:
a VA Merit Review Grant (active), a DOD grant (active 2002),
an
NIH grant (active 2002), and a VA Career Development Award (2002).
I
have agree to mentor Subhashini Yaturu, M.D. 2004 - present
Publications:
(Full
Length Papers)
1. S.R.
Grimes, 1973. "Studies on Rat
Testis Histones," Title of Thesis for Ph.D. in Biochemistry, University of
North Carolina, Chapel Hill, North Carolina.
2. S.R.
Grimes, C-B. Chae, and J.L. Irvin, 1975.
"Acetylation of Histones of Rat Testis." Archives of
Biochemistry and Biophysics. 168: 425-435.
3. R.E.
Branson, S.R. Grimes, G. Yonuschot, and J.L. Irvin, 1975."The Histones of
Rat Testis." Archives of Biochemistry and Biophysics. 168: 403-412.
4. S.R.
Grimes, C-B. Chae, and J.L. Irvin, 1975.
"Effects of Age and Hypophysectomy Upon Relative Proportions of
Various Histones in Rat Testis."
Biochem. Biophys. Res. Comm. 63: 911-917.
5. S.R.
Grimes, R.D. Platz, M.L. Meistrich, and L.S. Hnilica, 1975. "Partial
Characterization of New Basic Nuclear Protein from Rat Testis Elongated
Spermatids." Biochem.
Biophys. Res. Comm. 67: 182-189.
6. R.D.
Platz, S.R. Grimes, M.L. Meistrich, and L.S. Hnilica, 1975. "Changes in
Nuclear Proteins of Rat Testis Cells Separated by Velocity Sedimentation."
J. Biol. Chem. 250: 5791-5800.
7. S.R.
Grimes, M.L. Meistrich, R.D. Platz, and L.S. Hnilica, 1977. "Nuclear
Protein Transitions in Rat Testis Spermatids." Expt'l Cell Res. 110:
31-39.
8. M.L.
Meistrich, S.R. Grimes, R.D. Platz, W.A. Brock, and L.S. Hnilica, 1977. "Molecular Events in
Spermatogenesis." The Cancer Bulletin. 29: 12-13.
9. M.L.
Meistrich, W.A. Brock, S.R. Grimes,
Jr., R.D. Platz, and L.S. Hnilica, 1978. "Nuclear Protein Transitions During Spermatogenesis."
Federation Proceedings 37: 2522-2525.
10. M.L.
Meistrich, J.L. Longtin, W.A. Brock,
S.R. Grimes, and M.L. Mace, 1981.
"Purification of Rat Spermatogenic Cells and Preliminary
Biochemical Analysis of These Cells." Biol. of Reproduction 25: 1065-
1077.
11. S.R.
Grimes, Jr. and N. Henderson. 1983. "Acetylation of Histones During
Spermatogenesis in the Rat" Arch. Biochem. Biophys. 220:108-116.
12. S.R.
Grimes, Jr. and N. Henderson. 1984. "Acetylation of Rat Testis Histones
H2B and TH2B." Developmental Biology 101:516- 521.
13. S.R.
Grimes. 1984. "Release of Nuclear Ribonucleoprotein During Brief Digestion
of Rat Testis Chromatin with Deoxyribonuclease II (3.1.4.6)" Compar.
Biochem. Biophys. 78B:633-641.
14. S.R.
Grimes, Jr. and N. Henderson. 1984. "Hyperacetylation of Histone H4 in Rat
Testis Spermatids." Exp. Cell Res. 152:91- 97.
15. S.R.
Grimes, Jr. and P. Smart, 1985. "Changes in the Structural Organization of
Chromatin During Spermatogenesis in the Rat." Biochim. Biophys. Acta. 824:128-139.
16. S.R.
Grimes, Jr., 1986. "Nuclear Proteins in Spermatogenesis." Compar.
Biochem. Biophys. 83B:495-500.
Publications: (Full
Length Papers)
17. P.
Weisz-Carrington and S.R. Grimes, Jr., and M.L. Lamm, 1987. "Gut
Associated Lymphoid Tissue as A Source of an IgA Immune Response in Respiratory
Tissues After Oral Immunization and Intrabronchial Challenge." Cellular
Immunology. 106:132-138.
18. S.R.
Grimes, P. Weisz-Carrington,
H. Daum, J. Smith, L. Green, K. Wright, G.S. Stein, and J.L. Stein, 1987. "A Rat Histone H4
Gene Closely Associated with
the Testis-Specific H1t Gene." Exptl. Cell Res. 173:534-545.
19. S.A.
Wolfe, J.V. Anderson, S.R. Grimes, G.S. Stein, and J.L. Stein, 1989.
"Comparison of the Structural Organization and Expression of Germinal and
Somatic Rat Histone H4 Genes."
Biochem. Biophys. Acta 1007:140-150.
20. S.R.
Grimes, S.A. Wolfe, J.V. Anderson, G.S. Stein, and J.L. Stein, 1990. "Structural and Functional
Analysis of the Rat Testis-Specific Histone H1t Gene." J. Cellular
Biochem. 44:1-17.
21. T.A.
Owen, J.Holthuis, E.Markose, A.J. van Wijnen, S.A. Wolfe, S.R. Grimes, J.B.
Lian, and G. Stein, 1990 "Modifications of Protein-DNA Interactions in the
Proximal Promoter of a Cell Growth Regulated Histone Gene During the Onset of
Osteoblast Differentiation." PNAS USA 87:5129 - 5133. (Communicated by
David Prescott).
22. S.A.
Wolfe and S.R. Grimes, 1991. "Protein-DNA Interactions within the Rat
Histone H4t Promoter." J. Biol. Chem. 266:6637-6643.
23. S.R.
Grimes, S.A. Wolfe, and D.A. Koppel, 1992. "Tissue-Specific Binding of
Testis Nuclear Proteins to a Sequence Element within the Promoter of the Testis-Specific Histone H1t
Gene." Arch. Biochem.
Biophys. 296:402-409.
24. S.R.
Grimes, S.A. Wolfe, and D.A. Koppel, 1992. "Temporal Correlation between
the Appearance of Testis-Specific DNA-Binding Proteins and the Onset of
Transcription of the Testis-Specific Histone H1t Gene." Exptl. Cell Res. 201:216-224.
25. M.W.
Owens and S.R. Grimes, 1993. "Pleural Mesothelial Cell Response to
Inflammation: Tumor Necrosis Factor-Induced Proliferation and Collagen
Synthesis." Amer. J. Physiology: Lung Cellular and Molecular Physiology
265:L382-L388.
26. S.A.
Wolfe and S.R. Grimes, 1993. "Histone H1t: A Tissue-Specific Model used to
Study Transcriptional Control and Nuclear Function During Cellular
Differentiation." J. Cell. Biochem. 53:156-160.
27. S.R.
Grimes, S.A. Wolfe, D.A. Koppel, and L. Fogelfeld. "Regulation of
Expression of Mammalian Testis Histone Genes." Trends in Comparative Biochemistry and Physiology, 1:
(1993) pp.365-375.
28. D.A.
Koppel, S.A. Wolfe, L. Fogelfeld, P. Merchant, L. Prouty, and S.R. Grimes,
1994. "Primate Testicular Histone H1t Genes are Highly Conserved and the
Human H1t Gene is Located on Chromosome 6" J. Cellular Biochemistry.
54:212-222.
29. S.A.
Milligan, M.W. Owens, R.J. Henderson, and S.R. Grimes, 1995.
"Characterization of Proteoglycans Produced by Rat Pleural Mesothelial
Cells in vitro." Exp. Lung. Res. 21:559-575.
30. J.
VanWert, S.A. Wolfe, and S.R. Grimes, 1995. "Testis-Specific Expression of
the Rat Histone H1t Gene in Transgenic Mice". Biochemistry 34:8733-8743.
Publications: (Full
Length Papers )
31. S.A.
Wolfe, vanWert, and S.R. Grimes, 1995. "Expression of the Testis-Specific Histone
H1t Gene: Evidence for Involvement of Multiple Cis-acting Promoter
Elements". Biochemistry
34:12461-12469.
32. J.
VanWert, S.A. Wolfe, and S.R. Grimes, 1996. "Binding of Nuclear Proteins
to a Conserved Histone H1t Promoter Element Suggests an Important Role in
Testis-Specific Transcription". J. Cellular Biochemistry 60:348-362.
33. S.R.
Grimes, S.A. Wolfe, and J. vanWert, 1997. "Regulation of Transcription of
the Testis-Specific Histone H1t Gene by Multiple Promoter Elements".
Molecular Biology Reports 24:175-184.
34. J.M.
vanWert, H.R. Panek, S.A. Wolfe, and S.R. Grimes, 1998. "The TE Promoter
Element of the Histone H1t Gene is Essential for Transcription in Primary
Spermatocytes". Biology of Reproduction. 59:704-710.
35. S.A.
Wolfe and S.R. Grimes, 1999. "Binding of Nuclear Proteins to an Upstream
Element Involved in Transcriptional Regulation of the Testis-Specific Histone
H1t Gene". J. Cellular
Biochemistry 75:555-565.
36. S.A.
Wolfe, P.J. Mottram, J.M. vanWert and S.R. Grimes, 1999. "Localization of
Upstream Elements Involved in Transcriptional Regulation of the Rat
testis-Specific Histone H1t Gene in Somatic Cells". Biology of
Reproduction 61:1005-1011.
37. D.
Good, P.O. Schwarzenberger, J.A. Eastham, R.E. Rhoads, J.D. Hunt, M. Collins, M. Batzer,
C. Theodossiou, J.K. Kolls, and
S.R. Grimes, 1999. "Cloning and Characterization of the Prostate-Specific
Membrane Antigen Promoter". J. Cellular Biochemistry 74:395-405.
38. R.
Singal, J.M. vanWert, M. Bashambu, S.A. Wolfe, D.C. Wilkerson and S.R. Grimes,
2000. "The Testis-Specific Histone H1t Gene is Hypermethylated in
Nongerminal Cells". Biology of Reproduction 63:1237-1244.
39. R.
Singal and S.R. Grimes, 2001. "Microsoft® WordTM Macro for
Analysis of Cytosine Methylation by the Bisulfite Deamination Reaction".
BioTechniques 30(1): 116-120.
40. S.R.
Grimes, S.A. Wolfe and R. Singal, 2001. "Macro for Analysis of CpG and
CpNG Methylation in Plants". BioTechniques 30(6): 1248.
41. K.R.
Noss, S.A. Wolfe, and S.R. Grimes, 2002. "Upregulation of Prostate
Specific Membrane Antigen (PSMA)/Folate Hydrolase (FOLH1) Transcription by an
Enhancer". Gene 285: 247-256.
42. K.
Noss, R. Singal, and S.R. Grimes, 2002. "Methylation Status of the
Prostate Specific Membrane Antigen (PSMA) CpG Island in Prostate Cancer Cell
Lines". Anticancer Research 22: 1505-1512.
43. D.C.
Wilkerson, S.A. Wolfe, and S.R. Grimes, 2002. "The H1t/GC-box and H1t/TE1
element are essential for promoter activity of the Testis-Specific Histone H1t
Gene". Biology of Reproduction 67: 1157-1164.
44. D.C.
Wilkerson, S.A. Wolfe, and S.R. Grimes, 2002. "Sp1 and Sp3 activate the
testis specific histone H1t promoter through the H1t/GC-box". J. Cellular Biochem. 86:716-725.
Publications: (Full
Length Papers )
45. S.R.
Grimes, D.C. Wilkerson, K.R. Noss and S.A. Wolfe, 2003. "Transcriptional
Control of the Testis-Specific Histone H1t Gene". GENE 304:13-21.
46. D.C.
Wilkerson, S.A. Wolfe, and S.R. Grimes, 2003. "The TE2 and TE1
sub-elements of the testis-specific histone H1t promoter are functionally
different". J. Cellular
Biochem. 88:1177-1187.
47. S.A.
Wolfe and S.R. Grimes, 2003. "Transcriptional Repression of the
Testis-Specific Histone H1t gene Mediated by an Element Upstream of the
H1t/AC-Box". Gene
308:129-138.
48. S.A.
Wolfe and S.R. Grimes, 2003. "Specific Binding of Nuclear Proteins to a
Bifunctional Promoter Element Upstream of the H1/AC Box of the Testis-Specific
Histone H1t Gene". Biol. Reprod. 68:2267-2273.
49. S.A.
Wolfe, D.C. Wilkerson, S. Prado and S.R. Grimes, 2004. "Regulatory Factor
X2 (RFX2) Binds to the H1t/TE1 Promoter Element and Activates Transcription of
the Testis-Specific Histone H1t Gene ". J. Cellular Biochem. 91:375-383.
50. S.
Yaturu, S. Prado, and S. Grimes, 2004. "Changes in Adipocyte Hormones
Leptin, Resistin and Adiponectin in Thyroid Dysfunction". J. Cellular
Biochem. (In Press).
51. S.R.
Grimes, 2004. "Testis-Specific Gene Expression". GENE (In Press).
52. S.R.
Grimes, S. Prado, S.A. Wolfe, 2004. "Transcriptional Activation of the
Testis-specific Histone H1t Gene by RFX2 May Require Both Proximal Promoter
X-Box Elements ". J. Cellular Biochem. (In Press).
53. S.R.
Grimes, S. Prado, S.A. Wolfe, 2004. "Expression of RFX Family Members
During Spermatogenesis". Mol. Biol. Reports. (In Preparation).
54. S.R.
Grimes, 2004. "Enhancer elements involved in regulating PSMA Promoter
Activity in a prostate cancer cell line ". Mol. Biol. Reports (In
Preparation).
55. S.R.
Grimes, 2004. "Use of RNAi to Regulate PSMA Promoter Activity in a
prostate cancer cell line ". Asian Journal of Andrology (In Preparation).
(67 abstracts - see next page for list)
Publications: (Chapters
in Books)
1. R.D.
Platz, S.R. Grimes, G. Hord, M.L. Meistrich, and L.S. Hnilica. "Changes in
Nuclear Proteins During Embryonic Development and Cellular
Differentiation." In Chromosomal Proteins and Their Role in the Regulation
of Gene Expression (G.S. Stein and L.J. Kleinsmith, ed.) Academic Press, New York, 1975, pp.67-92.
2. R.D.
Platz, S.R. Grimes, M.L. Meistrich, and L.S. Hnilica. "Low Molecular
Weight Basic Proteins in Spermatids." Methods in Chromosomal Protein
Research, Vol. 16 in the series Methods in Cell Biology, (G.S. Stein and L.J. Kleinsmith, ed.)
Academic Press, New York, 1977,pp.297- 316.
3. L.S.
Hnilica, S.R. Grimes, and J-F. Chiu.
"Electrophoretic Fractionation of Histones Utilizing Starch Gels
and Sodium Dodecyl Sulfate Urea Gels." Methods in Chromosomal Proteins
Research, Vol 17 in the series Methods in Cell Biology. (G.S. Stein and L.J.
Kleinsmith, ed.) Academic Press, 1978,pp.211- 222.
4. M.L.
Meistrich, W.A. Brock, S.R. Grimes, Jr., R.D. Platz, and L.S. Hnilica. "Control of Synthesis of Specific
Gene Products During Spermatogenesis." Cell Differentiation and Neoplasia (G.F. Saunders, ed.) Raven Press, New York, 1978,pp.404-412.
5. S.R.
Grimes, D.C. Wilkerson, K.R. Noss and S.A. Wolfe, 2003. "Regulation
of transcription of the testis histone H1t gene". Invited
chapter for the
Transworld Research Network series 'Recent
Research Developments in Cellular Biochemistry'.
1:117-129.
Publications:
(Abstracts)
1. S.R.
Grimes, Jr. Irvin, and C-B. Chae, 1973. "Acetylation of Rat Testis Nuclear
Proteins." Protein Phosphorylation in Control Mechanisms. Miami Winter Symposia (Ed. F. Huijing
and E.Y.C. Lee) Vol. 5, p. 321,Academic Press, New York.
2. S.R.
Grimes, G. Jahnke (Spon: R. Penniall), 1973. "Acetylation of Rat Testis
Nuclear Proteins." Fed. Proc. 32: 587. Abs. #2090.
3. R.E.
Branson, S.R. Grimes, G. Yonuschot, and J.L. Irvin, 1973. "Rat Testis Histones." Fed.
Proc. 32: 588. Abs. #2096.
4. R.D.
Platz, S.R. Grimes, M.L. Meistrich, G.R. Hord, and L.S. Hnilica, 1974.
"Changes in Nuclear Proteins During Spermatogenesis in the Rat.” Fed. Proc. 33: 1411.
5. S.R.
Grimes, R.D. Platz, M.L. Meistrich, and L.S. Hnilica, 1974. "Nuclear Proteins
During Spermatogenesis in the Rat Testis." J. Cell Biol. 63(2): 124a.
6. S.R.
Grimes, Jr., R.D. Platz, M.L. Meistrich, and L.S. Hnilica, 1975. "Nuclear
Protein Transitions During Spermatogenesis in the Rat." J. Cell Biol. 67:146a.
7. R.D.
Platz, S.R. Grimes, Jr., M.L.
Meistrich, and L.S. Hnilica, 1976. "Synthesis and Phosphorylation of Nuclear
Proteins in Rat Testis Spermatids." J. Cell Biol. Abstract, 70, 106.
8. S.R.
Grimes, K. Sakuma, K. Hardy, J-F. Chiu, F. Chytil, and L.S. Hnilica, 1976. "The Specific Structure and
Function of DNA-Protein Complexes
of Chicken Reticulocyte Chromatin." Cell Biol. Abstract, 70, 367.
9. S.R.
Grimes, M.N. Blackburn, and R.J. Wilson, 1978. "Studies on Transcriptionally Active and Inactive
Chromatin from Chicken Erythroid Cells." J. Cell Biol.
Abstract, 79, 123a.
10. S.R.
Grimes and R.J. Wilson, 1979. "The Nature of Chromatin Fractions from
Avian Erythroid Cells." Fed. Proc. 38:492.
11. S.R.
Grimes and D.E. Kay, 1979.
"Assay of Rat Testis Polysomal and Postribosomal Supernatant RNA
for Messenger RNA Activity." J. Cell Biol. 83, 221a.
12. S.R.
Grimes, M.L. Meistrich, and D. Kay, 1980.
"RNA Synthesis in Highly Purified Rat Testis Pachytene
Spermatocytes and Early
Spermatids." J. Cell
Biol. 87, 150a. Publications: (Abstracts)
13. M.L.
Meistrich, L.R. Bucci, W.A. Brock, P.K. Trostle, R.D. Platz, S.R. Grimes, and
B.D. Burleigh, 1980.
"Characterization of TP4, A Major Nuclear Protein of Rat Elongated
Spermatids. Fed. Proc. 39:1884.
14. S.R.
Grimes, Jr. (Spon: J.Manno), 1981.
"Age Dependence of Levels of Unusual Acid-Soluble Proteins in Rat
Testis Chromatin Fractions." Fed. Proc. 40: 1569a.
15. S.R.
Grimes, Jr. and Jo Ann Cameron, 1981. "Release of Ribonucleoprotein From Chromatin By
Digestion with DNase II." J.
Cell Biol. 91: 59a.
16. S.R.
Grimes, Jr. and N. Henderson, 1982. "Acetylation of Histones In Rat Testis
Spermatids." J. Cell Biol.
95: 169a.
Publications: (Abstracts)
17. S.R.
Grimes, Jr. and N. Henderson, 1983. "Acetylation and Deacetylation of
Histones During Spermatogenesis in the Rat." Southeastern Cancer Research
Association. Med. and Pediatric Oncol. 11:65.
18. S.R.
Grimes, Jr., 1983. "Acetylation of Histones H2B and TH2B in Rat Testis."
J. Cell Biol. 97:14.
19. S.R.
Grimes, Jr. and P. Weisz-Carrington, 1984."Evidence of Specific Adherence
of IGA-Bearing Lymphocytes to Intestinal Secretory Epithelium." Fed. Proc.
43:383.
20. S.R.
Grimes, Jr. and P. Weisz-Carrington, 1984."Increase in Anti- Ferritin
Specific Lymphocytes in Tracheal and Bronchial Tissues of Orally Immunized Mice
after Intrabronchial Challenge with Ferritin." Fed. Proc. 43:727.
21. S.R.
Grimes, Jr., 1984. "A Possible Role for Hyperacetylation of Histone H4 in
the Removal of Histones from DNA in Rat Testis Spermatids." Fed. Proc.
43:2063.
22. S.R.
Grimes, Jr. and P. Smart, 1984. "Histone H4 Hyperacetylation Lowers the
Thermal Stability of Chromatin During Spermatogenesis in the Rat. Southeastern
Cancer Research Association. 12th Ann. Meeting, Oct. 1984.
23. S.R.
Grimes, Jr., P. Smart, K. Wright, L. Green, P. Laipis, G. Stein, and J.
Stein,1985. "Isolation and Partial Characterization of Rat Core and H1
Histone Genes. J. Cell Biol. 101:312.
24. S.R.
Grimes, Jr., P. Smart, J. Smith, H. Daum III, K. Wright, L. Green, G. Stein,
and J. Stein, 1985, "Partial Characterization of a Rat Histone H4
Gene." Southeastern Cancer Research Association. 13th Ann. Meeting, Oct. 1985.
25. S.R.
Grimes, Jr., J.M. Smith, H. Daum, L. Green, K. Wright, G.S. Stein and J.L.
Stein, 1986. "Nucleotide Sequence and Genomic Organization of A Rat
Histone H4 Gene." J. Cell Biol. 103:312a.
26. P.
Weisz-Carrington and S.R. Grimes, 1987. "Specific Attachment of Stable
Murine Monoclonal MN, Hybrids to the Endothelium of the Gut Lamina Propria
Capillaries." Fed. Proc.
46:1220a.
27. S.R.
Grimes, Jr., J. Anderson, G.S.
Stein, and J.L. Stein, 1987. "Expression of the Closely Associated Histone
Genes H1t and H4 During Spermatogenesis in the Rat. J. Cell Biol. 105:150a.
28. S.R.
Grimes, S.A. Wolfe, J.V. Anderson,
G.S. Stein, and J.L. Stein,
1988. "Comparison of the Structural Organization and Expression of
Germinal and Somatic Rat Histone H4 Genes." J. Cell Biol.
107:754a.
29. S.A.
Wolfe, S.R. Grimes, J.V. Anderson, G.S. Stein, and J.L. Stein, 1988.
"Expression of the Rat Testis Histone H1t Gene During Spermatogenesis and
in Transfected NIH/3T3 Cells." J. Cell Biol. 107:754a.
30. J.V.
Anderson, S.R. Grimes, and S.A. Wolfe, 1988. "Expression of Rat
Testis-Specific Histone H1t Protein A Fusion Protein in E.coli and Utilization
to Prepare Specific Antibodies for Detection of Histone H1t by Western Blot
Analysis." J. Cell Biol. 107:748a.
31. S.A.
Wolfe, J.V. Anderson, and S.R. Grimes, 1989. "Transient Expression of Rat
Testis Histone H1t and Rat Somatic H4 CAT Fusion Genes in Transfected
Cells." J. Cell Biol. 109:287a.
Publications:
(Abstracts)
32. S.A.
Wolfe and S.R. Grimes, 1990. "Transcriptional Regulation of the Rat-Testis
Specific Histone H1t Gene." J. Cell Biol. 111:219a.
33. M.W.
Owens and S.R. Grimes, 1991. "Pleural Mesothelial Cell Procollagen mRNA is
Increased by Tumor Necrosis Factor." Am. Thoracic Soc.
34. S.R.
Grimes, S.A. Wolfe, and D.A. Koppel, 1991. "Testis-Specific Protein-DNA Interactions
within the Histone H1t Promoter." J. Cell Biol. 115:152a.
35. S.A.
Wolfe, S.R. Grimes, and D.A. Koppel, 1991. "Correlation between the
Premeiotic Appearance of Testis-Specific DNA-Binding Proteins and the
Transcription of the Testis-Specific Histone H1t Gene During
Spermatogenesis." J. Cell Biol. 115:152a.
36. W.McD.
Anderson, S.R. Grimes, and J.H. Zavecz, 1992. "Inositol 1,4,5,
Trisphosphate Receptor mRNA Expression in the Diaphragm." The FASEB
Journal 6:A1240.
37. D.A.
Koppel, S.A. Wolfe, L. Fogelfeld, and S.R. Grimes, 1992. "Amplification
and Sequence Comparison of the Human and Rhesus Monkey Testicular Histone H1t
Genes using PCR." Mol. Biol. of the Cell 3:101a.
38. S.A.
Wolfe, D.A. Koppel, L. Fogelfeld, and S.R. Grimes, 1992. "PCR Amplification,
Cloning and Sequencing the Mouse Testis-Specific Histone H1t Gene." Mol.
Biol. of the Cell 3:81a.
39. S.A.
Wolfe, D.A. Koppel, L. Fogelfeld, and S.R. Grimes, 1992. "Identification
of Protein Binding to a Conserved DNA Sequence Element within the Proximal
Promoter of Mammalian H1t Genes." Mol. Biol. of the Cell 3:324a.
40. S.R.
Grimes, J. vanWert, D.A. Koppel, J. Wright, S.A. Wolfe, and L. Prouty, 1993. "PCR Analysis of Expression
of the Testis-specific Histone H1t Gene and Mapping the Human Histone H1t Gene
to Chromosome 6." Mol. Biol.
of the Cell 4:29a.
41. S.A.
Wolfe and S.R. Grimes, 1993. "Modular Analysis of a Conserved Region
within Mammalian Histone H1t Promoters." Mol. Biol. of the Cell 4:195a.
42. J.M.
vanWert, S.A. Wolfe, and S.R. Grimes, 1994. "Tissue-Specific Expression of
the Rat Histone H1t in Transgenic Mice." Joint Annual Meeting of the Amer.
Soc. Microbiol. South Central Branch and Mid-South Biochemists, in Shreveport,
LA.
43. J.M.
vanWert, H.R. Panek, P.S. Merchant, S.A. Wolfe, and S.R. Grimes, 1994. "A
Conserved Mammalian Histone H1t Promoter Element Suggests an Important Role in
Testis-specific Transcription." Mol. Biol. of the Cell 5:221a.
44. S.A.
Wolfe, J.M. vanWert, and S.R. Grimes, 1994. "Analysis of a Highly
Conserved Region within the Histone H1t Promoter: Evidence for Multiple
Cis-acting Testis Elements." Mol. Biol. of the Cell 5:221a.
45. J.M.
vanWert, H.R. Panek, S.A. Wolfe, and S.R. Grimes, 1995. "Conserved
Transcriptional Regulatory Sequence Elements Contribute to Tissue-specific
Expression of the Rat H1t Gene in Transgenic Mice." Mol. Biol. of the Cell
6:77a.
46. S.A.
Wolfe, J.M. vanWert, and S.R. Grimes, 1995. "Localization of Elements
Involved in Regulation of Transcription of the Testis-specific Rat Histone H1t
Gene." Mol. Biol. of the Cell 6:77a.
Publications:
(Abstracts)
47. J.
vanWert, H.R. Panek, S.A. Wolfe, and S.R. Grimes, 1996. The Promoter Element
Designated TE Is Essential For Testis-Specific Transcription Of The Rat Histone
H1t Gene (6th International Congress on Cell Biology) Mol. Biol. of the Cell
7:619a.
48.
D.C. Wilkerson, S.A. Wolfe, J.M. vanWert, and S.R.
Grimes, 1997. Characterization of Enriched Fractions Containing the
Testis-specific Histone H1t/TE Element Binding Protein. Mol. Biol. of the Cell
8:220a.
49. S.A.
Wolfe, J. vanWert, and S.R. Grimes, 1997. Characterization of the Histone H1t
Promoter Using Luciferase Reporter Constructs. Mol. Biol. of the Cell 8:326a.
50. J.
vanWert, D.C. Wilkerson, P. J.Mottram, S.A. Wolfe, and S.R. Grimes, 1997.
Analysis of Cis-acting Elements Within the Rat Histone H1t Proximal Promoter.
Mol. Biol. of the Cell 8:326a.
51. S.R.
Grimes, R. Rhoads, J. Eastham, W. Gan, P. Rascoe, an W.B. Murrill, 1998.
Regulation of Transcription of the Prostate-Specific Membrane Antigen (PSMA)
Gene. Keystone Symposia "Molecular and Cellular Biology of Gene
Therapy", Keystone, Colorado Jan., 1998.
52. R.
Rhoads, S.R. Grimes, and J.
Eastham, 1998. Regulation of
Transcription of the Prostate-Specific Membrane Antigen (PSMA) Gene. Keystone
Symposia "Prostate Cancer and Breast Cancer" Keystone, Colorado Feb., 1998.
53. D.C.
Wilkerson, S.A. Wolfe, J.M. vanWert, and S.R. Grimes, 1998. Characterization of
Enriched Fractions Containing the Testis-specific Histone H1t/TE Element Binding
Protein. Graduate Research Day, LSUMC.
54. S.A.
Wolfe, J. vanWert, and S.R. Grimes, 1998. Characterization of the Histone H1t
Promoter Using Luciferase Reporter Constructs. Graduate Research Day, LSUMC.
55. J.
vanWert, D.C. Wilkerson, P. J.Mottram, S.A. Wolfe, and S.R. Grimes, 1998.
Analysis of Cis-acting Elements Within the Rat Histone H1t Proximal Promoter.
Graduate Research Day, LSUMC.
56. P.J.
Mottram, D.C. Wilkerson, J.M. vanWert, S.A. Wolfe, S.R. Grimes, 1998. Evidence
For Tissue-Specific Binding of Nuclear Proteins to the Histone H1t Promoter.
(Abst.#1025) Mol. Biol. of the Cell 9(supplement):177a.
57. S.A.
Wolfe, P.J. Mottram, J.M. vanWert, and S.R. Grimes. 1998. Localization of an
Upstream Element Involved In the Repression of Histone H1t In Somatic Cells.
(Abst.#1027) Mol. Biol. of the Cell 9(supplement):178a.
58. J.M.
vanWert, S.A. Wolfe, P.J. Mottram, D.C. Wilkerson, S.R. Grimes. 1998. The TE
Enhancer Is Essential For Regulation of Transcription of the H1t Gene.
(Abst.#1026) Mol. Biol. of the Cell 9(supplement):177a.
59. K.Noss,
R. Rhoads, S.R. Grimes, and J.
Eastham, 1999. Regulation of
Transcription of the Prostate-Specific Membrane Antigen (PSMA) Gene. Fourth
Annual Ray A. Barlow Scientific Symposium: Gene Therapy in Louisiana. Jan. 29,
1999.
Publications:
(Abstracts)
60. D.C.
Wilkerson, S.A. Wolfe and S.R. Grimes. 1999. Identification and
Characterization of Proteins that Bind to the H1t/TE Element of the
Testis-Specific Histone H1t gene. (Abst.#1189) Mol. Biol. of the Cell 10
(supplement):205a.
61. S.A.
Wolfe and S.R. Grimes. 1999. Identification of a Potential Regulatory Element
within the Testis-Specific Histone H1t Promoter. (Abst. #1190) Mol. Biol. Of
the Cell 10 (supplement):205a.
62. K.
Noss, S.R. Grimes. 2000. Studies on the Effect of Androgens on the Promoter
Activity of the Prostate Specific Membrane Antigen (PSMA) Gene in Prostate
Cancer Cells. (Abst. #2260) Mol. Biol. of the Cell 11 (supplement):435a.
63. D.C.
Wilkerson, J. vanWert, R. Singal and S. Grimes. 2000. Hypermethylation of the
Testis-specific Histone H1t Gene May Contribute to Transcriptional Repression
in Nongerminal Cells. (Abst. #1613) Mol. Biol. of the Cell 11
(supplement):310a.
64. S.
Wolfe and S. Grimes, 2000. Binding of nuclear proteins to an upstream element
within the Histone H1t Proximal Promoter Correlates with Transcription. (Abst.
#1614) Mol. Biol. of the Cell 11 (supplement):310a.
65. D.C.
Wilkerson, S.A. Wolfe, and S.R. Grimes, 2001. Multiple Regulatory Mechanisms
Modulate Transcriptional Activation and Silencing of the Testis Specific
Histone H1t Gene. (Abst. #546) Mol. Biol. of the Cell 12 (Supplement) 100a.
66. K.R.
Noss, S.A. Wolfe, and S.R. Grimes, 2001. Analysis of the Prostate Specific
Membrane Antigen (PSMA) Promoter and Enhancer's Effect on Transcription in
Prostate Cell Lines. (Abst. #1238) Mol. Biol. of the Cell 12 (Supplement) 227a.
67. S.
Yaturu, S. Prado, and S.R. Grimes, 2003. Effect of Thyroid dysfunction on
adipocyte hormones. (Presented to Endocrine Society Oct. 8, 2003).
(55 full length papers and book chapters)
Grant Support: VA
Merit Reviews 8
funded from 1977 – 2003 ($2,052,000)
NIH 3
funded from 1978 – 2001 ($1,066,500)
Other
($
9,000)
Total
($3,127,530)
Veterans Administration Merit Review Project - Principal Investigator -
"Regulation of Gene Expression by Nonhistone Chromosomal Proteins".
1977 - 1980, $80,000/yr.
NIH grant - Principal Investigator - "Basic
Spermatidal Protein mRNA in Rat Testis". 1978 - 1981, $125,000/yr.
VA Merit Review Project - Principal Investigator - "The Role of Nuclear
Proteins In Erythroid Cells".
1980 - 1984. $100,000/yr.
VA Merit Review Project - Principal Investigator
- "Role of Histone
Acetylation in Rat Testis Spermatids." 1984 - 1987, $97,000/yr.
LSUMC BRSG grant - Principal Investigator - "Histone Gene Switching
During Mammalian
Spermatogenesis." 1985 - 1986. $4000.
Type IV VA Merit Review - Principal Investigator -
"Histone Gene Switching During Mammalian Spermatogenesis." 1986 -
1987. $25,000.
VA Merit Review Project - Principal Investigator -
"Regulation of Histone Gene Expression During Mammalian
Spermatogenesis." 1987 - 1990. $166,000.
VA Merit Review Project - Principal Investigator -
"Regulation of Histone Gene Expression During Mammalian
Spermatogenesis." 1990 - 1994. $342,700.
NIH grant - Principal Investigator - "Regulation
of Transcription of the Histone H4t Gene". 1993 - 1996. $311,730.
VA Merit Review Project - Principal Investigator -
"Mechanisms Regulating Expression of the Testis Histone H1t Gene."
1996 - 1999. $314,700.
INTRAMURAL RESEARCH SUPPORT AWARD LSUMC-S-BRF -
Principal Investigator - "Evaluation of Tissue-Specific Transcription by
the Promoter of the Prostate Specific Membrane Antigen (PSMA) Gene."
January 1998 - June 1999. $5000.
NIH grant - Principal Investigator -
"Transcriptional Regulation During Spermatogenesis." Aug, 1997 -
July, 2001. $379,800.
VA Merit Review Project - Principal Investigator -
"Mechanisms Regulating Expression of the Testis Histone H1t Gene."
10/2000 – 9/2003. $338,600.
VA Merit Review Project - Principal Investigator -
"Mechanisms Regulating Expression of the Testis Histone H1t Gene."
4/2005 – 3/2008. $325,000 (To be Submitted Sept 2004)
NIH grant 1 RO1 HD044567-01- Principal Investigator -
"Transcriptional Regulation During Spermatogenesis." 6/2005 - 5/2008.
$325,000 (To be Submitted Oct 2004).
NIH grant - Principal Investigator - "
Tissue-Specific Promoters for Prostate Cancer Therapy." (to be submitted).