Lin, Ching-Shwun

Current Title & Associate Professor,

Department: Department of Urology, University of California

Mailing Address: Box 1695

University of California

San Francisco, CA 94143

Telecommunication: (415) 353-7205 (office)

(415) 353-9586 (fax)

clin@urol.ucsf.edu (e-mail)

Education

Dates Institution and Location Degree Major

1973-1978 National Taiwan University, Taiwan DVM Veterinary Medicine

1979-1983 University of Iowa, Iowa City, IA Ph.D. Microbiology

Certifications

1978 Certificate for Practice of Veterinary Medicine

Professional Positions Held

Dates Institution Position

1978 Department of Microbiology, National Taiwan University Research Assistant

1978-1979 Department of Microbiology, National Taiwan University Teaching Assistant

1979-1983 Department of Microbiology, University of Iowa Teaching Assistant

1983-1988 Department of Molecular Oncology, Linus Pauling Institute Research Scientist

1988-1991 California Institute for Medical Research Senior Scientist

1991-1993 Palo Alto Medical Foundation/Research Institute Senior Scientist

1993-1997 Department of Stomatology, University of California, SF Molecular Biologist

1997-2000 Department of Urology, University of California, SF Assistant Professor

1997-present Molecular Urology Laboratory, UCSF Research Director

2000-present Department of Urology, University of California, SF Associate Professor

Honors and Awards

1976 Representative of students, National Taiwan University.

1976 President Award, National Taiwan University

1976 Pfizer Award for Outstanding Academic Achievements

1977 Taiwan poultry farm scholarship

1979-1983 NIH Cellular and Molecular Biology Training Grant

1983-1985 Postdoctoral fellowship

2001 Presidential Award for Outstanding Research, 4^th Annual Congress of Chinese Andrology Society.

Professional Membership

1988-present American Association for the Advancement of Science

1994-present The New York Academy of Sciences

1995-Present The Proteome Society

2001-present International Society for Sexual and Impotence Research

2002-present Society for Basic Urological Research

2003-present American Urological Association

Intramural Services

2002 Member, PhD student thesis committee, Department of Stomatology, UCSF

2000 Member, PhD student thesis committee, Department of Stomatology, UCSF

2001 Member, PhD student thesis committee, Department of Stomatology, UCSF

Extramural Services

1990 Grant Reviewer, Swiss National Science Council

1988-present Reviewer for several biomedical journals

1999-present Member, Animal Care and Use Committee, Notre Dame de Namur University

2001 Grant Reviewer, The Netherlands Research Council

2003 Committee member, 2nd International Consultation on Erectile and Sexual Dysfunction, Paris, France.

Invited Speakership

1999 Identification of Three PDE5 Isoforms in Human Corpus Cavernosum. GlaxoWellcome Corporation. Research Triangle Park, North Carolina, USA.

1999 Cloning of PDE5 cDNAs and Gene Promoter. Novartis Corporation. Horsham, England.

2000 Application of Molecular Biology to Impotence Research. 9^th World Meeting on Impotence Research. Perth, Australia.

2001 Who Needs Viagra? Chinese Bioscience Association. Los Altos, California, USA.

2001 Regulation of PDE5A Gene by Sildenafil and Nitroprusside. PDE5 Roundtable. Napa, California, USA.

2001 How to Apply Molecular Biology to Impotence Research. Fourth Annual Chinese Andrology Society National Conference. Hanzhou, China.

2002 Human PDE5A Gene Expression and Regulation. Gordon Research Conference, South Hadley, Massachusetts, USA.

2002 Human PDE5A Gene Expression and Regulation. PDE Think Tank Meeting, Dublin, Ireland.

2003 Human PDE5A Gene Expression and Regulation. PDE Think Tank Meeting, Halifax, Canada.

2003 2nd International Consultation on Erectile and Sexual Dysfunction, Paris, France.

Patents and Inventions

1988 US and European Patents: Plastin Isoforms and Their Uses

Pending US patent: Phosphodiesterase-5A Isoforms

Pending US and European Patents: Methods and Compositions for Preventing and Treating Erectile and Sexual Dysfunction

Pending Combination Growth Factor Therapy for Neurogenic and Vasculogenic Impotence

Teaching Experience

1978-1979 Two semesters of Medical Microbiology Lab

1978-1979 One semester of Immunology Lecture

1979-1981 Four semesters of Medical Microbiology Lecture and Lab

1981-1983 One semester of Immunology Lecture and Lab

1993-1995 Lectures and Lab, 2 hours/week, Anatomy 118, General Histology

1995-present Lab instruction 10 hours/week, Molecular Biology/Urology

Graduate Students Supervised

1995-1996 Li-Yin (Ricking) Chiu

1995-present Ming-Guang Yeh

2001-present Eric Schwandt

Postdoctoral fellows Supervised

1986-1988 Madhu Varma

1988-1990 Karabi Ghosh

1991-1993 Taesun Park

1992-1993 Zong Ping Chen

1998-1999 Che-Chung Yeh

1999-2001 Xiaowei Liu

2001-present Guiting Lin

Postdoctoral Fellows Trained

1993-1996 Ken Zhang

1993-1995 Kentaro Matsumoto

1994-1996 JaneYao

1995-1997 Ming-Guang Yeh

1996-1997 Mutsuhiko Murai

1997-1999 Emre Bakircioglu

1998-1999 Ming-Chan Lee

1999-2001 Hao-Chung Ho

2001-2002 Kuo-Chiang Chen

2001-2002 Amr Jad

2001-present Zhong Wang

Research Grants

(All dollar amounts are direct costs)

Years	PI	Source	Amount	Program Title
1988-1993 1989-1992 1992-1993 1993-1994 1994-1995 1994-1995 1994-1995 1995-1998 1996-2001 1998-1999 1998-2001 1998-2002 1999-2004 2000-2001 2001-2002 2002-2003 2002-2007 2002-2003 2003-2007 (pending) 2003-2007 (pending) 2003-2004 (pending) 2004-2005 (pending)	J. Leavitt C.-S. Lin C.-S. Lin C.-S. Lin C.-S. Lin C.-S. Lin C.-S. Lin C.-S. Lin R. Kramer C.-S. Lin T. F. Lue T. F. Lue M. Berger C.-S. Lin C.-S. Lin C.-S. Lin J. Brown C.-S. Lin C.-S. Lin T. F. Lue C.-S. Lin C.-S. Lin	NIH NIH Chapman Research Fund UC Academic Senate NIH SPORE UC Academic Senate UC CRCC California BCRP NIH Calif. Urol. Foundation NIH NIH NIH Calif. Urol. Foundation Calif. Urol. Foundation Calif. Urol. Foundation NIH Bayer NIH NIH Mt. Zion Health Fund NIH	$635,278 $220,326 $18,643 $17,550 $18,183 $14,530 $30,000 $304,802 $485,677 $15,000 $800,000 $1,000,305 $2,163,573 $15,000 $15,000 $15,000 $4,220,015 $100,000 $700,000 $1,250,000 $100,000 $100,000	Role of Microfilament Proteins in Neoplasia and Metastasis Role of Metaschematin in Neoplasia Role of Integrins in Neoplasia Regulation of L-plastin Gene Promoter Role of L-plastin in Breast Cancer L-plastin Function and Regulation in Cancer Role of a6 Integrin in Breast Cancer Role of L-plastin in Breast Cancer Laminin and its Receptors in Oral Cancer Molecular Mechanisms of Peyronie’s Disease Treatment and Prevention of Impotence Molecular Mechanisms of Female Stress Urinary Incontinence Program for Treatment of Malignant Brain Tumor Characterization of VEGF and VEGF Receptors Human PDE5A Gene Expression and Regulation An in vitro Model of Nerve Regrowth Program for Female Urinary Incontinence Effects of Vardenafil on PDE5 Promoter and Isoforms Role of PDE5A in Erectile Dysfunction Vascular Endothelial Growth Factor and Impotence Molecular Analysis of Female Urinary Incontinence Models Role of Mast Cells in Interstitial Cystitis

Publications (Peer-reviewed articles only; abstracts and case reports not included. Year in red indicates corresponding authorship)

1984 Lin, C.-S. Nucleotide sequence of the essential region of bacteriophage P4. Nucleic Acids Research. 12: 8667-8684.

1984 Leavitt, J., Gunning, P., Porreca, P., Ng, S.-Y., Lin, C.-S., and Kedes, L. Molecular cloning and characterization of mutant and wild-type human b-actin genes. Mol. Cell Biol. 4: 1961-1969.

1984 Calendar, R., Kahn, M., Six, E., Goldstein, R., Lindqvist, B., Deho, G., Inman, R., Jiang, R.-Z., Lin, C.-S., Lee, S.-J., Lagos, R., Christie, G., Dale, E., and Pierson, L. Genetic map of bacteriophage P4. in Genetic Maps 1984, Cold Spring Harbor Laboratory.

1985 Lin, C.-S., Ng, S.-Y., Gunning, P., Kedes, L., and Leavitt, J. Identification and order of sequential mutations in b-actin genes isolated from increasingly tumorigenic human fibroblast strains, Proc. Natl. Acad. Sci. USA. 82: 6995-6999.

1985 Kedes, L., Ng, S.-Y., Lin, C.-S., Gunning, P., Eddy, R., Shows, T., and Leavitt, J. The human beta-actin multigene family. Transactions of the Association of American Physicians 98: 42-46.

1988 Lin, C.-S., and Leavitt, J. Cloning and characterization of a cDNA encoding transformation-sensitive tropomyosin isoform 3 from tumorigenic human fibroblasts, Mol. Cell Biol. 8: 160-168.

1988 Lin, C.-S., Aebersold, R. H., Kent, S. B., Varma, M., and Leavitt, J. Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts. Mol. Cell Biol. 8: 4659-4668.

1990 Lin, C.-S., Aebersold, R. H., and Leavitt, J. Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction:

identification of a potential calcium-binding domain. Mol. Cell Biol. 10:1818-1821.

1990 Gunning, P., Gordon, M., Wade, R., Gahmann, R., Lin, C.-S., and Hardeman, E. Differential control of tropomyosin mRNA levels during myogenesis suggests the existence of an isoform competition-autoregulatory compensation control mechanism. Developmental Biol. 138: 443-453.

1990 Halling, C., Calendar, R., Christie, G. E., Dale, E. C., Finkel, S., Flensburg, J., Kahn, M. L., Lane, K. B., Lin, C.-S., Lindqvist, B. H., Pierson, L. S., Six, E. W., Sunshine, M. G., and Ziermann, R. DNA sequence of satellite bacteriophage P4. Nucleic Acids Research 18: 1649.

1990 de Arruda, M., Watson, S., Lin, C.-S., Leavitt, J., and Matsudaira, P. Fimbrin is a homolog of the cytoplasmic phosphoprotein plastin and has domains homologous with calmodulin and actin gelation proteins. J. Cell Biol. 111: 1069-1079

1993 Lin. C.-S., Park, T., Chen, Z. P., Ghosh, K., and Leavitt, J. Human plastin genes: comparative gene structure, chromosome location, and differential expression in normal and neoplastic cells. J. Biol. Chem. 268: 2781-2792

1993 Lin, C.-S., Chen, Z. P., Park, T., and Leavitt, J. Characterization of the human L-

plastin gene promoter in normal and neoplastic cells. J. Biol. Chem. 268: 2793-2801.

1993 Novy, R. E., Liu, L.-F., Lin, C.-S., Helfman, D. M., and Lin, J. J. C. Expression of smooth muscle and nonmuscle tropomyosins in Escherichia coli and characterization of bacterially produced tropomyosins. Biochimica et Biophysics Acta 1162: 255-265.

1993 Lin, C.-S., Zhang, K., and Kramer, R. H. a6 integrin is up-regulated in step-increments accompanying neoplastic transformation and tumorigenic conversion of human fibroblasts. Cancer Res. 53: 2950-2953.

1993 Ziober, B. L., Vu, M. P., Waleh, N., Crawford, J., Lin, C.-S., and Kramer, R. H. Alternative extracellular and cytoplasmic domains of the integrin a7 subunit are differentially expressed during development. J. Biol. Chem. 268: 26773-26783.

1993 Novy, R. E., Liu, L.-F., Lin, C.-S., and Lin, J. J. C. Human fibroblast tropomyosin isoforms: characterization of cDNA clones and analysis of tropomyosin isoform expression in human tissues and in normal and transformed cells. Cell Motil. Cytoskeleton. 25: 267-281.

1994 Lin, C.-S., Shen, W., Chen, Z. P., Tu, Y.-H., and Matsudaira, P. Identification of I-plastin, a human fimbrin isoform expressed in intestine and kidney. Mol. Cell Biol. 14: 2457-2467.

1995 Adams, A, E. M., Shen, W., Lin, C.-S., Leavitt, J., and Matsudaira, P. Isoform-specific complementation of the yeast sac6 null mutation by human fimbrin. Mol. Cell Biol. 15: 69-75.

1996 Ziober, B. L., Lin, C.-S., and Kramer, R. H. Laminin-binding integrins in tumor progression and metastasis. Seminars in Cancer Biology 7: 119-128.

1997 Lin, C.-S., Chang, C.-H., and Huynh, T. The murine L-plastin gene promoter:

Identification and comparison with the human L-plastin gene promoter DNA and Cell Biol. 16: 9-16.

1997 Beviglia, L., Matsumoto, K., Lin, C.-S., Ziober, B. L., and Kramer, R. H. Expression of the c-Met/HGF receptor in human breast carcinoma: correlation with tumor progression. International J. Cancer 74: 301-309.

1997 Lin, C.-S., Chen, Y. Q., Huynh, T., and Kramer, R. H. Identification of the human a6 integrin gene promoter. DNA and Cell Biol. 16: 929-937.

1997 Lin, C.-S., A method to reverse the order of multiple cloning sites. BioTechniques 23:

220-221.

1998 Lin, C.-S., Lau, A. and Lue, T. F. Analysis and mapping of plastin phosphorylation. DNA and Cell Biol. 17: 1041-1046.

1998 Lin, C.-S., Lau, A., Bakircioglu, E., Tu, R., Wu, F., Week, S., Nunes, L., and Lue, T. F. Analysis of Neuronal Nitric Oxide Synthase Isoform Expression and Identification of Human nNOS-m. Biochem. Biophyis. Res. Comm. 253: 388-394.

1999 Lin, C.-S., Lau, A., Huynh, T., and Lue, T. F. Differential regulation of human T-plastin gene in leukocytes arid non-leukocytes: Identification of the promoter, enhancer, and CpG island. DNA and Cell Biol. 18: 17-27.

1999 Lin, C.-S., El-Sakka, A. I., Chui, R. M., Dahiya, R, and Lue, T. F. Effects of diabetes on nitric oxide synthase and growth factor genes and protein expression in an animal model. Intl. J. Impot. Res. 11(3):123-132.

1999 Ruan, H., Wang, J., Hu, L., Lin, C.-S., Lamborn, K. R., and Deen, D. F. Killing of brain tumor cells by hypoxia-responsive element mediated expression of BAX. Neoplasia. 1: 431-437.

2000 Bakircioglu, M. E, Sievert, K.-D., Lau, A, Lin, C.-S., and Lue, T. F. The effect of pregnancy and delivery on the function and ultrastructure of the rat bladder and urethra. BJU Internatl. 85: 350-361.

2000 Lin, C.-S., Lau, A., Yeh, C.-C., Chang, C.-H., and Lue, T. F. Up-regulation of L-plastin gene by testosterone in breast and prostate cancer cells: identification of three cooperative androgen receptor binding sequences. DNA and Cell Biol. 19: 1-7.

2000 Lin, C.-S., Lau, A., Tu, R., and Lue, T. F. Expression of three isoforms of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in human penile cavernosum. Biochem Biophys Res Commun 268: 628-635.

2000 Lin, C.-S., Lau, A., Tu, R., and Lue, T. F. Identification of three alternative first exons and an intronic promoter of human PDE5A gene. Biochem Biophys Res Commun. 268: 596-602.

2000 Bakircioglu, M. E., Hsu, K., El-Sakka, A., Sievert, K.-D., Lin, C.-S., and Lue, T.F. Effect of a Chinese herbal medicine mixture on a rat model of hypercholesterolemic erectile dysfunction. J. Urol. 164(5): 1798-1801.

2001 Lin, C.-S., Chow, S., Lau, A., Tu, R., and Lue, T. F. Identification and regulation of human PDE5A gene promoter. Biochem Biophys Res Commun. 280: 684-692.

2001 Lin, C.-S., Chow, S., Lau, A., Tu, R., and Lue, T. F. Regulation of human PDE5A2 intronic promoter by cAMP and cGMP: identification of a critical Sp1-binding site. Biochem Biophys Res Commun. 280: 693-699.

2001 Lin, C.-S., Liu, X., Spencer, E. M., and Lue, T. F. Insulin-like growth factor-I promotes proliferation and migration of cavernous smooth muscle cells. Biochem Biophys Res Commun. 280: 1307-1315.

2001 Bakircioglu, M. E., Lin, C.-S., Fan, P., Sievert, K.-D., Wefer, J., Kan, Y. W., and Lue, T. F. The effect of adeno-associated virus-mediated brain-derived neurotrophic factor in an animal model for neurogenic impotence. J. Urol. 165: 2103-2109.

2001 Lin, C.-S., Liu, X., Graziottin, T., Resplande, J., and Lue, T. F. Vascular endothelial growth factor promotes proliferation and migration of cavernous smooth muscle cells. J. Urol. 166: 354-360.

2001 Lin, C.-S., Ho, H.-C.,Gholami, S., Chen, K.-C., Jad, A., and Lue, T. F. Gene expression profiling of an arteriogenic impotence model. Biochem Biophys Res Commun. 285: 565-569.

2001 Bakircioglu, M. E., Sievert, K.-D., Nunes, L., Lau, A., Lin, C.-S., and Lue, T. F. Decreased trabecular smooth muscle and caveolin-1 expression in penile tissue of aged rats. J. Urol. 166: 734-738.

2001 Lin, C.-S., and Lue, T. F. Application of molecular biology to impotence research. Molec. Urol. 91-98.

2001 Lin, C.-S., Liu, X., Tu, R., Chow, S., and Lue, T. F. Age-related decrease of protein kinase G activation in vascular smooth muscle cells. Biochem Biophys Res Commun. 287: 244-248.

2001 El-Sakka, A. I.,Yen, T. S., Lin, C.-S., and Lue, T. F. Traumatic arteriogenic erectile dysfunction: a rat model. Intl. J. Impot. Res. 13:162-171.

2002 Lee, M.-C., El-Sakka, A. I., Graziottin, T., Ho, H.-C., Lin, C.-S., and Lue, T. F. The effect of vascular endothelial growth factor on a rat model of traumatic arteriogenic erectile dysfunction. J. Urol. 167: 761-769.

2002 Lin, C.-S., Chow, S., Lau, A., Tu, R., and Lue, T. F. Human PDE5A gene encodes three PDE5 isoforms from two alternate promoters. Intl. J. Impot. Res. 14: 15-24.

2002 Lin, C.-S., Liu, X., Chow, S., and Lue, T. F. Cyclic AMP and cyclic GMP activate protein kinase G in cavernous smooth muscle cells: Old age is a negative factor. BJU International. 89: 576-582.

2002 Resplante, J., Gholami, S. S., Graziottin, T. M., Rogers, R., Lin, C.-S., Leng, W., Lue, T. F. Long-term effect of ovariectomy and simulated birth trauma on the lower urinary tract of female rats. J. Urol. 168: 323-330.

2002 Lin, C.-S., Ho, H.-C., Chen, K.-C., Lin, G., Nunes, L., and Lue, T. F. Intracavernous injection of vascular endothelial growth factor Induces nitric oxide synthase isoforms. BJU International 89: 955-960.

2002 Lin, C.-S., Lin, G., Chen, K.-C., Ho, H.-C., and Lue, T. F. Vascular endothelial growth factor induces IP-10 chemokine expression. Biochem Biophys Res Commun. 292: 79-82.

2002 Lin, C.-S., Lin, G., Wang, Z, Maddah, S. A., and Lue, T. F. Upregulation of monocyte chemoattractant protein 1 and effects of transforming growth factor-beta 1 in Peyronie's disease. Biochem Biophys Res Commun. 295: 1014-1019.

2003 Gholami, G. S., Gonzalez-Cadavid, N. F., Lin, C.-S., Rajfer, J., and Lue, T. F. Peyronie’s disease: A review. J. Urol. 169: 1234-1241.

2003 Rogers, R. S., Graziottin, T. M., Lin, C.-S., Fan, P., Kan, Y. W., and Lue, T. F.

Intracavernosal vascular endothelial growth factor (VEGF) injection and adeno-associated virus-mediated VEGF gene therapy prevent and reverse venogenic erectile dysfunction in rats. Intl. J. Impot. Res. 15: 26-37.

2003 Gholami, G. S., Rogers, R., Chang, J., Ho, H.-C., Grazziottin, T., Lin, C.-S., and Lue, T. F. The effect of VEGF and adeno-associated virus mediated BDNF on neurogenic and vasculogenic erectile dysfunction induced by hyperlipidemia. J. Urol. 169: 1577-1581.

2003 Xin, Z.C., Kim, E.K., Lin, C.-S., Liu, W.J., Tian, L., Yuan, Y.M., and Fu, J. Effects of icariin on cGMP-specific PDE5 and cAMP-specific PDE4 activities. Asian. J. Androl. 5:15-18.

2003 Lin, C.-S., Xin, Z.-C., Lin, G., and Lue, T. F. Phosphodiesterases as therapeutic targets. Urology 61:685-691.

2003 Lin, C.-S., Lin, G., and Lue, T. F. Isolation of two phosphodiesterase 5 isoforms from rat penis. Intl. J. Impot. Res. 15:129-136.

2003 Lin, G., Xin, Z.-C., Lue, T. F., and Lin, C.-S. Up- and down-regulation of phosphodiesterase-5 as related to tachyphylaxis and priapism. J. Urol. In press.

2003 Lin, C.-S. Tissue expression, distribution, and regulation of PDE5. Intl. J. Impot. Res. In press.

2003 Lin, C.-S. and Lue, T. F. Growth factor therapy and neuronal nitric oxide synthase. Intl. J. Impot. Res. In press.

2003 Lin, G., Chen, K.-C., Hsieh, P.-S., and Lue, T. F., and Lin, C.-S. Neurotrophic effects of vascular endothelial growth factor and neurotrophins on cultured major pelvic ganglia. BJU Intl. In press.

2003 Wang, Z., Lin, G., Lue, T. F., and Lin, C.-S. Wogonin Suppresses Cellular Proliferation and Expression of Monocyte Chemoattractant Protein 1 in Peyronie’s Plaque-Derived Cells. BJU Intl. In press.

2003 Lin, C.-S. and Lue, T. F. Cyclic nucleotide signaling in vascular and cavernous smooth muscle: Aging-related changes. In Advances in cell aging and gerontology: volume “Protein phosphorylation in aging” Editor: M.P. Mattson. Publisher: Elsevier. In press.