De Yi  LIU  

Highest degree:        Ph.D (in medicine, University of Melbourne).

Academic position:   Research Fellow (Reproductive Biologist)

 

Present correspondence address:

Dr De Yi Liu, Department of Obstetrics & Gynaecology, University of Melbourne,

Royal Women's Hospital, 132 Grattan Street, Carlton, VIC 3053, Australia.

Phone: 61-3-9344-2042,  Fax: 61-3-9347-1761, Email: dyl@unimelb.edu.au

 

Academic & Employment Record

1977-1980       Diploma, Clinical Pathology and Medical Science, Congqing Medical School, Congqing, Sichuan, China.

1980-1985       Scientist, Andrology Laboratory, Sichuan Family Planning Research Institute (World Health Organisation Cooperation Centre), Chengdu, Sichuan, China. 

1980-1981       Research trainee, Medical Research Centre, Teaching Hospital, Sichuan Medical University, Chengdu, Sichuan, China.

1984-1985       World Health Organization Visiting Scholar, Medical Research Centre, Prince Henry's Hospital, Monash University, Melbourne, Australia.    

1986-1990       Ph.D student. University of Melbourne, Australia.

1991-2004       Research Fellow, University of Melbourne Department of Obstetrics & Gynaecology, Royal Women's Hospital, Carlton,  VIC 3052, Australia.

 

Honorary Professional Positions:

World Health Organization Consultant for “Andrology - Vas Occlusion Workshop” Hanoi, Vietnam, 1993.

Associate Editor of Human Reproduction (2002-2004), an international scientific journal published for the European Society for Human Reproduction and Embryology.

Editorial board member of Reproduction and Contraception (2004, Chinese scientific journal).

 

Reviewer for the following international scientific journals:

Fertility and Sterility, Human Reproduction, Molecular Human Reproduction, International Journal of Andrology (1995-2001), Asia Journal of Andrology, Reproduction and Contraception (Chinese)

 

Professional Memberships

Members of the Australian Society for Reproductive Biology (ASRB) and Fertility Society of Australia (FSA).

 

                                     Publications--(Refereed Scientific Journals)

 

 

1.         Liu DY, Clarke GN and Baker HWG. The effect of serum on motility of human spermatozoa in culture. International Journal of Andrology 9:109-117, 1986

 

2.         Liu DY, Cooper EJ and Baker HWG. Seminal transferrin: an index of Sertoli cell function: Is it of clinical value? Clinical Reproduction and Fertility 4:191-197, 1986

 

3.         Liu DY, Jennings MG and Baker HWG. Correlation between defective motility (asthenospermia) and ATP reactivation of demembranated human spermatozoa. Journal of Andrology 8:349-353, 1987       

 

4.         Liu DY, Elton RA, Johnston WIH and Baker HWG. Spermatozoal nuclear chromatin decondensation in vitro. A test for sperm immaturity. Comparison with results of human in vitro fertilisation. Clinical Reproduction and Fertility 5:191-201, 1987

 

5.         Liu DY, Du Plessis YP, Nayudu PL, Johnston WIH and Baker WHG. The use of in vitro fertilization to evaluate putative tests of human sperm function. Fertility and Sterility 49:272-277, 1988

 

6.         Liu DY and Baker HWG. The proportion of sperm with poor morphology but normal intact acrosomes detected with Pisum Sativum agglutinin correlates with fertilization in vitro. Fertility and Sterility 50:288-293, 1988

 

7.         Liu DY, Lopata A, Johnston WIH and Baker HWG. A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro. Fertility and Sterility 50:782-788, 1988

 

8.                  Liu DY, Lopata A, Johnston WIH and Baker HWG. Human sperm-zona pellucida binding, sperm characteristics and in vitro fertilization. Human Reproduction 4:696-701, 1989.

 

9.                  Liu DY and Baker HWG. Hypoosmotic sperm swelling test as a sperm function test. Fertility and Sterility 51:365-366, 1989.

 

10.              Liu DY, Clarke GN, Lopata A, Johnston WIH and Baker HWG. A sperm-zona pellucida binding test and in vitro fertilization. Fertility and Sterility 52: 281-287,1989.

 

11.              Baker HWG and Liu DY. New tests of human sperm function and in vitro fertilization. Reproduction, Fertility and Development 1:179-182, 1989.

 

12.              Liu DY and Baker HWG. Inducing the human acrosome reaction with a calcium ionophore A23187 decreases sperm-zona pellucida binding with oocytes that failed to fertilize in vitro. Journal of Reproduction and Fertility 89:127-134, 1990. 

 

13.              Liu DY and Baker HWG. Relationships between human sperm acrosin, acrosomes, morphology and in vitro fertilization. Human Reproduction. 5:298-303, 1990.

 

14.              O'Bryan MK, Baker HWG, Saunders JR, Kirszbaum L, Walker ID, Hudson, P, Liu DY, d'Apice AJF and Murphy BF. Human seminal clusterin (SP40,40). Isolation and characterization. Journal of Clinical Investigation. 85:1477-1486, 1990. 

 

15.              Liu DY, Lopata A and Baker HWG. Use of oocytes that failed to be fertilized in vitro to study sperm-oocyte interaction: comparison of sperm-oolemma and sperm-zona pellucida binding, and relationship with results of IVF.  Reproduction, Fertility and Development. 2:641-650, 1990.

 

16.              Liu DY, Clarke GN and Baker HWG. Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies. Fertility and Sterility. 55:440-442, 1991.

 

17.              Liu DY, Clarke GN and Baker HWG. Relationship between sperm motility assessed with the Hamilton-Thorn Motility Analyzer and fertilization rates in vitro. Journal of Andrology, 12:231-239, 1991.

 

18.              Liu DY, Lopata A, Pantke P and Baker HWG. Horse and marmoset sperm bind to the zona pellucida of salt stored human oocytes. Fertility and Sterility, 56:764-767, 1991.

 

19.              Liu DY and Baker HWG. Morphology of spermatozoa bound to the zona pellucida of human oocytes that failed to fertilize in vitro. Journal of Reproduction and Fertility, 94:71-84, 1992.

 

20.              Ng FLH, Liu DY and Baker HWG. Comparison of Percoll, mini-percoll and swim-up methods for sperm preparation from abnormal semen samples. Human Reproduction, 7:261-266, 1992.

 

21.              Liu DY and Baker HWG. Tests of human sperm function and fertilization in vitro. Fertility and Sterility, 58:465-483, 1992.

 

22.              Liu DY and Baker HWG. Sperm nuclear chromatin normality: relationship with sperm morphology, sperm-zona pellucida binding and fertilization rates in vitro. Fertility and Sterility 58:1178-1184, 1992.

 

23.              Liu DY and Baker HWG. Inhibition of acrosin activity with a Trypsin inhibitor blocks human sperm penetration of the zona pellucida. Biology of Reproduction 48:340-348, 1993.

 

24.              Baker HWG and Liu DY, Bourne H and Lopata A. Diagnosis of sperm defects in selecting patients for assisted fertilization.  Human Reproduction, 8:1779-1780, 1993.

 

25.              Liu DY and Baker HWG. Tests of sperm function. Fertility and Sterility, 59:698-699, 1993.

 

26.              Liu DY and Baker HWG. A new test for the assessment of sperm-zona pellucida penetration: relationship with results of other sperm tests and fertilization in vitro. Human Reproduction, 9:489-496, 1994.

 

27.              Liu DY and Baker HWG. Acrosome status and morphology of human sperm bound to the zona pellucida and oolemma determined using oocytes that failed to fertilize in vitro. Human Reproduction, 9:673-679,1994.

 

28.              Liu DY and Baker HWG. Disorderd acrosome reaction of sperm bound to the zona pellucida (ZP). a newly discovered sperm defect causing infertility with reduced sperm-ZP penetration and reduced fertilization in vitro. Human Reproduction, 9:1694-1994, 1994.

 

29.              O'Bryan MK, Murphy BF, Liu DY, Clarke GN and Baker HWG. The use of anticlusterin monoclonal antibodies for the combined assessment of human sperm morphology and acrosome integrity. Human Reproduction, 9:1490-1496, 1994.

 

30.              Clarke,GN, Liu DY and Baker HWG. Immunoinfertility: a case study with implications for immunocontraception. Archives of Andrology, 35:21-27, 1995.

 

31.              Liu DY, Robyn Johnston R and Baker HWG. Ability of sperm to bind to the zona pellucida during oligozoospermia induced with testosterone during a male contraceptive trial. International Journal of Andrology 18 (Suppl 1):39-44, 1995 1995.

 

32.              Johnston RC, Clarke GN, Liu DY, and Baker HWG. Assessment of the Sperm Quality Analyser (SQA). Fertility and Sterility, 63:1071-1076, 1995.

 

33.              Liu DY, Bourne H and Baker HWG. Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm injection in patients with disordered zona pellucida induced acrosome reaction. Fertility and Sterility, 64:116-121, 1995.

 

34.              Bourne H, Liu DY, Clarke GN and Baker HWG.  Normal fertilization and embryo development with an round headed acrosomeless sperm. Fertility and  Sterility, 63:1329-1332, 1995.

 

35.              Pantke P, Hyland J, Galloway DB, Liu DY and Baker HWG. Development of a zona pellucida sperm binding assay for the assessment of stallion fertility. Biology of Reproduction, Monograph 1 (Equine Reproduction VI):681-687, 1995.

 

36.              Bourne H, Richings N, Liu DY, Clarke GN, Harari O and Baker HGW. Sperm preparation for intracytoplasmic injection: methods and relationship to fertilization results. Fertility, Reproduction and Development,7:177-183, 1995.

 

37.              Liu DY and Baker HWG. A simple method for assessment of the human acrosome reaction of spermatozoa bound to the zona pellucida: lack of relatioship with ionophore A23187-induced acrosome reaction. Human Reproduction, 11:551-557, 1996.

 

38.              Liu DY and Baker HWG. Relationship between the zona pellucida (ZP) and ionophore A23187 induced acrosome reaction and the ability of sperm to penetrate the ZP in men with normal sperm-ZP binding. Fertility and Sterility, 66:312-315, 1996.

 

39.              Garrett C, Liu DY and Baker HWG. Selectivity of the human sperm-zona pellucida bining process to sperm head morphometry. Fertility and Sterility, 67, 362-371, 1997.

 

40.              Liu DY, Bourne H and Baker HWG. High fertilization and pregnancy rates after intracytoplasmic sperm injection in patients with disordered zona pellucida-induced acrosome reaction. Fertility and Sterility. 67, 955-958, 1997.

 

41.              Liu DY, Baker HWG, Pears MJ and d Apice AJF. Normal sperm-zona pellucida interaction and fertilization in vitro in a-1,3-galactosyltransferase gene knockout mice. Molecular Human Reproduction. 3, 1015-1016, 1997.

 

42.              Liu DY and Baker HWG. Protein Kinase C plays an important role in human zona pellucida-induced acrosome reaction. Molecular Human Reproduction, 3, 1037-1043, 1997.

 

43.              Liu DY and Baker HWG. Calcium ionophore-induced acrosome reaction correlates with fertilization rates in vitro in patients with teratozoospermic semen. Human Reproduction, 13:905-910, 1998.

 

44.              Liu DY, Martic M, Clarke GN, Dunlop ME and Baker HWG. An important role of actin polymerization in the human zona pellucida-induced acrosome reaction. Molecular Human Reproduction, 5: 941-949, 1999.

 

45.              Liu DY and Baker HWG.  Defective sperm-zona pellucida interaction: a major cause of failure of fertilization in clinical in-vitro fertilization. Human Reproduction, 15: 702-708, 2000.

 

46.              Baker HWG, Liu DY, C Garrett and M Martic The human acrosome reaction. Asian Journal of Andrology, 2: 172-178, 2000.

 

47.              Liu DY, Clarke GN, Martic M, Garrett C and Baker HWG. Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in infertile men with normal semen analysis and normal sperm-ZP binding. Human Reproduction, 6: 1185-1190, 2001.

 

48.              Liu DY, Martic M, Clarke, GN, Grkovic I, Garrett C, Dunlop ME and Baker HWG. An anti-actin monoclonal antibody inhibits the zona pellucida induced acrosome reaction and hyperactivated motility of human sperm. Molecular Human Reproduction, 8, 37-47, 2002.

 

49.              Liu DY, Martic M, Garrett C, Dunlop ME and Baker HWG. Phorbol myristate acetate induces ruffling of the acrosome of human spermatozoa. Fertility and Sterility, 78: 128-136, 2002.

 

50.              Liu DY and Baker HWG. Disordered zona pellucida induced acrosome reaction and failure of in vitro fertilization in patients with unexplained infertility. Fertility and Sterility, 79: 74-80, 2003.

 

51.              Liu, DY and Baker HWG. Frequency of defective sperm-ZP interaction in severely teratospermic infertile men.  Human Reproduction, 18: 802-807, 2003. 

 

52.              Liu DY, Stewart T and Baker HWG. Normal range and variation of the zona pellucida-induced acrosome reaction in fertile men. Fertility and Sterility, 80: 384-389, 2003.

 

53.              Garrett C, Liu DY, Clarke GN, Rushford, DD and Baker, HWG. Automated semen analysis variables : zona pellucida preferred sperm morphometry and straight line velocity, are related to pregnancy rate in subfertile couples. Human Reproduction, 18: 1643-1649, 2003.

 

54.              Liu DY, Garrett C and Baker HWG. Low proportions of spermatozoa can bind to the zona pellucida of human oocytes. Human Reproduction, 18: 2382-2389, 2003.

 

55.              Clarke GN, Liu DY and Baker HWG. Improved sperm cryopreservation after very rapid cooling above the freezing point.  Reproduction, Fertility and Development, 15: 377-381,  2003.

 

56.              Liu, DY and Baker HWG. High frequency of defective sperm-ZP interaction in oligozoospermic infertile men.  Human Reproduction, 19:228-233, 2004.

 

57.              Martic M, Moses E, Adams T, Liu DY, Gook D, Garrett C, Dunlop ME, Baker HWG Recombinant human zona pellucida proteins ZP1, ZP2 and ZP3 co-expressed in a human cell line. Asian Journal of Andrology. 6: 3-13, 2004. 

 

58.              Apedaile AE, Garrett C, Liu DY Clarke GN, Johnston SA and Baker HWG. Flow cytometry and microscopic acridine orange test: relationship with standard semen analysis. Reproductive BioMedicine Online. 8 (4):398-407, 2004.

 

59.              Liu DY, Zhu WJ and Baker HWG. Importance role of assessment of sperm function in clinical management of infertility in IVF/ICSI. Reproduction & Contraception (Chinese) 24 (4): 193-199, 2004.

 

60.              Liu DY, Garrett C and Baker HWG.  Clinical application of sperm-oocyte interaction tests in in vitro fertilization-embryo transfer and intracytoplasmic sperm injection programs. Fertility and Sterility,  In press 2004.

 

61.              Liu DY, Clarke GN and Baker HWG.  Exposing sperm surface actin epitopes during vitro culture relates to sperm functions and capacitation in vitro. Human Reproduction Submitted 2004.

 

62.              Apedaile AE, Garrett C, Liu DY Clarke GN, Bourne H, Johnston SA and Baker HWG.  Does sperm DNA assessment by the flow cytometry acridine orange test predict ART outcome? Reproductive BioMedicine Online. Submitted 2004.

 

 

 

 

                                                  Publications (Book Chapters)

 

 

1             Baker HWG, Ng FLH and Liu DY. Preparation and analysis of semen for IVF/GIFT. In Handbook of In Vitro Fertilization. (1st Edition) Trounson A. O. and Gardner D.K., (eds), CRC press, Boca Raton, pp34-56, 1993.

 

2                         Liu DY and Baker HWG. Tests for human sperm-zona pellucida binding and penetration. In Male Factor in Human Infertility, Tesarik, J. (ed), Ares-Serono Symposia Publications, Frontiers in Endocrinology, Volume 8. Rome, Italy, pp169-185, 1994.

 

3                         Bourne H, Richings N, Liu DY, Clarke GN, Harari O and Baker HGW. Sperm preparation for intracytoplasmic injection: methods and relationship to fertilization results. In Intracytoplasmic Sperm Injection-The Revolution in Male Infertility,. Flaherty S. P. and Matthews C.D. (eds), CSIRO Australia, pp43-49. 1995.

 

4                         Baker HWG and Liu DY. Assessment of nuclear maturity. In Human Spermatozoa in Assisted Reproduction (2nd Edition), Acosta, A.A. and Kruger, T.F. (eds), The Parthenon Publishing Group, New York, pp93-203, 1996.

 

5                         Baker HWG, Liu DY and Bourne H. Assessment of the male and preparation of sperm for ARTs. In Handbook of In Vitro Fertilization. (2nd Edition), Trounson A.O. and Gardner D.K., (eds), CRC press, Boca Raton, pp99-126, 1999.

 

 

Publications--Letters To Editor, Invited

Symposium Abstracts or Papers

 

1.            Liu DY and Baker HWG. Hypoosmotic sperm swelling test as a sperm function test (Letter to the Editor and reply). Fertility and Sterility 51:365-366, 1989.

 

2             Pantke P, Hyland JH, Galloway DB, Liu DY and Baker HWG. Development of zona pellucida sperm binding assay for the assessment of stallion fertility. Australian Equine Veterinarian, 10(2):91, 1992.

 

3             Liu DY and Baker HWG. Relationship between sperm motility assessed with the Hamilton-Thorn Motility Analyzer and fertilization rates in vitro. Fertility Digest 5:8-12, 1992

 

4             Liu DY and Baker HWG. Tests of sperm function. Fertility and Sterility (Letter to Editor and reply) 59:698-699, 1993

 

5             Liu DY and Baker HWG. Morphology of spermatozoa bound to the zona pellucida of human oocytes that failed to fertilize in vitro. Fertility Digest 3: 3-5, 1993.

 

6                         Baker HWG, Liu DY, Bourne H and Lopata A. Diagnosis of sperm defects in selecting patients for assisted fertilization. Fertility Digest 3: 9-11, 1994.

 

7                         Baker HWG and Liu DY and Bourne H. Clinical characteristics of disordered zona pellucida induced acrosome reaction: a newly described cause of infertility. In Human Acrosome reaction (eds) P Fenichel, J Parinaud. Colloque INSERM/John Libbey Eurotext Ltd 1995 Vol 236 pp434-435.

 

8                         Liu DY and Baker HWG. A simple method for assessment of the human acrosome reaction (AR) of spermatozoa bound to the zona pellucida (ZP): lack of relationship with ionophore A23187 induced AR. In Human Acrosome reaction (eds) P Fenichel, J Parinaud. Colloque INSERM/John Libbey Eurotext Ltd 1995 Vol 236 pp432-433.

 

9                         Liu DY and Baker HWG. Defects of sperm-oocyte interaction. In Proceedings of VIth International Congress of Andrology. Salzburg, Austria, 25th to 29th May 1997. Proceeding of Postgratuate Courses pp39-44.

 

10                     Liu DY, Garrett C and Baker HWG. The human acrosome and sperm-oocyte interaction. In proceedings of International Symposium for Young Chinese Reproductive Biologists, Beijing, China, 14-18 October 1997. pp 102-107.

 

11                     Liu DY. Sperm-oocyte interaction tests. In Clinical Application of Advances in Andrology. FSA Satellite Symposium, Australia, 28th October 1998.

 

12                     Liu DY. Application of sperm function tests in IVF/ICSI. In Proceedings of Chongqing ICSI Workshop (Chinese), Chongqing, China, 19-22 November 1999. pp29-35

 

13                     Liu DY. Assessment of sperm for IVF/ICSI, In Proceedings of Chongqing ART Workshop (Chinese), 2-4 September 2002, Chongqing, China, pp95-98.

 

14                     Liu DY and Baker HWG. Evaluation and assessment of semen for IVF/ICSI, In proceedings of  the First Asia Pacific Forum on Andrology, 17-21 Oct 2002, Shanghai, China.

 

15                     Liu DY. Sperm-zona pellucida interaction: a powerful sperm function test for diagnosis and management of unexplained male infertility in clinical IVF/ICSI. In Proceedings of Chongqing ART Workshop (in Chinese), 16-18 December 2003, Chongqing, China, pp46-50.

 

Invited Lectures

1                    Liu DY, Human sperm function. Satellite Symposium on Andrology, 8-10 May 1993, Beijing, China.

2                    Liu DY, Semen analysis and new tests of  human sperm function. WHO sponsored Andrology-Vas Occlusion Techniques Workshop, 23 November to 2 December 1993, Hanoi, Viet Nam.

3                    Liu DY, Tests of human sperm-zona pellucida binding and penetration. International Symposium on Male Factor in Human Infertility, 21-22 April 1994, Paris, France.

4                    Liu DY, Defects of human sperm-oocyte interaction. Postgraduate Course of the VIth International Congress of Andrology, 29-29 May 1997, Salzburg, Austria.

5                    Liu DY, The human acrosome and sperm-oocyte interaction reaction. International Symposium for Young Chinese Reproductive Biologists, 14-18 October 1997, Beijing, China.

6                    Liu DY. Sperm-oocyte interaction tests. In Clinical Application of Advances in Andrology. Fertility Society of Australia (FSA) Satellite Symposium, Hobart, Australia, 28th October 1998.

7                    Liu DY. Clinical application of sperm function tests in IVF/ICSI. Chongqing ICSI Workshop, 19-22 November 1999. Chongqing, China.

8                    Liu DY. Reproductive medical research. Symposium on Establishment of Research and Business Cooperation between Chinese Experts and Oversea Chinese Scholars. 17 to 21 December 2001, Guiling, Guangxi, China.

9                    Liu DY. Implication of sperm function assessment in clinical IVF/ICSI. Chongqing ART workshop, 2-4 September 2002, Chongqing, China.

10                Liu DY. Evaluation and assessment of semen for IVF/ICSI. The First Asia-Pacific Forum on Andrology, 17-21 October 2002, Shanghai, China.

11                Liu DY.  Sperm-zona pellucida interaction: an important sperm function test for diagnosis and management of unexplained male infertility in clinical IVF/ICSI. Chongqing ART workshop, 16-18 December 2003, Chongqing, China

12                Liu DY. Assessment of sperm function for diagnosis and management of male infertility in clinical IVF/ICSI. The 1st International forum on Reproductive Medicine and the 1st Asia Community Genetics Workshop, Shenzhen, China, 10-18, April 2004.

13                Liu DY. Importance of assessment of human sperm function in clinical IVF/ICSI. The 2nd International Congress of Reproductive Medicine, the 1st Affiliated Hospital of Lanzhou Medical University, Lanzhou, China, 8-10 September 2004.

14                Liu DY. Laboratory aspects and clinical application of new sperm function tests. Bangkok Andrology Workshop 2004, Bangkok, Thailand, 19 October 2004.

 

Abstracts Presented At National And International Scientific Meetings

              

1                         Liu DY, Cooper EJ and Baker HWG. Seminal transferrin, an index of Sertoli cell function, possible clinical value. Proceedings of the 17th Annual Conference, Australian Society for Reproductive Biology, Adelaide, Australia, 25-28 August 1985, Abstract 44.

 

2                         Liu DY and Baker HWG. Effect of serum on motility of human spermatozoa in culture. Proceedings of the 4th Annual Scientific Meeting, The Fertility Society of Australia. Melbourne, Australia, 15-16 November 1985, Abstract 34.

 

3                         Liu DY, Jennings MG and Baker HWG. Reactivation of flagellar motion of detergent treated human spermatozoa by adenosine triphosphate. Proceedings of the 18th Annual Conference, Australian Society for Reproductive Biology, Brisbane, Australia, 31st August to 3rd September 1987, Abstract 122.

 

4                         Liu DY, Du Plessis Y, Penny LN and Baker HWG. The use of IVF to evaluate putative tests of human sperm function. Proceedings of the 5th Annual Scientific Meeting, The Fertility Society of Australia. Adelaide, Australia, 2-6 December 1986, Abstract 029.

 

5                         Liu DY, Lopata A, Bourne H and Baker HWG. Human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro.  Proceedings of the 19th Annual Conference, Australian Society for Reproductive Biology, Sydney, Australia, 24-26 August 1987, Abstract 1.

 

6                         Liu DY, Du Plessis Y and Baker HWG. Nuclear chromatin decondensation; a test for sperm immaturity, is it of clinical value?  Proceedings of the 19th Annual Conference, Australian Society for Reproductive Biology, Sydney, Australia, 24-26 August 1987, Abstract 94.

 

7                         Rowsell AL, Svalbe ID, Osborne CF, Clarke GN, Liu DY and Baker HWG. Objective assessment of sperm morphology using image analysis. Proceedings of the 19th Annual Conference, Australian Society for Reproductive Biology, Sydney, Australia, 24-26 August 1987, Abstract 124.

 

8                         Liu DY and Baker HWG. Evaluation of human sperm acrosome morphology using a fluorescence conjugated lectin--Pisum Sativum agglutinin (PSA). Proceedings of the 6th Annual Scientific Meeting, The Fertility Society of Australia. Sydney, Australia, 11-14 November 1987, Abstract 071.

 

9                         Gook DA, Liu DY and Wiltshire CJ. Glycosaminoglycans from human follicular fluid enhance the acrosome reaction in human spermatozoa. Proceedings of the 6th Annual Scientific Meeting, The Fertility Society of Australia. Sydney, Australia, 11-14 November 1987, Abstract 072. 

 

10                     Liu DY, Johnston WIH and Baker HWG. Contribution of sperm abnormalities to failure of in vitro fertilization. Proceedings of the 6th Annual Scientific Meeting, The Fertility Society of Australia. Sydney, Australia, 11-14 November 1987, Abstract 17. 

 

11                     Liu DY and Baker HWG. Correlation between sperm characteristics, zona binding and human in vitro fertilization. Proceedings of the 20th Annual Conference, Australian Society for Reproductive Biology, Newcastle, Australia, 29th August to 1st September 1988, Abstract 39.

 

12                     Liu DY and Baker HWG. Correlation between sperm morphology, acrosome and acrosin and fertilization in vitro. Proceedings of the 20th Annual Conference, Australian Society for Reproductive Biology, Newcastle, Australia, 29th August to 1st September 1988, Abstract 40.

 

13                     Liu DY, Clarke GN, Lopata A, Johnston WIH and Baker HWG. The sperm-zona pellucida binding test is useful for predicating the ability of sperm to fertilize in vitro. Proceedings of the 7th Annual Scientific Meeting, The Fertility Society of Australia, Newcastle, Australia, 29th to 1st September 1988, Abstract 008.

 

14                     Chen ZW, Zhou ZG, Liu DY, Howard EJ and Baker HWG. Human acrosome assessment with fluorescein labelled Pisum Sativum agglutinin (PSA): relationship with other semen characteristics. Proceedings of the 7th Annual Scientific Meeting, The Fertility Society of Australia, Newcastle, Australia, 29th to 1st September 1988, Abstract 035.

 

15                     Liu DY and Baker HWG. Use of oocytes that failed to fertilize in vitro to study human sperm-egg interaction: comparison of sperm-oolemma and sperm-zona pellucida binding and relationship with results of IVF. (Abstr) pp 146, IV International Congress of Andrology Miniposters, Florence, Italy, May 14-18, 1989. (ed) M Serio, Published by Ares-Serono Symposia, Via Raenna, 8-Roma, Italy.

 

16                     Liu DY, Clarke GN and Baker HWG. Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies. Proceedings of the 21st Annual Conference, Australian Society for Reproductive Biology, Monash University, Melbourne, Australia, 25th to 27th September 1989, Abstract 66.

 

17                     Liu DY and Baker HWG. Inducing the human acrosome reaction with an ionophore A23187 decreases sperm-zona pellucida binding and increases sperm-oolemma binding with oocytes that failed to fertilize in vitro.  Proceedings of the 21st Annual Conference, Australian Society for Reproductive Biology, Monash University, Melbourne, Australia, 25th to 27th September 1989, Abstract 113.

 

18                     Rowsell AL, Liu DY, Clarke GN and Baker HWG. Objective image analysis of human sperm morphology related to fertilization rates in vitro. Proceedings of the 21st Annual Conference, Australian Society for Reproductive Biology, Monash University, Melbourne, Australia, 25th to 27th September 1989, Abstract 124.

 

19                     Liu DY, Gary NC and Baker HWG. Sperm velocity and morphology in semen correlates with fertilization rates in vitro. Proceedings of the 8th Annual Scientific Meeting, The Fertility Society of Australia, Canberra, Australia, 29th November to 2nd December 1989, Abstract 104.

 

20                     Liu DY, Rowsell AL and Baker HWG. Evaluation of morphology status of sperm bound to the zona pellucida (ZP) of human oocytes that failed to fertilize in vitro. Proceedings of the 8th Annual Scientific Meeting, The Fertility Society of Australia, Canberra, Australia, 29th November to 2nd December 1989, Abstract 105.

 

21                     Fox ACK, Liu DY and Baker HWG. Sperm morphology in relation to sperm counts in normal men with testosterone induced oligospermia. Proceedings of the 8th Annual Scientific Meeting, The Fertility Society of Australia, Canberra, Australia, 29th November to 2nd December 1989, Abstract 101.

 

22                     Rowsell AL, Liu DY, Gary NC and Baker HWG. Automated image analysis and it's application to sperm morphology. Proceedings of the 8th Annual Scientific Meeting, The Fertility Society of Australia, Canberra, Australia, 29th November to 2nd December 1989, Abstract 54.

 

23                     O'Bryan MK, Baker HWG, Saunders JR, Kirszbaum L, Walker ID, Liu DY and Murphy BF: Human seminal SP-40,40 (Clusterin). Isolation and characterization. Proceedings of the 8th Annual Scientific Meeting, The Fertility Society of Australia, Canberra, Australia, 29th November to 2nd December 1989, Abstract 102.

 

24                     Johnston I, McBain J, Liu DY, Odawara Y, Lopata A, du Plessis Y, Jones G, Clarke GN, Hill P and Baker HWG.  Male factor infertility, selection and results for assisted procreation. Proc 13Th IFFS World Congress on Fertility and Sterility. 1-6 October 1989.  Marrakesh, Morocco.

 

25                     Ng FLH, Liu DY and Baker HWG. Induction of the acrosome reaction by human follicular fluid. Proceedings of 22nd Annual Conference, Australia Society for Reproductive Biology, University of Western Australia, Perth, Australia, 23rd to 26th September 1990, Abstract 30.

 

26                     Liu DY, Rowsell AL and Baker HWG. Objective measurement of head dimension of sperm bound to the zona pellucida of human oocytes. Proceedings of 22nd Annual Conference, Australia Society for Reproductive Biology, University of Western Australia, Perth, Australia, 23rd to 26th September 1990, Abstract 28.

 

27                     Liu DY, Liu ML and Baker HWG. Induction of the acrosome reaction of human sperm with poor morphology by heparin and high concentration calcium. Proceedings of the 9th Annual Scientific Meeting, The Fertility Society of Australia, Perth, 26th to 29th, September 1990, Abstract 54.

 

28                     Liu ML, Liu DY, Clarke GN and Baker HWG. Increased proportion of motile sperm with normal morphology after sperm swim-up in seminal plasma supplemented medium. Proceedings of the 9th Annual Scientific Meeting, The Fertility Society of Australia, Perth, 26th to 29th, September 1990, Abstract 13.

 

29                     Ng FLH, Liu DY and Baker HWG. Comparison of percoll, minpercoll and swim-up methods for sperm preparation of poor semen samples. Proceedings of the 9th Annual Scientific Meeting, The Fertility Society of Australia, Perth, 26th to 29th, September 1990, Abstract 13.

 

30                     Baker HWG, Liu DY and Speirs AL. Prediction of fertilization rates in IVF from semen analysis results and diagnostic category. Proceedings of the 9th Annual Scientific Meeting, The Fertility Society of Australia, Perth, 26th to 29th, September 1990, Abstract 51.

 

31                     Bourne H, Hale L, Vassiliadis, Liu DY, Lopata and Baker HWG. Viability of embryos resulting from fertilization of zona opened oocytes. Proceedings of the 9th Annual Scientific Meeting, The Fertility Society of Australia, Perth, 26th to 29th, September 1990, Abstract 20.

 

32                     Liu DY, Lopata A, Pantke P and Baker HWG. Horse and marmoset monkey sperm bind to the zona pellucida of salt stored human oocytes. Proceedings of 23rd Annual Conference, Australia Society for Reproductive Biology, University of Sydney, Sydney, Australia, 30th September to 2 October 1991, Abstract 116.

 

33                     Liu DY and Baker HWG. Inhibition of acrosin activity with trypsin inhibitor blocks human sperm penetration of the zona pellucida. Proceedings of the 10th Annual Scientific Meeting, The Fertility Society of Australia, Lorne, Victoria, 18th to 22nd, November 1991, Abstract 22. 

 

34                     Liu DY and Baker HWG. Human sperm morphology, nuclear maturity, DNA normality, sperm-zona binding and failure of fertilization in vitro. Proceedings of the 10th Annual Scientific Meeting, The Fertility Society of Australia, Lorne, Victoria, 18th to 22nd, November 1991, Abstract 41.

 

35                     Liu DY and Baker HWG. Acrosome status and morphology of sperm bound to the zona pellucida and oolemma using human oocytes that failed to fertilize in vitro. Proceedings of 24th Annual Conference, Australia Society for Reproductive Biology, University of Adelaide, Adelaide, Australia, 28th to 30th September 1992, Abstract 97.

 

36                     Liu DY and Baker HWG. Relationship between human sperm abnormalities, defective sperm-zona pellucida interactions and failure of fertilization in vitro. Proceedings of the 11th Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia, 2nd to 5th, December 1992, Abstract 137.

 

37                     Bourne H, Liu DY, Baker HWG and Johnston WIH. relationship between sperm characteristics and fertilization following microinjection.  Proceedings of the 11th Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia, 2nd to 5th, December 1992, Abstract 131.

 

38                     Vassiliadis A, Jones G, Johnston WIH, Speirs AL, Liu DY and Baker HWG. Poor fertilization rates in IVF.  Proceedings of the 11th Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia, 2nd to 5th, December 1992, Abstract 139.

 

39                     Clayton-Smythe M, Bourne H, Liu DY, Speirs AL, Baker HWG and Johnston WIH. Treatment of teratozoospermia in IVF: Effect of increasing numbers of sperm inseminated.  Proceedings of the 11th Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia, 2nd to 5th, December 1992, Abstract 146.

 

40                     Liu DY and Baker HWG. New tests of human sperm function and in vitro fertilization (IVF). The Proceedings of the Vth International Congress of Andrology, Tokyo, Japan, 2nd to 6th, May 1993, Abstract, P67.

 

41                     Baker HWG and Liu DY. Relationship between human sperm abnormalities, defective sperm-zona pellucida interactions and failure of fertilization in vitro.  North American Testis Workshop, April, Tampa, Florida, USA, Abstract, 1993.

 

42                     Liu DY and Baker HWG. Semen analysis and tests of human sperm function. Proceedings of Satellite Symposium on Andrology to Vth international Congress of Andrology. Beijing, China, 8th to 10th May, 1993. Review lecture, P128. 

 

43                     Liu DY and Baker HWG. Disordered acrosome reaction of sperm bound to the zona pellucida (ZP): A newly discovered sperm defect causing reduced sperm-ZP penetration and fertilization in vitro with otherwise normal semen. Proceedings of the 12th Annual Scientific Meeting, The Fertility Society of Australia, Sydney, 2nd to 6th, November 1993, Abstract 59.

 

44                     Liu ML, Liu DY and Baker HWG. Head dimensions of morphologically normal sperm bound to the zona pellucida of human oocytes and in insemination medium: relationship with results of IVF. Proceedings of the 12th Annual Scientific Meeting, The Fertility Society of Australia, Sydney, 2nd to 6th, November 1993, Abstract 75.

 

45                     Baker HWG, O'Bryan MK, Liu DY, and Murphy BF. Clusterin- A Marker of sperm quality. Proceedings of the 12th Annual Scientific Meeting, The Fertility Society of Australia, Sydney, 2nd to 6th, November 1993, Abstract 31.

 

46                     Johnson R, Liu DY, Clarke GN and Baker HWG. Assessment of sperm quality analyser (SQA). Proceedings of the 12th Annual Scientific Meeting, The Fertility Society of Australia, Sydney, 2nd to 6th, November 1993, Abstract 31.

 

47                     Fleishman GC, Liu DY, Clarke GN, Murphy BF, Polihronis M and Baker HWG. Initial characterization of a monoclonal antibody to human sperm. Proceedings of the 12th Annual Scientific Meeting, The Fertility Society of Australia, Sydney, 2nd to 6th, November 1993, Abstract 71.

 

48                     Liu DY, Bourne H and Baker HWG. Fertilization and pregnancy with acrosome intact sperm by ICSI in patients with disordered zona pellucida (ZP) induced acrosome reaction causing failure of ZP penetration and fertilization in IVF. Proceedings of the 13th Annual Scientific Meeting, The Fertility Society of Australia, Brisbane, 3rd to 7th October 1994, Abstract 115.

 

49                     Liu DY and Baker HWG. Human acrosome and sperm-oocyte interactions in vitro. Proceedings of 7th International Symposium on Spermatology, Cairns, North Queensland, Australia, 9th to 14th October 1994, Abstract 7.18.

 

50                     Liu DY and Baker HWG. A simple method for assessment of the human acrosome reaction (AR) of spermatozoa bound to the zona pellucida: lack of relationship with ionophore A23187 induced AR. Proceedings of the 27th Annual Conference, Australia Society for Reproductive Biology, The World Congress Center, Melbourne, Australia, 25th to 27th September 1995, Abstract 15.

 

51                     Baker HWG, Liu DY and Bourne H. Clinical characteristics of disordered zona pellucida induced acrosome reaction: a newly described cause of infertility. Proceedings of the 27th Annual Conference, Australia Society for Reproductive Biology, The World Congress Center, Melbourne, Australia, 25th to 27th September 1995, Abstract 109.

 

52                     Fleishman GC, Liu DY Clarke GN, Murphy BF and Baker HWG. Characterisation of a monoclonal antibody to the human acrosome. Proceedings of the 27th Annual Conference, Australia Society for Reproductive Biology, The World Congress Center, Melbourne, Australia, 25th to 27th September 1995, Abstract 17.

 

53                     Liu DY and Baker HWG. Human zona pellucida (ZP) induced acrosome reaction correlates with the ability of sperm to penetrate the ZP. Proceedings of the 14th Annual Scientific Meeting, The Fertility Society of Australia, Melbourne, 19-25 November 1995, Abstract 39.

 

54                     Garrett C, Liu DY and Baker HWG. The role of sperm head densiomethry in zona binding selectivity and prediction of IVF rates. Proceedings of the 14th Annual Scientific Meeting, The Fertility Society of Australia, Melbourne, 19-25 November 1995, Abstract 84.

 

55                     Liu DY and Baker HWG. A simple method for assessment of the human acrosome reaction (AR) of spermatozoa bound to the zona pellucida: lack of relationship with ionophore A23187 induced AR. International symposium on human sperm acrosome reaction. Collioure, France 7-9 September 1995, Human Sperm Acrosome Reaction (eds) Fenichel P, Parinaud J Colloque INSERM/John Libbey Eurotext Ltd, 1995 Vol 236, pp 432-433.

 

56                     Baker HWG, Liu DY and Bourne H. Clinical characteristics of disordered zona pellucida induced acrosome reaction: a newly described cause of infertility. Human Sperm Acrosome Reaction (eds) Fenichel P, Parinaud J Colloque INSERM/John Libbey Eurotext Ltd, 1995 Vol 236, pp 434-435.

 

57                     Liu DY and Baker HWG. Protein kinase C plays a major role in signal transduction in the human zona pellucida-induced acrosome reaction. Proceedings of the 15th Annual Scientific Meeting, The Fertility Society of Australia, Queenstown, New Zealand, 9-14 September 1996, Abstract 105.

 

58                     Baker HWG, Liu DY and Bourne H. ICSI is effective treatment for patients with failure of IVF caused by disordered zona pellucida induced acrosome reaction.  Proceedings of the 15th Annual Scientific Meeting, The Fertility Society of Australia, Queenstown, New Zealand, 9-14 September 1996, Abstract 37.

 

59                     Liu DY and Baker HWG. Phorbol ester augments the human zona pellucida-induced acrosome reaction. VIth International Congress of Andrology, Salzburg, Austria 25-29 May 1997. International Journal of Andrology, supplement 1. Abstract 190.

 

60                     Baker HWG, Bourne H and Liu DY. Clinical characteristics and results of ICSI inpatients with disordered zona pellucida induced acrosome reaction. VIth International Congress of Andrology, Salzburg, Austria 25-29 May 1997.  International Journal of Andrology, supplement 1. Abstract 189.

 

61                     Liu DY and Baker HWG. Defects of sperm-zona pellucida binding and penetration: Major cause for failure of fertilization in vitro. Proceedings of the 16th Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia, 2-4 December 1997, Abstract 30

 

62                     Baker HWG, Clarke GN and Liu DY. Frequency of disordered zona pellucida-induced acrosome reaction in normozoospermic men. Proceedings of the 16th Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia, 2-4 December 1997, Abstract 30

 

63                     Liu DY, Martic M, Clarke GN, Dunlop ME and Baker HWG. Actin plays an important role in human hyperactivation and zona pellucida-induced acrosome reaction. Proceedings of the 17th Annual Scientific Meeting, The Fertility Society of Australia, Hobart, Tasmania, 28-31, October 1998, pp 50.

 

64                     Garrett C, Liu DY, Rushford DD, McDonald JL and Baker HWG. Relationships between sperm morphometry and the suppression response of human spermatogenesis to testosterone (T)-implant.  Proceedings of the 17th Annual Scientific Meeting, The Fertility Society of Australia, Hobart, Tasmania, 28-31, October 1998, pp 88.

 

65                     Liu DY and Baker HWG. An importune role of protein kinase C in the regulation of the human zona pellucida-induced acrosome reaction. Proceedings of 11th World Congress on In Vitro Fertilization and Human Reproductive Genetics, Sydney, Australia, 9-14 May 1999. pp 95.

 

66                     Martic M, Fleishman GC, Liu DY, Dunlop ME and Baker HWG. Antibodies to intracellular proteins affect the human zona pellucida induced acrosome reaction-physiological effect or artifact? Proceedings of 11th World Congress on In Vitro Fertilization and Human Reproductive Genetics, Sydney, Australia, 9-14 May 1999. pp 97. 

 

67                     Baker HWG and Liu DY. Diagnosis of disordered zona pellucida induced acrosome reaction in pre-IVF patients with normal semen analysis. Proceedings of 11th World Congress on In Vitro Fertilization and Human Reproductive Genetics, Sydney, Australia, 9-14 May 1999. pp 94.

 

68                     Garrett C, Liu DY and Baker HWG. Toward an objective standard assessment of human sperm morphology.  Proceedings of 11th World Congress on In Vitro Fertilization and Human Reproductive Genetics, Sydney, Australia, 9-14 May 1999. pp94.

 

69                     Liu DY, Martic M, Clarke GN, Grkovic I, Garrett C, Dunlop ME and Baker HWG. Anti-actin monoclonal antibody inhibits hyperactivation and the zona pellucida induced acrosome reaction of human sperm. Proceedings of the Annual Scientific Meeting, The Fertility Society of Australia and Australian Society for Reproductive Biology, Canberra, 26-30, April 2000, pp 53.

 

70                     Martic M, Liu DY, Dunlop ME and Baker HWG. SNARE proteins, Syntaxin and Munc-18 are present in human sperm. Proceedings of the Annual Scientific Meeting, The Fertility Society of Australia and Australian Society for Reproductive Biology, Canberra, 26-30, April 2000, pp 57.

 

71                     Clarke GN, Liu DY Martic M, O’Bryan M and Baker HWG. Sperm autoimmunity in a patient with severe asthenospermia and short sperm tails. Proceedings of the Annual Scientific Meeting, The Fertility Society of Australia and Australian Society for Reproductive Biology, Canberra, 26-30, April 2000, pp 176.

 

72                     Martic M, Liu DY, Dunlop ME, and Baker HWG. SNARE proteins, Syntaxin and Munc-18 are presented in human spermatozoa. The 16th Annual Meeting of European Society of Human Reproduction and Embryology, Bologna, Italy, June 25-28, 2000, Human Reproduction 15: Abstract Book 1, June 2000, pp83, O-206. 

 

73                     Liu DY, Jobson S, Martic M, Garrett C, Dunlop ME and Baker HWG. Role of protein kinase C in the human zona pellucida-induced acrosome reaction and ruffling of the acrosome. The 16th Annual Meeting of European Society of Human Reproduction and Embryology, Bologna, Italy, June 25-28, 2000, Human Reproduction 15: Abstract Book 1, June 2000, pp112.

 

74                     Baker HWG and Liu DY. Pre-IVF diagnosis of disordered zona pellucida induced acrosome reaction in unexplained infertility with normal semen analysis. The International Congress of Endocrinology, Sydney, October 2000.

 

75                     Martic M, Moss E, Adams T, Liu DY, Dunlop ME and Baker HWG. Recombinant human zona pellucida ZP1, ZP2, and ZP3 co-expressed in a human cell line. The International Congress of Endocrinology, Sydney, Australia, October 2000.

 

76                     Baker HWG, Liu DY, Martic, M, Jobson S, Grkovic I, Garrett, C and Dunlop ME. Ruffling of the acrosome of human sperm. The 7th International Congress of Andrology, Montreal, Canada , June 15th-19th 2001. 

 

77                     Liu DY and Baker HWG. Disordered acrosome reaction and unexplained infertility. Proceedings of the 17th World Congress on Fertility and Sterility, Melbourne, Australian, November 25th to 30th 2001. P87.

 

78                     Garrett C, Apedaile A, Liu DY, Clarke G and Baker HWG. Correlation between automated semen analysis techniques. Proceedings of the 17th World Congress on Fertility and Sterility, Melbourne, Australian, November 25th to 30th 2001. P12.

 

79                     Stewart TM, Liu DY, Liu ML, Garrett C, Baker HWG, Brown E and Venn A. Assessment of bias in surveillance of male fertility. Proceedings of the 17th World Congress on Fertility and Sterility, Melbourne, Australian, November 25th to 30th 2001. P133.

 

80                     Apedaile A, Garrett C, Liu DY, Clarke G, Bourne H and Baker HWG. Sperm chromatin structure assay (SCSA) in ART. Proceedings of the 17th World Congress on Fertility and Sterility, Melbourne, Australian, November 25th to 30th 2001. P130.

 

81                     Liu DY, Garrett C and Baker HWG. Sperm zona binding capacity in fertile and subfertile men. Proceedings of the 21st Annual Scientific Meeting, The Fertility Society of Australia, Gold Coast, Queensland, 23-26, October 2002, pp 61.

 

82                     Stewart TM, Liu DY, Liu ML, Garrett C, Baker HWG, Brown E and Venn A. Surveillance of human fertility and semen quality in an Australian Population. Proceedings of the 21st Annual Scientific Meeting, The Fertility Society of Australia, Gold Coast, Queensland, 23-26, October 2002, pp 55

 

83                     Liu DY, Garrett C and Baker HWG. Defective sperm-zona interaction in subfertile men. Proceedings of the 22nd Annual Scientific Meeting, The Fertility Society of Australia, Perth, West Australia, 1-5, November 2003, pp35.

 

84                     Garrett C, Liu DY and Baker HWG. Automation improves prognosis of pregnancy in the subfertile. Proceedings of the 22nd Annual Scientific Meeting, The Fertility Society of Australia, Perth, West Australia, 1-5, November 2003, pp32.

 

85                     Liu DY, Clarke GN and Baker HWG. Exposure of actin on the surface of human sperm heads during culture correlates with sperm morphology and zona pellucida binding ability. Proceedings of the 23 Annual Scientific Meeting, The Fertility Society of Australia, Adelaide, South Australia,  10-13, October 2004, pp33.