Revised January, 2005

                                                               

BARRY R. ZIRKIN

 

                                                                             

I. A       PERSONAL DATA

                                   Business Address

            Department of Biochemistry and Molecular Biology

            Division of Reproductive Biology

            The Johns Hopkins University School of Public Health 

            Baltimore, MD 21205

            Ph:  410-955-7827

            Fax:  410-614-2356

            e-mail: brzirkin@jhsph.edu

 

I. B       EDUCATION and TRAINING

Year                 Degree                                    Institution and Field

1963                BA, Biology                  State University of New York, Binghamton

1965                MS, Biology                  University of Rochester

1969                PhD, Cell Biology         University of Rochester

1969-71           Postdoctoral Fellow,    University of California, Davis

  Cell Biology

 

I. C       PROFESSIONAL EXPERIENCE

 

Assistant Professor, 1971-74, Department of Biology, Illinois Institute of Technology,

Chicago, IL

Assistant Professor, 1974-76, Department of Population Dynamics, Johns Hopkins

            University, School of Public Health

Associate Professor, 1976-81, Department of Population Dynamics, Johns Hopkins

University, School of Public Health

Professor, 1981-present, The Johns Hopkins University, School of Public Health.

(Department of Population Dynamics, 1981-1998; Department of Biochemistry and Molecular Biology, 1998-present) 

Head, Division of Reproductive Biology, Johns Hopkins University, School of Public Health, 1984- present.

Professor, Joint Appointment, Department of Gynecology and Obstetrics, Johns Hopkins      University, School of Medicine, 1991- present.

 

I. D       PROFESSIONAL ACTIVITIES

 

Society Memberships and Leadership Positions

American Society for Cell Biology

Chairman, Congressional Liaison Committee, 1993-1998

Endocrine Society       

Society for the Study of Reproduction

Member, Committee on Awards, 1976

Member, Membership Committee, 1980-1981

Assistant Editor, Biology of Reproduction, 1981-1985

Treasurer, 1985-1988

Member, Fiscal Committee, 1988-1990

Chair, Publications Committee, 1989-1990

Member, Nominating Committee, 1992-1993

Chair, Program Committee, 1994-1995

Member, Board of Directors, 1995-1997

Member, Publications Committee, 1998-2000

Representative, Committee on Science Policy, FASEB, 1999-2001

American Society of Andrology

Chair, Publications Committee, 1989-1990

Member, Publications Committee, 1991-1993

Chair, Program Committee, 1993-1994      

Member, Executive Council, 1997-2000

Member, Program Committee, 1999-2000

Vice-President, 2000-2001     

President, 2001-2002

Past President, 2002-2003

Chair, Development Committee: Basic Science, 2003-2004

North American Testis Workshop

            Chairman, Abstract Review Committee, 1988

Member, Program Committee, 1993-1997

Chair, Program Committee, 1997

Chair, Executive Scientific Committee, 1997-1998

Member, Executive Scientific Committee, 1998-present

National Institute of Child Health and Human Development, NIH; and Marine Biological

    Laboratory, Woods Hole

Program Chair, Frontiers in Reproduction Research Symposium, 1998

 

Participation on Advisory Panels

Member, NIH Clinical 3 Study Section, 1980-1984

Reviewer, Ford Foundation Research Grant Proposals, 1981-1983

Member, NIH Reproductive Biology Study Section, 1984-1988

Member, MARC (Minority Access to Research Careers) Review Committee, HHS, 1990

Member, NIH Population Center Grant Reviews, 1990-1993.

Member, Special Review Committee, NIDDK (Regulation of Prostate Growth: Basic and

Clinical Studies), 1993

Reviewer, NICHD Scientific Review Program, 1992

Reviewer, Human Genetics Branch, NICHD, 1993

Reviewer, Reproductive Toxicity Risk Assessment Guidelines, U.S.E.P.A., 1994

Member, Scientific Review, Idiopathic Male Infertility, NICHD, NIH, 1994

Member, Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) Scientific Advisory Panel,

1996

Member, Endocrinology and Reproductive Sciences Special Emphasis Panel, NIH, 1997

Member, Merit Review Board for Endocrinology, Department of Veterans Affairs, 1994-1998

Member, Review of Scientific Personnel, National Institute on Environmental Health Sciences,  

1998

Member, Endocrinology of Aging Advisory Committee, National Institute on Aging, 1992-2000

Chair, NIH Review Committee for Small Business Awards, 1999

Member, National Research Council Committee on Toxicology, Subcommittee on Reproductive

and Developmental Toxicants, 1997-2000

Member, NIH Center for Scientific Review Special Emphasis Panel, 2002

Member, Interagency Coordinating Committee for the Validation of Alternative Methods

            (ICCVAM) and the National Toxicology Program Interagency Center for the Evaluation          of Alternative Toxicological Methods (NICEATM) Endocrine Disruptor Peer Review Panel,  2002.

Chair, NIH Special Study Section, "Male Site, Contraceptive Clinical Trials."  Male

Contraceptive Network, 2003. 

Member, NIH Special Review Study, Section, ZRG1 SSS T:10B: Small Business:

Endocrinology, Metabolism, Nutrition & Reproductive Sciences, NIH, 2003.

Ad Hoc Member, NIH Reproductive Biology Study Section, 2003.

 

Program or Project Development

Director, Training Program in the Cellular and Molecular Bases of Aging, 1996-2002

Director, Program Project on Aging of the Male Reproductive Tract, 1997-2002

Director and P.I.,, Specialized Cooperative Center in Reproduction Research, 1998-2003.

Director and P.I., Training Program in the Reproductive Sciences, 1995-present

Director and P.I., Specialized Cooperative Center in Reproduction Research, 2004-  (pending)

 

Consultancies

University of Pennsylvania Coordinated Program in Reproductive Biology, 1986-1987; 1990-           1993

Fonds Pour La Formation De Chercheurs et L'Aide a la Recherche, University of Montreal, 1989

Ares-Serono, Male Infertility, 1991, 1992

Washington National Zoo, 1990-1995

Environmental Studies and Toxicology, The National Academies, 1997-2000

Chairman, Scientific Advisory Panel, Endocrine Disruptor International Cooperative Research Center (EDICOR), 2000-2002

External Advisor, Washington State University Center for Reproductive Sciences, 2000-

External Advisor, Washington National Zoo, 2004-present

External Advisor, McGill University Center on Healthy Gametes, 2004.

 

I.E        EDITORIAL ACTIVITIES

 

Editorial Board Membership

Assistant Editor, Biology of Reproduction, 1981-1985

Editorial Board, Endocrinology, 1989-1993

Consulting Editor, Biology of Reproduction, 1991-1995

Editorial Board, Journal of Andrology, 1992-1996

Editorial Board, Endocrine Journal, 1992-1996.

Editorial Board, Endocrine, 2000-2004.

Editorial Board, Archives of Medical Research, 1994-present

Editorial Board, Reproductive Toxicology, 2001-present

Editorial Board, Endocrinology, 2005-present

 

I. F       HONORS and AWARDS

Elected Member, Delta Omega Alpha Chapter (Honorary Public Health Society), 1987

George E. Owen Teaching Award, Johns Hopkins University School of  Arts and Sciences, 1996

Mentorship Award, Johns Hopkins School of Public Health, 1997

Distinguished Alumnus, State University of New York, Binghamton, 1998

Golden Alumnus, State University of New York, Binghamton, 2000

 

I.G       PUBLICATIONS

 

Books

1. Whitcomb, R.W. and Zirkin, B.R. (eds.) Understanding Male Infertility: Basic and Clinical Approaches, Raven Press, New York, 1993.

2. Zirkin, B.R. (ed.), Germ Cell Development, Division, Disruption and Death, Serono Symposia USA, Springer-Verlag, New York, 1998.

 

Chapters

1. Hayat, M.A. and Zirkin, B.R. 1973.  Critical point drying method.  In: Principles and Techniques of Electron Microscopy, Vol. III (ed. M.A. Hayat), pp. 297-313. Van

Nostrand Reinhold, New York.

2. Ewing, L.L., Cochran, R.C., Zirkin, B.R. and Chubb, C.E. 1980.  Steroid synthesis and secretion by in vitro perfused rat, rabbit, dog, guinea pig and hamster testes.  In: Testicular Development, Structure, and Function. A. Steinberger and E. Steinberger, eds. pp. 117-127. Raven Press, New York.

3. Ewing, L.L., Davis, J.C. and Zirkin, B.R. 1980  Regulation of testicular function: A spatial

and temporal view. Int. Rev. Physiol. 22: 41-115.

4. Zirkin, B.R., Dykman, D.D., Kromann, N., Cochran, R.C. and Ewing, L.L. 1982.  Inhibition

and recovery of testosterone secretion in rats are tightly coupled to quantitative

changes in Leydig cell smooth endoplasmic reticulum.  Annals N.Y. Acad. Sci. 383:17-28.

5. Zirkin, B.R., Soucek, D.A. and Chang, T.S.K. 1982.  Sperm nuclear packing and regulation

during spermatogenesis and fertilization. Johns Hopkins Med. J. 151:101-112.

6. Zirkin, B.R. 1982.  Potential methods for examining sperm chromosomes.  In: Prospects for

Sexing Mammalian Sperm (eds. Amann, R.P. and Seidel, G.E.), p. 248.  Colorado Assoc. Univ. Press, Boulder.

7. Zirkin, B.R. 1982.  Spermatozoal DNA and protamine.  In: Prospects for Sexing Mammalian

Sperm (eds. Amann, R. P. and Seidel, G.E.), pp. 61-62.  Colorado Assoc. Univ. Press, Boulder.

8. Ewing, L.L. and Zirkin, B.R. 1983.  Leydig cell structure and steroidogenic function. Recent

Progr. Hormone Res. 39:599-635.

9. Ewing, L.L., Berry S.J., Juniewicz, P.E., Zirkin, B.R. and Coffey, D.S.  1987.  Aging, Leydig

cell structure-function and benign prostatic hyperplasia in beagles.  In: Benign Prostatic Hyperplasia, Vol. II, eds. C.H. Rodger, D.S. Coffey, G. Cunha, J.T. Grayhack, F.

Hinman, Jr. and R. Horton.  Publication No. 87-2881, USDHHS, NIH, Bethesda, MD,

pp. 129-137.

10. Zirkin, B.R., Perreault, S.D. and Naish, S.J.  1988.  Formation and function of the male pronucleus during mammalian fertilization.  In: The Molecular Biology of Fertilization

(eds. G. Schatten and H. Schatten), Academic Press, NY, pp. 91-114.

11. Perreault, S.D., Naish, S.J. and Zirkin, B.R.  1988.  Fertilization by sperm microinjection and

zona drilling: Applications in the basic and clinical sciences.  In: Hands-On IVF, Cryopreservation and Micromanipulation.  University of Wisconsin Biotechnology Center, Series No. 2, eds. N.L.F. First and A.H. DeCherney, pp. 113-121.

12. Kelce, W.R., Zirkin, B.R. and Ewing, L.L. 1991.  Leydig cell primary cultures can be used to

identify toxicants acting to impaire or kill Leydig cells.  In: Goldberg, A.M., ed.  In vitro toxicology: mechanisms and new technology.  New York: Mary Ann Liebert, Inc., pp.

397-407.

13. Roberts, K.P. and Zirkin, B.R.  1991.  Androgen regulation of spermatogenesis in the rat. Ann.  N.Y. Acad. Sci. 637: 90-106.

14. Hardy, M.P., Nonneman, D., Ganjam, V.K. and Zirkin, B.R. 1993.  Hormonal control of Leydig cell differentiation and mature function. Serono Symp. 98:125-142.

15. Zirkin, B.R.  1993. Regulation of spermatogenesis in the adult mammal: gonadotropins and

androgens. In: Cell and Molecular Biology of Reproduc tion (ed. Desjardins, C.C. and Ewing, L.L.), Oxford University Press, pp. 166-188.

16. Zirkin, B.R. 1994.  Hypothalamo-Pituitary-Testicular Axis.  In: Handbook, American Society

of Andrology.

17. Chen, H., Luo, L. and Zirkin, B.R. 1996.  Leydig cell structure and function during aging. In: The Leydig Cell (Payne, A.H., Hardy, M.P. and Russell, L.D., eds.).  Cache River Press, IL.,  pp. 221-230.

18. Hardy, M.P. and Zirkin, B.R.  1997.  Leydig cell function.  In: Infertility in the Male (Lipshultz, L.I. and Howards, S.S., eds.), 3rd Edition, Mosby, pp. 59-70.

19. Zirkin, B.R., Brown, T.R., Chen, H., Luo, L., Banerjee, P.P. and Banerjee, S. 1997. Reproductive aging in the male.  In: Current Advances in Andrology: Proceedings of the

VIth International Congress of Andrology (Waites, G.M.H., Frick, J. And Baker, G.W.H., eds),  Monduzzi Editore, Italy, pp. 275-284.

20. Zirkin, B.R., Chen, H. and Luo, L. 1997.  Leydig cell steroidogenesis in aging rats.  Exp. Gerontol. 32: 529-537.

21. Chen, H., Luo, L. and Zirkin, B.R. 1998. Testicular aging: Leydig cells and spermatogenesis.  In: Germ Cell Development, Division, Disruption and Death (ed. Zirkin, B.R.), Serono Symposia USA, Springer, pp. 130-139.

22. Zirkin, B.R. 1998.  Spermatogenesis - Its Regulation by Testosterone and FSH.  In: Seminars

in Cell and Developmental Biology - The Male Germ Cell: Migration to Fertilization  (ed. Bellve, A.R.), Vol. 9, Academic Press, pp. 417-421.

23. Zirkin, B.R. 1998.  Spermatogenesis, Hormonal Control of.  In: Encyclopedia of Reproduction (eds. Knobil, E. And Neill, J.D.), Vol. 4, Academic Press, pp. 556-563.

24. Zirkin, B.R. and Chen, H. 2000.  Regulation of Leydig cell structure and function. Biol. Reprod. 63: 977-981.

25. Zirkin, B. R. and Robaire, B.  2000. Unlocking the mysteries of male reproductive function: How far have we come and where are we going?  In: The Testis: From Stem Cell to Sperm Function (Goldberg, E., ed.).  Springer-Verlag, New York, NY, pp.3-9.

26. Mitchell, A., Bakshi, K., Kimmel, kC., Buck, G., Feuston, M., Foster, P.M., Friedman, J., Hughes, C., Moore, J., Schwetz, B., Scialli, A., Scott, W., Vorhees, C. and Zirkin, B. 2004.  Evaluating chemical and other agent exposures for reproductive and developmental toxicity.  J. Toxicol.. Environ. Health A. 67: 1159-1314.

 

Journal Articles (peer review)

1. Zirkin, B.R. 1970.  The protein composition of nuclei during spermiogenesis in the leopard frog, Rana pipiens.  Chromosoma 31: 231-240.

2. Zirkin, B.R. and Wolfe, S.L. 1970.  The chemical composition of nuclei and chromosomes isolated by the Langmuir trough technique.  Chromosoma 32: 162-170.

3. Zirkin, B.R. 1971.  The fine structure of nuclei during spermiogenesis in the leopard frog, Rana pipiens.  J. Ultrastruct. Res. 34: 159-174.

4. Zirkin, B.R. 1971.  The fine structure of nuclei in mature sperm.  l. Application of the Langmuir trough-critical point method to histone-containing sperm nuclei.  J. Ultrastruct.

Res. 36: 237-248.

5. Zirkin, B.R. and Wolfe, S.L. 1972.  Fiber ultrastructure and dimensions in thin-sectioned chromatin.  J. Ultrastruct. Res. 39: 496-508.

6. Zirkin, B.R. and Kim, S.K. 1972.  Fine structure of fibers in thin sections of condensed chromatin Exp. Cell Res. 75: 490-496.

7. Zirkin, B.R. 1973.  A cytochemical study of the nonhistone protein content of condensed and

extended chromatin.  Exp. Cell Res. 78: 394-398.

8. Zirkin, B.R. 1975.  The ultrastructure of nuclear differentiation during spermiogenesis in the

salmon.  J. Ultrastruct. Res. 50: 174-184.

9. Zirkin, B.R. 1975.  The use of glycol methacrylate embedding for studies of chromatin distribution and fine structure in salmon spermatid nuclei.  Histochemistry 44: 137-146.

10. Heston, W.D.W., Zirkin, B.R., and Coffey, D.S. 1975.  Release of chromatin template

restrictions in rabbit spermatozoa.  Biochem. Biophys. Res. Comm. 64: 162-168.

11. Zirkin, B.R., Boison, A., Heston, W.D.W., and Coffey, D.S. 1976.  Release of DNA template

restrictions in rabbit spermatozoa and rat liver nuclei.  J. Exp. Zool. 197: 283-288.

12. Robaire, B., Ewing, L.L., Zirkin, B.R. and Irby, D.S. 1977.  D4-5a-reductase and 3a-

hydroxysteroid dehydrogenase in the rat epididymis.  Endocrinology 101: 1379-1390.

13. Zirkin, B.R. and Chang, T.S.K. 1977.  Involvement of endogenous proteolytic activity in

thiol-induced release of DNA template restrictions in rabbit sperm nuclei.  Biol. Reprod. 17: 131-137.

14. Chang, T.S.K. and Zirkin, B.R. 1977.  Proteolytic degradation of protamine during thiol

-induced nuclear decondensation in rabbit spermatozoa.  J. Exp. Zool. 204: 283-290.

15. Chang, T.S.K. and Zirkin, B.R. 1978.  Distribution of sulfhydryl oxidase activity in the rat and hamster male reproductive tract.  Biol. Reprod. 17: 745-748.

16. Mayer, J.F., Jr. and Zirkin, B.R.  1979.  Spermatogenesis in the mouse. l.  Nuclear incorporation and loss of ;H-amino acids.  J. Cell Biol. 81: 403-410.

17. DeKlerk, D.P., Coffey, D.S., Ewing, L.L., McDermott, I., Reiner, W.G., Robinson, C.H.,

Scott, W.W., Strandberg, J.D., Talalay, P., Walsh, P.C., Wheaton, L.G., and Zirkin, B.R.       1979. A comparison of spontaneous and experimentally induced canine prostatic hyperplasia.  J. Clin. Inv. 64: 842-849.

18. Ewing, L.L., Zirkin, B.R., Cochran, R.C., Kromann, N., Peters, C. and Ruiz-Bravo, N. 1979.

Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro: Correlation with Leydig cell mass.  Endocrinology 105: 1135-1142.

19. Zirkin, B.R., Chang, T.S.K. and Heaps, J. 1980.  Involvement of an acrosin-like proteinase in

the sulfhydryl-induced degradation of rabbit sperm nuclear protamine.  J. Cell Biol. 85: 116-121.

20. Zirkin, B.R., Ewing, L.L., Kromann, N. and Cochran R.  1980.  Testosterone secretion by rat,

rabbit, guinea pig, dog and hamster testes perfused in vitro: Correlation with Leydig cell ultrastructure.  Endocrinology 107: 1867-1874.

21. Robaire, B. and Zirkin, B.R. 1981.  Hypophysectomy and simultaneous testosterone replacement: Effects on male rat reproductive tract and epididymal D4-5a reductase and

3a-hydroxysteroid dehydrogenase.  Endocrinology 109: 1225-1233.

22. Ewing, L.L., Zirkin, B.R. and Chubb, C. 1981. Assessment of testicular testosterone

production and Leydig cell structure. Env. Hlth. Persp. 38: 19-27.

23. Mayer, J.F., Chang, T.S.K. and Zirkin, B.R. 1981.  Spermatogenesis in the mouse. 2. Amino

acid incorporation into basic nucleoproteins of mouse spermatids and spermatozoa. Biol.

Reprod. 25: 1041-1051.

24. Marshall, F.F., Ewing, L.L., Zirkin, B.R. and Cochran, R.C. 1982.  Testicular atrophy associated with agenesis of the epididymis in the ACI rat.  J. Urol. 127: 155-158.

25. Perreault, S.D., Zirkin, B.R. and Rogers, B.J. 1982.  Effect of trypsin inhibitors on acrosome

reaction of guinea pig spermatozoa.  Biol. Reprod. 26: 343-351.

26. Perreault, S.D. and Zirkin, B.R. 1982.  Sperm nuclear decondensation in mammals: Role of

sperm-associated proteinase in vivo.  J. Exp. Zool. 224:253-257.

27. Ewing, L.L., Wing, T.-Y., Cochran, R.C., Kromann, N. and Zirkin,  B.R.  1983.  Effect of

luteinizing hormone on Leydig cell structure and testosterone secretion.  Endocrinology 112:1763-1769.

28. Brendler, C.B., Berry, S.J., Ewing, L.L., McCullough, A.R., Cochran, R.C., Strandberg, J.D.,

Zirkin, B.R., Coffey, D.S., Wheaton, L.G., Hiler, M.L., Bordy, M.J., Scott, W.W. and Walsh, P.C. 1983.  Spontaneous benign prostatic hyperplasia in the beagle: age- associated changes in serum hormone levels, and the morphology and secretory function of the  canine prostate.  J. Clin. Invest. 71:1114-1123.

29. Holt, J.A., Schreiber, J.R. and Zirkin, B.R. 1983.  Estradiol-induced changes in rabbit luteal

cell progestin production and cholesterol and cholesterol ester content.  Biochem. Biophys. Res. Comm. 113:1026-1033.

30. Perreault, S.D. and Zirkin, B.R.  1984. The role of disulfide bond reduction during sperm

nuclear decondensation in vivo.  Dev. Biol. 101:160-167.

31. Ewing, L.L., Thompson, D.L., Cochran, R.C., Lasley, B.L. and Zirkin, B.R. 1984.  Testicular

steroid production and benign prostatic hyperplasia in the beagle.  Endocrinology 114:1308-1314.

32. Zirkin, B.R. and Strandberg, J.D. 1984. Quantitative changes in the morphology of the aging canine prostate.  Anat. Rec. 208:207-214.

33. Bronstein, R.D., Holt, J.A. and Zirkin, B.R.  1984.  Estradiol-induced changes in progesterone secretion by rabbit corpora lutea are associated with quantitative ultrastructural changes in luteal cells.  Endocrinology 115:342-349.

34. Wing, T.-Y., Ewing, L.L. and Zirkin, B.R.  1984.  Effects of LH withdrawal on Leydig cell

smooth endoplasmic reticulum and steroidogenic reactions which convert pregnenolone to testosterone.  Endocrinology 115:2290-2296.

35. Zirkin, B.R., Gross, R. and Ewing, L.L.  1985.  Effects of lead acetate on male reproduction.

Concepts Toxicol. 3:138-145.

36. Zirkin, B.R., Soucek, D.A., Chang, T.S.K. and Perreault, S.D.  1985.  In vitro and in vivo

studies of mammalian sperm nuclear decondensation.  Gamete Res. 11:349-365.

37. Wing, T.-Y., Ewing, L.L., Zegeye, B. and Zirkin, B.R.  1985. Restoration effect of exogenous

LH on testicular steroidogenesis and Leydig cell ultrastructure.  Endocrinology 117:1779-1787.

38. Jarow, J.P., Budin, R., Dym, M., Zirkin, B.R., Noren, S. and Marshall, F.F.  1985.

Quantitative pathologic changes in the human testis following vasectomy.  A controlled study.  New England J. Med. 313:1252-1256.

39. Adashi, E.Y., Resnick, C.E. and Zirkin, B.R.  1987.  Antigonadal activity of the neurohypophysial hormones: In vivo regulation of testicular function of hypophysectomized rats. Biol. Reprod. 37:935-946.

40. Perreault, S.D., Naish, S.J. and Zirkin, B.R.  1987.  The timing of hamster sperm nuclear

decondensation and male pronuclear formation are related to sperm nuclear disulfide bond content.  Biol. Reprod. 36:239-244.

41. Naish, S.J., Perreault, S.D., Foehner, A. and Zirkin, B.R.  1987.  DNA synthesis by the

fertilizing sperm nucleus: Relationship to sperm nuclear disulfide bond content.  Biol. Reprod.  36:245-253.

42. Zirkin, B.R. and Ewing, L.L.  1987.  Leydig cell differentiation during maturation of the rat

testis: A stereological study of cell number and ultrastructure.  Anat. Rec. 219:157-163.

43. Naish, S.J., Perreault, S.D. and Zirkin, B.R.  1987.  DNA synthesis following microinjection

of heterologous sperm and somatic cell nuclei into hamster oocytes.  Gamete Res. 18:109-120.

44. Mendis-Handagama, S.M.L.C., Zirkin, B.R. and Ewing, L.L.  1988. Comparison of components of the testis interstitium with testosterone secretion in hamster, rat and guinea pig testes perfused in vitro.  Am. J. Anat. 181:12-22.

45. Hardy, M.P., Mendis-Handagama, S.M.L.C., Zirkin, B.R. and Ewing, L.L.  1988. Photoperiodic variation in Leydig cell numbers in the testis of the golden hamster: A possible

mechanism for their renewal during recrudescence.  J. Exp. Zool. 244:269-276.

46. Keeney, D.S., Mendis-Handagama, S.M.L.C., Zirkin, B.R. and Ewing, L.L.  1988.  Effect of

long-term deprivation of luteinizing hormone on Leydig cell volume, Leydig cell number and steroid­ogenic capacity of the rat testis.  Endocrinology 123:2906-2915.

47. Dharmarajan, A.M., Mastroyannis, C., Yoshimura, Y., Atlas, S.J., Wallach, E.E. and Zirkin, B.R.  1988. Quantitative light micro­scopic analysis of corpus luteum growth during pseudopregnancy in the rabbit.  Biol. Reprod. 38:863-870.

48. Dharmarajan, A.M., Yoshimura, Y., Sueoka, K., Atlas, S.J., Dubin, N.H., Ewing, L.L., Zirkin, B.R. and Wallach, E.E.  1988.  Progesterone secretion by corpora lutea of the isolated

perfused rabbit ovary during pseudopregnancy.  Biol. Reprod. 38:1137-1143.

49. Walters, J.R., Juniewicz, P.E., Oesterling, J.E., Mendis-Handagama, S.M.L.C., Zirkin, B.R. and Ewing, L.L.  1988.  The effect of inhibition of aromatase enzyme activity on Leydig cell

number and ultrastructure in beagles.  Endocrinology 123:2223-2229.

50. Awoniyi, C.A., Santulli, R., Sprando, R.L., Ewing, L.L. and Zirkin, B.R.  1989.  Restoration of advanced spermatogenic cells in the experimentally regressed rat testis: Quantitative relationship to testosterone concentration within the testis.  Endocrinology 124:1217-1223.

51. Hardy, M.P., Zirkin, B.R. and Ewing, L.L.  1989.  Kinetic studies on the development of the

adult population of Leydig cells in testes of the pubertal rat.  Endocrinology 124:762-770.

52. Dharmarajan, A.M., Sueoka, K., Miyazaki, T., Atlas, S.J., Ghodgaonkar, R.B., Dubin, N.H.,

Zirkin, B.R. and Wallach, E.E.  1989.  Prostaglandins and progesterone secretion in the in vitro perfused pseudopregnant rabbit ovary.  Endocrinology 124:1198-1203.

53. Zirkin, B.R., Santulli, R., Awoniyi, C.A. and Ewing, L.L.  1989. Maintenance of advanced

spermatogenic cells in the adult rat testis: Quantitative relationship to testosterone concentration within the testis.  Endocrinology 124:3043-3049.

54. Awoniyi, C.A., Santulli, R., Chandrashekar, V., Schanbacher, B.D. and Zirkin, B.R.  1989.

Quantitative restoration of advanced spermatogenic cells in adult male rats made azoospermic by active immunization against luteinizing hormone or gonadotropin- releasing hormone. Endocrinology 125:1303-1309.

55. Santulli, R., Sprando, R.L., Awoniyi, C.A., Ewing, L.L. and Zirkin, B.R. 1989.  To what extent can spermatogenesis be maintained in the hypophysecto- mized adult rat testis with exogenously administered testosterone?  Endocrinology 126:95-101.

56. Keeney, D.S., Sprando, R.L., Robaire, B., Zirkin, B.R. and Ewing, L.L. 1990.  Reversal of long- term luteinizing hormone deprivation on testosterone secretion and Leydig cell volume,

number and proliferation in adult rats.  J. Endocrinology 127:47-58.

57. Sprando, R.L., Santulli, R., Awoniyi, C.A., Ewing, L.L. and Zirkin, B.R.  1990.  Does ethane

1,2-dimethanesulphonate (EDS) have a direct cytotoxic effect on the seminiferous epithelium of the rat testis?  J. Androl. 11:344-352.

58. Mendis-Handagama, S.M.L.C., Zirkin, B.R., Scallen, T.J. and Ewing, L.L.  1990.  Studies on

peroxisomes of the adult rat Leydig cell.  J. Androl. 11:270-278.

59. Mendis-Handagama, S.M.L.C., Watkins, P.A., Gelber, S.J., Scallen, T.J., Zirkin, B.R. and

Ewing, L.L.  1990.  Luteinizing hormone causes rapid and transient changes in rat Leydig cell peroxisome volume and intraperoxisomal sterol carrier protein-2 content. Endocrinology 127:2947-2954.

60. Awoniyi, C.A., Sprando, R.L., Santulli, R. and Zirkin, B.R. 1990. Restoration of spermatogenesis by exogenously administered testosterone in rats made azoospermic by

hypophysectomy or by withdrawal of luteinizing hormone alone.  Endocrinology 127:177-184.

61. Banerjee, P.P., Banerjee, S., Sprando, R.L. and Zirkin, B.R.  1991. Regional cellular heterogeneity and DNA synthetic activity in rat ventral prostate during postnatal development.  Biol. Reprod. 45:773-782.

62. Roberts, K.P., Awoniyi, C.A., Santulli, R. and Zirkin, B.R.  1991. Regulation of Sertoli cell

transferrin and sulfated glycoprotein-2 messenger RNA levels during the restoration of spermatogenesis in the adult hypophysectomized rat.  Endocrinology 129:3417-3423.

63. Dharmarajan, A.M., Zanagnolo, V.L., Dasko, L.M., Zirkin, B.R., Ewing, L.L. and Wallach, E.E. 1991.  Estradiol regulation of the rabbit corpus luteum: in vivo and in vitro studies. Endocrinology 128:2678-2684.

64. Kelce, W.R., Zirkin, B.R. and Ewing, L.L. 1991.  Immature rat Leydig cells are intrinsically less sensitive than adult Leydig cells to ethane dimethanesul-fonate.  Tox. Appl. Pharmacol. 111: 189-200.

65. Kelce, W.R., Zirkin, B.R. and Ewing, L.L.  1991. Leydig cell primary cultures can be used to

identify toxicants acting to impair or kill Leydig cells.  Alternative Methods in Toxicology 8:397-407.

66. Roberts, K.P., Santulli, R., Seiden, J. and Zirkin, B.R. 1992.  The effect of testosterone

withdrawal and subsequent germ cell depletion on transferrin and sulfated glycoprotein-2 messenger ribonucleic acid levels in the adult rat testis.  Biol. Reprod. 47:92-96.

67. Trasler, J.M., Alcivar, A.A., Awoniyi, C.A., Santulli, R., Zirkin, B.R. and Hecht, N.B. 1992.

Temporal gene expression is restored concomitantly with germ cells in the experimentally regressed rat testis.  Endocrinology 131:  297-304.

68. Amann, R.P., Deaver, D.R., Zirkin, B.R., Grills, G.S., Sapp, W.J., Veeramachaneni, D.N.R.,

Clemens, J.W., Banerjee, S.D., Folmer, J., Gruppi, C.M., Wolgemuth, D.J., Williams, C.S. and Serova, L.V.  1992. Effects of microgravity or simulated launch on testicular function in rats.  J. Appl. Physiol. 73:1745-1855.

69. Awoniyi, C.A., Zirkin, B.R., Chandrashekar, V. and Schlaff, W.D. 1992. Exogenously

administered testosterone maintains spermatogenesis quantitatively in adult rats actively immunized against gonadotropin-releasing hormone.  Endocrinology 130: 3283-3288.

70. Sharma, O.P., Adlercreutz, H., Strandberg, J.D., Zirkin, B.R., Coffey, D.S. and Ewing, L.L.

1992. Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats.  J. Steroid Biochem. Molec. Biol. 43:557-564.

71. Banerjee, P.P., Banerjee, S. and Zirkin, B.R.  1993. DNA synthesis occurs throughout the rat

ventral prostate during its postnatal development. Biol. Reprod. 48:248-251.

72. Kelce, W.R. and  Zirkin, B.R. 1993.  Mechanism by which ethane dimethane- sulfonate kills

adult rat Leydig cells: Involvement of intracellular glutathione.  Tox. Appl. Pharmacol. 120:80-88.

73. Zirkin, B.R., Santulli, R., Strandberg, J.D., Wright, W.W. and Ewing, L.L.  1993.  Testicular

steroidogenesis in the aging Brown Norway rat.  J. Androl. 14:118-123.

74. Wright, W.W., Fiore, C. and Zirkin, B.R.  1993. The effect of aging on the seminiferous epithelium of the Brown Norway rat.  J. Androl. 14:110-117.

75. Roberts, K.P. and Zirkin, B.R.  1993. Androgen binding protein inhibits androgen-dependent

transcription in MSC-1 cells: Implications for ABP action in vivo.  Endocrine J. 1:41-47.

76. Banerjee, S., Banerjee, P.P. and Zirkin, B.R.  1993. Cell proliferation in the dorsal and lateral

lobes of the rat prostate during postnatal development.  J. Androl. 14:310-318.

77. Chen, H., Chandrashekar, V. and Zirkin, B.R.  1994.  Can spermatogenesis be maintained quantitatively in intact adult rats with exogenously administered dihydrotestosterone?  J. Androl. 15:132-138.

78. Banerjee, P.P., Banerjee, S., Dorsey, R., Zirkin, B.R. and Brown, T.R.  1994. Age- and lobe-

specific responses of the Brown Norway rat prostate to androgen.  Biol. Reprod. 51:675-684.

79. Zirkin, B.R., Awoniyi, C., Griswold, M.D., Russell, L.D. and Sharpe, R.  1994.  Is FSH required for adult spermatogenesis?  J. Androl. 15:273-276.

80. Jarow, J.P., Keeney, D.S., Robaire, B., Ewing, L.L. and Zirkin, B.R.  1994. In vivo model for chronic direct intratesticular drug administration.  J. Androl. 15:456-461.

81. Chen, H., Hardy, M.P., Huhtaniemi, I. and Zirkin, B.R.  1994. Age-related decreased Leydig cell testosterone production in the Brown Norway rat.  J. Androl. 15:551-557.

82. Banerjee, P.P., Banerjee, S., Tilly, K.I., Tilly, J.L., Brown, T.R. and Zirkin, B.R.  1995. Lobe-

specific apoptotic cell death in rat prostate after androgen ablation by castration. Endocrinology 136:4368-4376.

83. Roberts, K.P., Banerjee, P.P., Tindall, J.W.M. and Zirkin, B.R.  1995.  Immortalization and characterization of a Sertoli cell line from the adult rat.  Biol. Reprod. 53:1446-1453.

84. Darney, K.J., Zirkin, B.R. and Ewing, L.L.  1996.  Testosterone autoregulation of its biosynthesis in the rat testis: inhibition of 17a-hydroxylase activity.  J. Androl. 17:137-142.

85. Chen, H.L, Huhtaniemi, I. and Zirkin, B.R.  1996.  Depletion and repopulation of Leydig cells in the testes of aging Brown Norway rats.  Endocrinology 137:3447-3452.

86. Alcivar-Warren, A.A., Trasler, J.M., Awoniyi, C.A., Zirkin, B.R. and Hecht, N.B.  1996. Differential expression of ornithine decarboxylase, poly (ADP) ribose polymerase, and mitochondrial mRNAs following testosterone administration to hypophysectomized rats. Molec. Reprod. Dvpt. 43:283-289.

87. Luo, L., Chen, H.L. and Zirkin, B.R.  1996.  Are Leydig cell steroidogenic enzymes

Differentially regulated with aging?  J. Androl. 17:509-515.

88. Sprando, R.L. and Zirkin, B.R.  1997. Is Leydig cell steroidogenic function affected by the germ cell content of the seminiferous tubules?  J. Androl. 18: 424-430.

89. Zirkin, B.R., Chen, H.L. and Luo, L.  1997. Leydig cell steroidogenesis in aging rats. Exp. Gerontol. 32: 529-537.

90. Banerjee, S., Banerjee, P.P., Zirkin, B.R. and Brown, T.R.  1998. Regional expression of transforming growth factor-a in rat ventral prostate during postnatal development, after androgen ablation, and after androgen replacement.  Endocrinology 139: 3005-3103.

91. Banerjee, P.P., Banerjee, S., Zirkin, B.R. and Brown, T.R. 1998. Telomerase activity in normal adult Brown Norway rat seminal vesicle: regional distribution and age-dependent changes. Endocrinology 139: 1075-1081.

92. Banerjee, P.P., Banerjee, S., Lai, J.M., Strandberg, J.D., Zirkin, B.R. and Brown, T.R. 1998.

Age-dependent and lobe-specific spontaneous hyperplasia in the Brown Norway rat prostate.  Biol. Reprod. 59: 1163‑1170.

93. Banerjee, P.P., Banerjee, S.,  Zirkin, B.R. and Brown, T.R.  1998. Lobe-specific telomerase

activity in the intact adult Brown Norway rat prostate and its regional distribution within the prostatic ducts. Endocrinology 139: 513-519.

94. Luo, L., Chen, H., Stocco, D. and Zirkin, B.R.  1998. Leydig cell protein synthesis and steroidogenesis in response to acute stimulation by luteinizing hormone in rats.  Biol. Reprod. 59: 263-270.

95. Grzywacz, F.W., Chen, H., Allegretti, J. and Zirkin, B.R. 1998. Does age-associated reduced

Leydig cell testosterone production in Brown Norway rats result from under-stimulation by luteinizing hormone?  J. Androl. 19: 625‑630.

96. Friedmann, A.S., Chen, H.L.,  Rabuck,  L.D.  and Zirkin,  B.R.  1998.  Accumulation of

dietary methylmercury in the testes of the adult Brown Norway rat- impaired testicular and epididymal function. Environ. Tox.Chem. 17: 867‑871.

97. Young, K.A., Zirkin, B.R. and Nelson, R.J.  1999. Short photoperiods evoke testicular apoptosis in white-footed mice (Peromyscus leucopus).  Endocrinology 140: 3133-3139.

98. Nandi, S., Banerjee, P.P. and Zirkin, B.R. 1999.  Germ cell apoptosis in the testes of Sprague Dawley rats following testosterone withdrawal by ethane 1,2-dimethanesulfonate administration: relationship to Fas?  Biol. Reprod. 61: 70-75.

99. Chen, H. and Zirkin, B.R. 1999.  Long-term suppression of Leydig cell steroidogenesis

prevents Leydig cell aging.  Proc. Nat. Acad. Sci. 96: 14877-14881.

100. Young, K.A., Zirkin, B.R. and Nelson, R.J. 2000. Testicular regression in response to food

restriction and short photoperiod in white-footed mice (Peromyscus leucopus) is mediated by apoptosis.  Biol. Reprod. 62: 347-354.

101. Kim, J.-M., Luo, L. and Zirkin, B.R. 2000. Caspase-3 activation and its nuclear translocation are required for Leydig cell apoptosis induced by ethane dimethanesulfonate.  Endocrinology 141: 1846‑1853.

102. Jarow, J.P., Chen, H., Rosner, W., Trentacoste, S. and Zirkin, B.R.  2001. Assessment of the

androgen environment within the human testis: minimally invasive method to obtain intratesticular fluid. J Androl. 22: 640-645.

103. Trentacoste, S., Friedmann, A.S., Youker, R.T., Breckenridge, C.B., and Zirkin, B.R. 2001. Atrazine effects on testosterone and androgen-dependent reproductive organs in peripubertal male rats. J. Androl. 22: 142-148.

104. Luo, L., Chen, H. and Zirkin, B.R. 2001. Leydig cell aging: Steroidogenic acute regulatory

protein (StAR) and cholesterol side-chain cleavage enzyme. 2001. J. Androl. 22: 149-156.

105. Chen, H., Cangello, D., Benson, S., Folmer, J., Zhu, H., Trush, M.A. and Zirkin, B.R.  2001.

Age-related increase in mitochondrial superoxide generation in the testosterone-producing cells of Brown Norway rat testes: relationship to reduced steroidogenic function? Exper. Gerontol. 36: 1361-1373.

106. Young, K.A., Zirkin, B.R. and Nelson, R.J.  2001. Testicular apoptosis is downregulated during             spontaneous recrudescence in white-footed mice (Peromyscus leucopus).  J. Biol. Rhythms 16: 479-488.

107. Akingbemi, B.T., Youker, R., Scottas, C., Ge, R., Katz, E., Klinefelter, G.R., Zirkin, B.R. and Hardy, M.P. 2001. Modulation of rat Leydig cell steroidogenic function by di(2-ethylhexyl)phthalate. Biol Reprod. 65:1252-1259.

108. Syntin, P., Chen, H., Zirkin, B.R. and Robaire, B.  2001. Gene expression in Brown Norway rat Leydig cells: Effects of age and age-related germ cell loss.  Endocrinology 142: 5277-5285.

109. Kim, J-M., Ghosh, S.R., Weil, A.C.P. and Zirkin, B.R. 2001. Caspase-3 and caspase-activated deoxyribonuclease are associated with testicular germ cell apoptosis resulting from reduced intratesticular testosterone.  Endocrinology 142: 3809-3816.

110. Culty, M., Luo, L., Yao, Z.X., Chen, H., Papadopoulos, V. and Zirkin, B.R. 2002. Cholesterol transport, peripheral benzodiazepine receptor, and steroidogenesis in aging Leydig  cells. J. Androl. 23: 439-447.

111. Chen H, Hardy MP, Zirkin BR. 2002. Age-related decreases in Leydig cell testosterone production are not restored by exposure to LH in vitro. Endocrinology 143:1637-1642.

112. Anway, M.D., Folmer, J. and Zirkin, B.R. 2003. Isolation of Sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function. Biol. Reprod.68: 996-1002.

113. Show, M.D., Anway, M.D., Folmer, J.S. and Zirkin, B.R.  2003. Reduced intratesticular testosterone concentration alters the polymerization state of the Sertoli cell intermediate filament cytoskeleton by degradation of vimentin.  Endocrinology 144: 5530-5536.

114. Anway, M.D., Wright, W.W., Zirkin, B.R., Korah, N., Mort, J.S. and Hermo, L. 2004. Expression and localization of cathepsin K in adult rat Sertoli cell.  Biol. Reprod. 70: 562-569.

115. Chen, H., Irizarry, R.A., Luo, L. and Zirkin, B.R. 2004. Leydig cell gene expression: effects of age and caloric restriction. caloric restriction.  Exp. Gerontol. 39: 31-43.

116. Akingbemi, B.T., Klinefelter, G.R., Zirkin, B.R. and Hardy, M.P. 2004. Phthalate-induced Leydig cell hyperplasia is associated with multiple endocrine disturbances. Proc Natl. Acad. Sci. 101:775-780.

117. Show, M.D., Folmer, J., Anway, M.D. and Zirkin, B.R.  2004. Testicular expression and distribution of the rat Bcl2 Modifying Factor (BMF) in response to reduced intratesticular testosterone.  Biol. Reprod. 70: 1153-1161.

118. Somwaru, L. Li, S., Doglio, L., Goldberg, E. and Zirkin, B.R. 2004. Heat-induced apoptosis of mouse meiotic cells is suppressed by ectopic expression of testis-specific calpastatin. J. Andrology 25: 506-513. 

119. Chen, H., Liu, J., Luo, L. and Zirkin, B.R. 2004. Dibutyryl cyclic adenosine monophosphate restores the ability of aged Leydig cells to produce testosterone at the high levels characteristic of young cells.  Endocrinology 145: 4441-4446.

120. Hill, C.M., Anway, M.D., Zirkin, B.R. and Brown, T.R.  2004. Intratesticular androgen levels, androgen receptor localization, and androgen receptor expression in adult rat Sertoli cells.  Biol. Reprod. 71: 1348-1358.

121.  Coviello, A.D., Bremner, W.J., Matsumoto, A.M., Herbst, K.L., Amory, J.K., Anawalt, B.D., Yan, X., Brown, T.R., Wright, W.W., Zirkin, B.R. and Jarow, J.P. 2004.  Intratesticular testosterone concentrations comparable with serum levels are not sufficient to maintain normal sperm production in men receiving a hormonal contraceptive regimen.  J. Andrology 25: 931-938.

121. Zhao, M. Baker, S.D., Yan, X. Zhao, Y. Wright, W.W., Zirkin, B.R. and Jarow, J.P.  2004. Simultaneous determination of steroid composition of human testicular fluid using liquid chromatography tandem mass spectrometry.  Steroids 69: 721-726.

122.  Show, M.D., Anway, M.D. and Zirkin, B.R. 2004.  An ex vivo analysis of Sertoli cell actin dynamics following gonadotropic hormone withdrawal.  J. Andrology 25: 1013-1021.

123.  Luo, L., Chen, H. and Zirkin, B.R. 2005. Temporal relationships among testosterone production, steroidogenic acute regulatory protein (StAR), and P450 side-chain cleavage enzyme (P450scc) during Leydig cell aging.  J. Andrology 26: 25-31.

124. Liu, Y., Yao, Z-X., Bendavid, C., Borgmeyer, C., Han, Z., Cavalli, L.R., Chan, W-Y., Folmer, J., Zirkin, B.R., Haddad, B.R., Gallicano, G.I., Folmer, J., Zirkin, B.R. and Papadopoulos, V.  Cytochrome P450 17a-hydroxylase/17,20 lyase (CYP17) gene deletion causes infertility in mice.  Mol. Endocrinol., submitted.

125.  Coviello, A., Wright, W.W., Brown, T., Yan, X., Amory, J.K., Anawalt, B.D., Bremner, W., Matsumoto, A., Zirkin, B.R. and Jarow, J.P.  Maintenance of intratesticular testosterone concentration by gonadotropin administration in man.  J. Clin. Endocrinol. Metab., in press.

126. Jarow., J.P., Wright, W.W., Brown, T.R., Yan, X. and Zirkin, B.R. Bioactivity of androgens within the testes and serum of normal men.  J. Andrology, in press.

127.  Coviello, A.D. Amory, J.K.,  Herbst, K.L., Anawalt, B.D., Matsumoto, A.M., Bremner, W.J., Brown, T.R., Wright, W.W., Zirkin, B.R.  and Jarow, J.P. Intratesticular testosterone and bioactive androgen concentrations in response to contraceptive doses of testosterone enanthate and levonorgestrel in men:  testosterone concentrations comparable to serum levels are not sufficient to maintain normal sperm production.  J. Andrology, submitted.

128.  Chen, H. and Zirkin, B.R. Leydig cell steroidogenesis: effects of age and caloric restriction. Exp. Gerontol., submitted.

129.  Coviello, A.D., Matsumoto, A.M., Bremner, W.J., Herbst, K.L., Amory, J.K., Anawalt, B.D., Sutton, P.L., Wright, W.W., Brown, T.R., Yan, X., Zirkin, B.R. and Jarow, J.P.  Low dose human chorionic gonadotropin maintains intratesticular testosterone in normal men with gonadotropin suppression.  J. Andrology, submitted.

130.  Anway, M.D., Show, M. and Zirkin, B.R.  Protein C inhibitor expression by adult rat Sertoli cells: effects of testosterone withdrawal and replacement. J. Andrology, submitted.

131.  Chen, H., Baig, M.U., Liu, J., Luo, L. and Zirkin, B.R. Aging, vitamin E and Leydig cell steroidogenesis.  Exp. Gerontol., submitted.

132.  Luo, L. Show, M.D., Anway, M.D., Chen, H., Trush, M. and Zirkin, B.R. Aging and the Leydig cell antioxidant system.  J. Andrology, submitted. 

 

Other

1.  Zirkin, B.R. and Robaire, B.  1991. A Thirst for Science: An overview of the scientific contributions of Larry L. Ewing (1936-1990). Ann. N.Y. Acad. Sci. 637: 1-16.

2.  Zirkin, B.R.  1992. Remembrance: Scientific Contributions of Larry L. Ewing (1936-1990). Endocrinology 130:2435-2437.

3.  Zirkin, B.R. 1994.  Book Review: Methods in Toxicology, Vol. 3, Part A: Male  Reproductive Toxicology.  Edited by R.E. Chapin, J.J. Heindel.

4.  Zirkin, B.R. 1998.  Choosing Baby’s Sex.  Johns Hopkins Health Insider, InteliHealth, Johns

Hopkins University and Health System, Office of Consumer Health Information.

 

 

 

 

                                                                Curriculum Vitae

 

                                                                BARRY R. ZIRKIN

 

                                                                Part II: Jan. 2004

 

 

II.A       TEACHING

 

PhD Advisees

Andrew Midzak, PhD, current

Matthew Show, PhD, current

Kelly Young (joint, with Dr. Randy Nelson), PhD, 2000

Shunping Wang (joint, with Dr. John Gearhart), PhD, 2000

Jacob Mayer, PhD, 1981

 

Master of Science (ScM) Advisees ( laboratory-based Master's program)

Brian Rosenberg, ScM, 2004.

Lily Somwaru, ScM, 2002

Shampa Ghosh, ScM, 2000

Scott Benson, ScM, 1998

Daniel Redziniak, ScM, 1998

Alex Weil, ScM, 1998

David Cangello, ScM, 1998

Frank Grzywacz, ScM, 1996

James Lai, ScM, 1996

Jan Seiden, ScM, 1992

Arnoldo Vera, ScM, 1989

Amy Foehner, ScM, 1986

Robert Bronstein, ScM, l983

 

Postdoctoral  Advisees

Dr. Matthew Anway, 2000-2003.  Currently Assistant Professor, Washington State University.

Dr. Jong-Min Kim, 1998-2002.  Currently Assistant Professor, Korea

Dr. David Wright, 2000-2001. Currently Research Scientist, Wyeth-Ayerst.

Dr. Haolin Chen, 1990-1995.  Currently Associate Scientist, Division of Reproductive Biology,

   Johns Hopkins University, School of Public Health

Dr. Lindi Luo, 1993-1995.  Currently Research Associate, Division of Reproductive Biology,

   Johns Hopkins University, School of Public Health

Dr. Andrew Friedmann, 1995-1998.  Currently Staff Scientist, Ogden Environmental and Energy

   Services, MA.

Dr. Partha Banerjee, 1989-1996.  Currently Assistant Professor, Georgetown University.

Dr. Subhadra Banerjee, 1989-1996.  Currently Staff Scientist, NIH

Dr. Kenneth Roberts, 1989-1993.  Currently Associate Professor, Department of Cell Biology

   and Neurobiology, University of Minnesota.

Dr. William Kelce, 1990-1992.  Dr. Kelce left to take the position of Reproductive Toxicologist,

   Environmental Protection Agency; and currently is Scientist,  Pfizer

Dr. Robert Sprando, Postdoctoral Fellow, 1988-1990.  Currently Research Biologist, Food and

   Drug Administration

Dr. Caleb Awoniyi, Postdoctoral Fellow, 1987-1990.  Currently Associate Professor, Department

   of Obstetrics and Gynecology, University of Colorado.

Dr. A.M. Dharmarajan, Postdoctoral Fellow, 1986-1988.  Currently Professor, Department of

   Anatomy and Human Biology, The University of Western Australia.

Dr. Sally Naish, Postdoctoral Fellow, 1984-1987.  Currently Assistant  Director-Clinical Scientist, Reproductive Endocrinology Section at Wyeth-Ayerst Research.

Dr. Sally Perreault, Postdoctoral Fellow, 1981-1984.  Currently Head, Reproductive Toxicology

   Division, Environmental Protection Agency.

Dr. Donald Soucek, Postdoctoral Fellow, 1980-1982.  Dr. Soucek is presently Professor of

   Chemistry, Truman College, Chicago.

Dr. Thomas S.K. Chang, Postdoctoral Fellow, 1976-1978.  Dr. Chang died in 1996 while

   Associate Professor of Urology, Johns Hopkins University School of Medicine.

 

Departmental Oral (2004- )

Stephanie Larson, 2004

 

Preliminary Oral (1995-)

Wen-Feng Chiu, PhD, Environmental Health Sciences, SHPH, 1995

Jackie Lavigne, PhD, Environmental Health Sciences, SHPH, 1995

Vadim Alatortsev, PhD, Div. Repro. Biol., SHPH, 1997

William Hanna, PhD, Div. Repro. Biol., SHPH, 1998

Yumbo Li, PhD, Environmental Health Sciences, SHPH, 1998

Nora Lee, PhD, Epidemiology, SHPH, 1998    

Shunping Wang, PhD, Div. Repro. Biol., SHPH, 1998

Julie Goodman, PhD, Environmental Health Sciences, SHPH, 1998

Julie Rider, PhD, Environmental Health Sciences, SHPH, 1999

Liying Zhang, PhD, Biochemistry and Molecular Biology, SHPH, 1999

Lisa Bartee, PhD, Biochemistry and Molecular Biology, 2000

Angela Howard, PhD, Environmental Health Sciences, SHPH, 2000

Martin Romeo, PhD, Biochemistry/Molecular Biology, SHPH, 2000

Xiaoling Zhu, PhD, Biochemistry/Molecular Biology, SPH, 2001

Angela Howard, PhD, Environmental Health Sciences, SPH, 2001

Christina Borgeest, U. Maryland, 2001

Courtney Johnson, PhD, Population/Family Health Sciences, SPH, 2002

Matthew Show, PhD, Biochemistry/Molecular Biology, SPH, 2002

Genevieve Wortzman, PhD, Biochemistry/Molecular Biology, SPH, 2002

Anne Sullivan, PhD, Environmental Health Sciences, SPH, 2002.

Raphael Thullier, Georgetown University, Department of Biochemistry and Molecular Biology, 2003.

 

Final Oral (1995-)

Deborah Ricker, PhD, Div. Repro. Biol., SHPH, Final Oral, 1995

Urvashi Rangan, PhD, Environmental Health Sciences, SHPH, Final Oral, 1995

Zhifeng Zhou, PhD, Div. Reproductive Biology, SHPH,  Final Oral, 1995

Laurence Zeitlin, PhD, Div. Repro. Biol., SHPH, Final Oral, 1996

Francis Grzywacz, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1996

Daniel Johnston, PhD, Div. Repro. Biol., SHPH, Final Oral, 1996

James Lai, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1996

Robert Clarke, PhD, Environmental Health Sciences, SHPH, Final Oral, 1996

Michael Seibert, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1997

Jessica Gotay, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1997

Stacy Bennett, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1998

Daniel Redziniak, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1998

Alexander Weil, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1998                                                 

Victor Nava, PhD, Molecular Microbiology and Immunology, SHPH, Thesis Reader, 1998

Henry Boateng, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1998

David Cangello, ScM, Div. Repro. Biol., SHPH, Thesis Reader, 1998

Scott Benson, ScM, Div. Repro. Biol., SHPH, Thesis Reader 1998

Sabra Klein, PhD, Psychology, A&S, Final Oral, 1998.

Lance Kriegsfeld, PhD, Dept. Psychology, A&S, Final Oral, 1999

Yunbo Li, PhD, Environmental Health Sciences, SPH, Final Oral, 1999

Kristen Jordan, PhD, Environmental Health Sciences, SPH, Final Oral, 1999

Jackie Lavigne, PhD, Environmental Health Sciences, SPH, Thesis Reader, 1999

Andrea Kalfoglou, PhD, Health Policy & Management, SPH, Final Oral, 1999

Priti Patel, ScM, Biochemistry/Molecular Biology, SPH, Thesis Reader, 1999

Shunping Wang, PhD, Biochemistry/Molecular Biology, SPH, Final Oral, 2000

Shampa Ghosh, ScM, Biochemistry/Molecular Biology, SPH, Thesis Reader, 2000

Dan Krovich, PhD,  Pharmacology and Molecular Sciences, SoM, Final Oral, 2000

Francine Katz, PhD, Biochemistry/MolecularBiology, SPH, Thesis Reader/Alternate, 2000

Julie Goodman, ScM, Epidemiology, SPH, Thesis Reader, 2000

Kelly Young, PhD, Biochemistry/Molecular Biology, SPH, Final Oral, 2000

Debbie Drazen, PhD, Psychology, Arts and Sciences, Final Oral, 2001

Deborah Duffy, PhD, Psychology, School of Arts and Sciences, Final Oral, 2001

Matthew Portnoy, PhD, Biochemistry/Molecular Biology, SPH, Alternate, 2001

Maia Moraine, ScM, Biochemistry/Molecular Biology, SPH, Thesis Reader, 2001

Andrew Sobering, PhD, Biochemistry/Molecular Biology, SPH, Alternate, 2001

William Hanna, PhD, Biochemistry/Molecular Biology, SPH, Alternate, 2002

Liying Zhang, PhD, Biochemistry/Molecular Biology, SPH, Alternate, 2003

Staci D'Ann Bilbo, Psychological and Brain Sciences, School of Arts and Sciences, Final Oral, 

   2003

Mathew Show, PhD, Biochemistry/Molecular Biology, SPH, Final Oral, 2004

 

Classroom Instruction (current)

     Primary Instructor

Reproductive Physiology, School of Arts and Sciences

Multidisciplinary Studies in Reproduction Research, School of Public Health

     Participant

Introduction to the Biomedical Sciences (260.600), School of Public Health

Public Health Biology (550.630), School of Public Health

 

II.B       RESEARCH GRANT PARTICIPATION

a.  Previous Research Support

Correlation of basic protein composition and fine structure in differentiating spermatid and

mature sperm nuclei.  NSF GB 38787 and GB 41316, 1973-1976.  Principal Investigator.

Direct costs: $25,000.

Studies of a sulfhydryl oxidase from the male reproductive tract.  Ford Foundation 770-0314,

1977.  Co-Principal Investigator. Direct costs: $3600.

Release of DNA template restrictions in rabbit sperm nuclei.  Biomedical Research Support

            Grant BRSG H.32.6110, 1977.  Principal Investigator. Direct costs: $3600.

Spermatogenesis and fertilization in mammals.  NIH HD0992l, 1976-1979. Principal

Investigator. Direct costs: $160,000.

Programmatical studies on benign prostatic hyperplasia.  NIH AM 19300, 1976-1980.  Co-

Investigator. Direct costs: $90,000.

Early events in mammalian fertilization.  NSF PCM 7910675, 1979-1981.  Principal

Investigator. Direct costs: $35,000. 

Spermatogenesis and fertilization in mammals.  NIH HD09921, 1979-1983. Principal

Investigator.  Direct costs: $300,000.

Biological effects of exposure to environmental agents.  NIH ES 02277, 1980-1983.  Co-

Investigator. Direct costs: $54,000.

  Programmatical studies on benign prostatic hyperplasia, NIH AM19300, 1980-1984.  Co-

Investigator.  Direct costs: $100,000.

Spermatogenesis and fertilization in mammals.  NIH HD 09921, 1983-1988. Principal                        Investigator.  Direct costs: $335,000. 

Methods to assess male reproductive tract function.  U.S. EPA CR-812765-01-0, 1985-1987.

Principal Investigator. Direct costs: $210,000.

Programmatical studies on benign prostatic hyperplasia.  NIH AM 19300, 1984-1989.  Co-

Investigator.  Direct costs: $140,000.

Regulation of androgen secretion in mammalian testes.  NIH HD07204, 1986-1990.  Co-

Investigator. Direct costs: $40,000.

Rodent Studies on Cosmos-89.  NASA, 1989-1990.  Co-Investigator.  Direct costs: $12,000.

Small Instrumentation Award, Sterilizer.  BRSG, 1991.  Principal Investigator. Direct costs:

$15,000.

Testicular Perfusion, Leydig Cell Toxicants and Spermatogenesis.  EPA CR-816956, 1989-1993.    Project Manager.  Direct costs: $210,000.

Programmatical Studies on Benign Prostatic Hyperplasia.  NIH P01 DK19300, 1989-1994.  Co-

Investigator.  Direct costs: $400,000.

Aging and Male Reproductive Tract Structure and Function.  NIH P01 AG08321, 1989-1994.

Principal Investigator.  Direct costs: $400,000. 

Multidisciplinary Training Grant in Male Reproduction.  NIH T32 HD07276, 1990-1995.

Principal Investigator.  Direct costs: $525,000

Regulation of Androgen Secretion in Mammalian Testes.  NIH, 1990-1995. Principal

Investigator.  Direct costs: $650,000.

Testicular Perfusion, Leydig Cell Toxicants and Spermatogenesis.  EPA, 1989-1993. Principal

Investigator.  Direct costs: $210,000.

Aging and Male Reproductive Tract Structure and Function.  NIH P01 AG08321, 1994-1997.

Principal Investigator.  Direct costs: $1,550,000.

Multidisciplinary Training in the Reproductive Sciences.  NIH T32 HD07276, 1995-2000.

Principal Investigator.  Direct costs: $650,000.

Regulation of Spermatogenesis in Mammals.  NIH R01 HD09921, 1988-1994.  Principal                Investigator.  Direct costs: $382,000.

Hopkins Population Center.  NIH P30 HD06268, 1992-1997. Associate Director of Center;  and    Director, Core EM Laboratory. Direct costs: $280,000.

Regulation of Androgen Secretion in Mammalian Testes.  NIH RO1 HD07204, 1990-1995.

            Principal Investigator (after Dr. L. Ewing's death in August, l990).  Direct costs:

$650,000.

Hopkins Population Center.  NIH P30 HD06268, 1997-2002. Associate Director of Center; and     Director, Core EM Laboratory. Direct costs: $2,000,000

Training in the Cellular & Molecular Bases of Aging.  NIH, 1996-2002.  Principal Investigator.

Direct costs: $565,000.

Male Infertility and Its Biological Basis.  NIH, 1998-2004.  Principal Investigator. $3,750,000 

Atrazine Effects on the Male Reproductive Tract.  Novartis/Syngenta, 1998-2004.  Principal

            Investigator.  $500,000.

 

b.  Current Research Support

Aging and Leydig Cell Function.  AG21092, National Institute on Aging, NIH, 2002-2006.

   Principal Investigator. Direct costs: $1,100,000

Intratesticular Testosterone and Spermatogenesis in Man. National Institute of Child Health and    Human Disease, NIH, 2003-2008.  Principal Investigator. Direct costs: $1,300,000

Multidisciplinary Training in the Reproductive Sciences.  NIH T32 HD07276,  2000-2005.

   Principal Investigator. 3 predoc/1 postdoc slots

 

c.  Pending Research Support

Fetal Origins of Male Reproductive Diseases. NICHD, NIH.  Co-Investigator (P.I. Vassilios  Papadopoulos, Georgetown University).  Direct costs: $1,900,000

A Regulatory Web: Kit Ligand, GDNF and Spermatogonia.  NICHD, NIH.  Co-Investigator (P.I. W.W. Wright).  Direct costs: $1,250,000

Stem Cells in Testicular Development and Adult Function.  NICHD, NIH.  1 U54 HD049577-01

B. R. Zirkin, Principal Investigator. Direct costs: $5,300,000; total direct costs: $7,700,000

 

   This is a U54 application (Specialized Cooperative Centers in Reproduction Research) involving John Gearhart and Peter Donovan (Hopkins Institute for Cell Engineering), Vassilios Papadopoulos (Georgetown University), Matthew Hardy (Population Council), Erwin Goldberg (Northwestern University), and William Wright and Barry Zirkin (Hopkins School of Public Health).

Leydig Cell Stem Cell Development and Differentiation.  NICHD, NIH.  Co-Investigator (P.I.

   Matthew Hardy, Population Council, NY). Direct costs: $1,000,000

           

II. C      ACADEMIC SERVICE

School of Public Health

Member, Scholarships and Fellowships Committee, 1976-1977

Member, Steering Committee for Accreditation Self-Study, 1977-1979

Chairman, Accreditation Review Committee, Division of Biophysics, 1978

Chairman, Accreditation Review Committee, Department of Biochemistry, 1978

Member, Committee on Space Allocation, 1979-1980

Chairman, Biomedical Research Support Grant Subcommittee, 1979-1980

Member, Committee on Animal Laboratory Medicine, 1980-1981

Member, Committee on Appointments and Promotions, 1981-1984

Chairman, Department of Biochemistry Review Committee, 1983-1984

Chairman, Academic Ethics Board, 1984-1990

Member, Committee of the Whole, 1984-1987

Member, Advisory Board, 1984-1987

Member, Patents Committee, 1985-1987

President, Faculty General Assembly, 1985-1986

Member, Biotechnology Committee, 1986-1988

Chairman, Curriculum Committee, 1987-1988

Member, Physiology Division Review Committee,  Department of Environmental Health       Sciences, 1989

Chairman, Search Committee for Chair, Department of Biochemistry, 1989-1990

Chairman, Curriculum Committee, 1989-1991

Member, Search Committee for Chief Administrative/Financial Officer, 1990-1991

Chairman, Ad Hoc Committee on Governance, Faculty General Assembly, 1990- 1991

Member, Academic Ethics Board, 1991-1994

Member, Ad Hoc Committee, Review of Center for Alternatives to Animal Testing (CAAT),

1991-1993

Member, Ad Hoc Committee on Tenure, 1991-1992

Member, Strategic Planning Subcommittee on Research, 1992-1995

Member, Classroom Infrastructure Review Committee, 1992

Member, Appointments and Promotions Committee, 1993-1996

Member, Laboratory Sciences Review Committee, 1994-1995      

Member, Committee on Educational Innovation and Distance Learning, 1994-1996

Member, Subcommittee on Master of Health Science Programs, 1995-1996

President, Faculty Senate, 1997-1998

Chairman, Committee on Laboratory Sciences in Public Health, 1997-1998

Member, Steering Committee, Council on Education for Public Health Self-Study,  1998-1999

Member, Advisory Board, 1996-1999

Member, Committee of the Whole, 1996-1999

Member, Committee on Finance, 1997-1999

Member, Interdisciplinary Public Health Graduate Program Committee, 1997-1998

Member, Committee on Use and Ownership of Educational Materials, 1997-2001     

Member, Subcommittee on School Environment, 1999-2001

Member, Public Health Advisory Committee, 2002-2003

Member, Committee on Appointments and Promotions, 2003-

 

Johns Hopkins University

Director, Core Electron Microscopy Laboratory, The Johns Hopkins University Population

            Center, 1978-

Member, Johns Hopkins University Biomedical Scholars Selection Committee (Pew, Searle,

            Markey), 1989-1993

Associate Director, The Johns Hopkins University Population Center, 1990-1992

Member, Johns Hopkins University Population Committee, 1991- 1998

Member, Health Professions Recommendation Committee (School of Arts and Sciences), 1996-

   present

Director, Specialized Cooperative Center in Reproduction Research, 1998-2004

Member, Undergraduate Public Health Major Studies Program Advisory Committee, 2000-2001.

Alternate Member, Institutional Animal Care and Use Committee (IACUC), 2002-2004.

 

 

II.D       REPRESENTATIVE INVITED PRESENTATIONS (1995-present; selected)

Male Reproductive Toxicity, U.S. Environmental Protection Agency, 1995

Spermatogenesis,  Food and Drug Administration,  Center for Drug Evaluation and Research,

1995

Leydig Cell Aging, Bayview Medical Center, Asthma and Allergy Center, 1995

The Mechanism of Rodent Leydig Cell Hyperplasia and Tumor Formation and Its Relevance to

Human Health, American Industrial Health Council,  Leesburg, VA, 1995

Hormonal and Molecular Regulation of Spermatogenesis,  SUNY Binghamton, 1995

Male Reproductive Aging, International Congress of Endocrinology, San Francisco, CA, 1996

Physiology and Development of Male Reproductive Tract, Gordon Research Conference,

Plymouth, NH, 1996

Aging and Testicular Function,  Third International Symposium on Neurobiology and

Neuroendocrinology of Aging, Bregenz, Austria, 1996

Reproductive Endpoints for Human Health Risk Assessment: Circulating Hormones,

International Life Sciences Institute (ILSI) Workshop, 1997

Animal Models of Aging in Male: The Brown Norway Rat as a Model of Ageing, Vith

            International Congress of Andrology, Innternational Society of Andrology,  Salzburg,

Austria, 1997

Male Reproductive Aging,  Keynote address,  Canadian Fertility and Andrology Society, 1997  

The Male Reproductive System and Aging, The Endocrine Society, 1997

Male Reproductive Aging, American Society of Andrology, Los Angeles, 1998

Animal Models of Aging Males: Symposium on Clinical Consequences of Partial Androgen

Deficiency in Aging Men, First World Congress on The Aging Male, Geneva,

Switzerland, 1998

Molecular Regulation of Leydig Cell Aging, Charles R. Drew University of Medicine and

Science, Los Angeles, 1998

Aging of the Male Reproductive Tract, Society for the Study of Reproduction, Washington State     University, August, 1999

Regulation and Consequences of Leydig Cell Aging, Dean's Lecture, Johns Hopkins School of   Hygiene and Public Health, 1999

The Revolution in Assisted Reproductive Technologies: Do we know what we are doing?

Coffeehouse Colloquium, Johns Hopkins University Montgomery County Center, 1999

Regulation and Consequences of Leydig Cell Aging, National Institute on Aging Summer

Institute on Aging Research, 2000

Leydig Cell Aging: Regulation and Consequences, University of Pennsylvania, 2002

Leydig Cell Aging: Regulation and Consequences,  Deparement of Gynecology and Obstetrics,

            Johns Hopkins School of Medicine, 2002

Aging of the Male Reproductive Tract, University of Washington, 2002

Aging of the Male Reproductive Tract, Delaware State University Minority Access to Research     Careers (MARC) Program, 2002

Atrazine and Male Reproduction, Association of Government Toxicologists, NIH, 2002

Aging and Male Reproduction, Georgetown University Medical Center, 2002

Male Reproductive Aging: Mechanisms and Consequences, U.S. Environmental Protection

            Agency, 2003

Male Reproductive Aging: Mechanisms and Consequences, Kronos Longevity Institute

            (Phoenix), 2003

Male Reproductive Aging, Summer Institute on Aging Research (Arlington, VA), 2003

Human Reproductive Ageing: Insights Into Mechanisms from an Animal (Rat) Model,

Symposium on Reproductive Consequences of Ageing, 3rd Joint Meeting of British Andrology Society, British Fertility Society and Society for Reproduction and Fertility, University of Aberdeen, 2003

Aging and Male Reproduction: What Happens? Why Does it Happen? Does it Matter?

            Binghamton University, 2003.

Testosterone Production and the Aging Male: Regulation and Consequences, Center for

            Reproductive Science, Northwestern University, 2003.

Male Reproductive Aging:  Mechanisms and Consequences, The Jackson Laboratories, 2003

Male Contraception: From Rat to Man, Conference on Future of Male Contraception, National Institute of Child Health and Human Development, Seattle, 2004.

Male Reproductive Aging:  Mechanisms and Consequences, Conference on A New Paradigm for Aging Research: When Do the Biological Changes of Aging Begin? National Institute on Aging, Bethesda, 2004

Leydig Cell Aging:  Mechanisms and Consequences, University of Texas Health Sciences Center, San Antonio, 2004

Atrazine:  Effects of Gestational Administration on Fetal, Neonatal, Pubertal and Adult Testes.  Syngenta, Savannah, Georgia, 2004

 

LA NEW PARADIGM FOR AGING RESEARCH: 

WHEN DO THE BIOLOGICAL CHANGES OF AGING BEGIN?

II.E       ADDITIONAL INFORMATION

Research and Research Objectives

    A major research focus in our lab, supported by a RO1 grant from the National Institute on Aging, NIH, is on understanding how Leydig cells, the testosterone-producing cells of the mammalian testis, age. Current studies are designed to characterize the Leydig cell deficits that result in the reduced production of testosterone that occurs with aging,  and to understand the mechanisms by which aging Leydig cells become steroidogenically hypofunctional.  With respect to the latter, we are examining the possibility that intrinsic changes in the Leydig cell itself, rather than age-related changes that extrinsic to Leydig cells, are responsible for changes in Leydig cell function.  The generation by the Leydig cell of reactive oxygen species, a product of steroidogenesis itself, appears to be a good candidate to explain age-related reductions in steroidogenesis.  Indeed, evidence gathered to date suggests that the suppression of steroidogenesis by the administration of a hormonally-based  male contraceptive has the unexpected additional effect of suppressing Leydig cell aging.  This work also has significant implications for understanding, and perhaps preventing, deficits resulting from reduced testosterone in the aging male.  Planned studies involve translational research on the aging human male.

 

     One second research focus is on the endocrine regulation of mammalian spermatogenesis, supported by an RO1 grant from the National Institute on Child Health and Human Development, NIH. The studies are being conducted both in man and in an animal model (rat).   Morphological, biochemical and molecular methods are being used to examine the responses of testicular germ cells to changes in the intratesticular hormonal environment to which they are exposed.  In particular, germ cell apoptotic death and proliferation in response to contraceptive and higher doses of androgen (testosterone), FSH and growth factors are being examined.  Molecular studies are designed to explain how testosterone functions to maintain germ cells in the adult testis, and to restore germ cells to the testis subsequent to the administration of contraceptive doses of hormones to animals. Parallel studies are being conducted in men, in collaboration with Dr. Jonathan Jarow, Department of Urology.  These studies have been designed based on the animal studies.  We are studying the role of testosterone in human male infertility and in male hormonal contraception.

 

     A third focus is on the effects of environmental toxicants on the male reproductive tract.  In this regard, we are studying atrazine, a widely used herbicide that replaced DDT in many parts of the world, and on phthlates.  Preliminary evidence suggests that ingested atrazine and phthalates, when administered either during gestation or during the peripubertal period, affect testosterone production. The company that manufactures atrazine, Novartis (now Syngenta Crop Protection), has been funding the atrazine study.   NIH funding is being sought for the phthalate studies.

 

2. Keywords

reproductive biology, testis, spermatogenesis, Leydig cell, contraception, reproductive toxicology