September 8, 2004

Sidney Ray Grimes, Jr., Ph.D.

 

Work Address:            (VA Medical Center)                                    (LSU Health Sciences Center)

Research Service (151) Department of Biochemistry

                                    Overton Brooks VA Med Center      and Molecular Biology

                                    510 E. Stoner Ave.                              1501 Kings Highway, PO Box 33932

                                    Shreveport, LA 71101-4295                 Shreveport, LA 71130-3932

 

Telephone:                  318-211-8411 ext. 6685

FAX:                             318-429-5734

e-mail:                          sgrime@lsuhsc.edu, Sidney.Grimes@va.med.gov, and srgrimes@prysm.net

Education:                   University of North Carolina, Chapel Hill,

                                                North Carolina (Chemistry) B.S., 1969

                                    University of North Carolina, Chapel Hill,

                                                North Carolina (Biochemistry) Ph.D., 1973

                                    University of Texas, Houston, Texas

                                                (Postdoctoral Research, Biochemistry) 1973-1975

                                    Vanderbilt University, Nashville, Tennessee

                                                (Postdoctoral Research, Biochemistry) 1975-1976

 

Academic and Professional Experience:

 

            1997-present   Research Professor of Biochemistry and

                                    Molecular Biology, Louisiana State University

                                    Health Sciences Center and Research Chemist, VA Medical

                                    Center, Shreveport, Louisiana.       

            1/1999-3/2000            Acting ACOS for Research and Development, VA Medical Center

            1988-1997            Research Associate Professor of Biochemistry and

                                    Molecular Biology, Louisiana State University

                                    Health Sciences Center and Research Chemist, VA Medical

                                    Center, Shreveport, Louisiana.

            1984                Visiting scientist for two months in the laboratory

                                    of Drs. Gary and Janet Stein, Department of

                                    Biochemistry and Molecular Biology, University of

                                    Florida, Gainesville, Florida.

            1976-1988            Assistant Professor of Biochemistry and Molecular

Biology, Louisiana State University Health Sciences Center, and

Research Chemist, VA Medical Center, Shreveport, Louisiana

            1975-1976            Research Associate, Vanderbilt University,

                                    Nashville, Tennessee

            1974-1975            Research Associate,  University of Texas System

                                    Cancer Center, M.D. Anderson Hospital and Tumor Institute

            1973-1974    Project Investigator, Department of Biochemistry,

                                    The University of Texas System Cancer Center, M.D.

                                    Anderson Hospital and Tumor Institute

            1969-1973            Research Assistant in Biochemistry, University of

                                    North Carolina, Chapel Hill, North Carolina

 

 

 

Awards:                        A National Research Service Award sponsored by the U.S.

                                    Public Health Service administered by Vanderbilt

                                    University Reproductive Biology, Biochemistry and

                                    Endocrinology. Cited in American Men and Women of Science

                                    Cited in Marquis Who's Who in Science and Engineering

 

Fields of Interest:             Regulation of gene expression. The role of nuclear proteins in

regulating transcription during cellular differentiation and

development.

 

Scientific Societies:            American Society for Cell Biology - Active

                                    Sigma Xi - Active

                                    American Society for Microbiology - Active

                                    American Assoc. for the Advancement of Science - Active

                                    Society for the Study of Reproduction - Active

 

Teaching Experience:

 

            Biochemistry- Supervised three dental students in Biochemistry research project,

                                    Fall, 1972 and Spring, 1973,  Univ. North Carolina.

 

            Developmental Biology Course - Graduate student lectures, Dept. Biochemistry,

                                    Univ. TX, 1973.

 

            Biochemistry of Nuclear Proteins Course - Graduate student lectures, 1975.

 

            Biochemistry- Lectures and laboratory in the medical Biochemistry course

                                    in the Dept. Biochemistry and Molecular Biology, 1976 - 1984.

                                    Graduate Biochemistry course lectures (1993 - present), (13 hr 2002)

                                    LSUHSC,  Shreveport.

 

            Nucleic Acids course.  Two-thirds of lectures in this one

                                    semester graduate course,  Dept. Biochemistry

                                    and Molecular Biology, LSUHSC - Shreveport, spring, 1980.

 

            Cell Biology- Lectured in this interdepartmental graduate course

                                    in the Dept. Biochemistry and Molecular Biology,

                                    LSUHSC, Shreveport, 1984.

 

            Biochemical Analytical Methods -  Presently lecture in this             graduate course.

                                    Alternate years (1989 - 1993), Every year (4 hr/yr; 1994 - present).

                                    Dept. Biochem. and Molec. Biol., LSUHSC., Shreveport.                   

 

 

Editorial Boards:            Molecular Biology Reports  (Kluwer Academic publishers)            1996 - Present

                                    Reproductive Biology and Endocrinology (BioMed Central Assoc.

                                                Electronic Journals) 2003 - Present.

                                    Biology of Reproduction July, 2003 - present.

 

 

 

Committees:                

 

 

                                    (VA Medical Center)

 

            Research and Development Committee                   -            1977 - 1980

            Research Evaluation Subcommittee                          -            1977 - 1980

            Radioisotope Committee                                           -            1977 - 1980

            Subcommittee on Animal Studies                         -            1977 - 1987

            Subcommittee on Animal Studies                         -            1993 - 1998

            Subcommittee on Research Safety                          -            1980 - Present

            Chairman, Subcommittee on Research Safety  -            1983 - Present

            Chairman, Subcommittee on Animal Studies                        -            1980 - 1987  

            Chairman, Subcommittee on Animal Studies                        -            1993 - 1998  

            Chairman, Research Biosafety Subcommittee            -            1985 - 1994

            Research Biosafety committee                                -            1994 - Present

            Subcommittee on Human Studies                                    -            1990 - 1991

            Subcommittee on Human Studies                                    -            1993 - 1995

            VA Medical Center Safety Committee                   -            1996 - 1967

            VA Radiation Safety Committee                               -             1996 - Present

            VA Hazardous Materials Subcommittee              -            1996 - Present

            Research and Development Committee                   -            1999 - 2000

            Research and Development Committee                   -            2002 - Present

            Medical Library Advisory Committee                   -            1999 - 2000

            VISN 16 Data Privacy Board                                      -            2002 - Present

           

 

 

                                    (LSU Health Sciences Center)

 

            Elected Faculty Council                                                -            1988 - 1990  

            Research Advisory Committee

                        for ACS and Cancer Center Grants:                        -            1992

            Research Advisory Committee (Peer Review grants)-            1993 -1998

            Faculty Search Committee                                           -            1994

            Graduate Student Admissions Committee                   -            1994 - 1997

            Graduate Student Admissions Committee                   -            1998 - 2001

            Biosafety Committee            (and RAS Member)              -            1995 – 6/1999

            Biosafety Committee            (RAS Member only)              -            6/1999

            Departmental Library Committee                               -            2001 - present

            Animal Care and Use Committee                               -            2003 - present

 

 

 

 

                                    (Graduate Student Committees)

                       

                        Biochemistry and Molecular Biology

 

                                    Marcie Fowler, Ph.D.               2002- present

                                    Kenneth R. Noss, Ph.D.   1998-2002 Major Advisor (Grad. 8/02)

                                    Donald C.Wilkerson, Ph.D.   1998-2002 Major Advisor (Grad. 8/02)

                                   Kevin P. Pavlick, Ph.D            1996-2000  (Graduated 12/00)

                                    Claudia Orellana, M.S.            1999-2000               

                                    Heather Panek, Ph.D.             1994-1999  (Graduated 6/99)

                                    Micheal Wheeler, Ph.D.            1998-1999      

                                    Donald C.Wilkerson, M.S.     1996-1998 Major Advisor (Grad.  6/98)

                                    Peter Mottram         , M.S.     1996-1998 Major Advisor (Grad.12/98)

Jane vanWert, Ph.D. 1994-1997 Major Advisor (Grad. 6/97)

Guofeng Xie, Ph.D. 1991-1995 (Graduated 6/95)

                                    Cletus D'Souza, Ph.D.             1991-1995    ”

                                    Inez Pinto, Ph.D.                 1988-1992    ”

                                    Christopher Adams, Ph.D.   1988-1992    ”

                                    Jong Na, Ph.D.                     1988-1992    ”

                                    Dan Ware, M.S.                   1988-1991    ”

 

                        Microbiology and Immunology

           

                                    Aaron Martin, Ph.D.                2002- present

                                    Shu-Jan Lee, Ph.D.                         1987               

                                    Susan A. Key, M.S.                 1985               

                                    Thomas F. Bittick, M.S.     1985               

                                    Lawton A. Seal, Ph.D.             1985               

           

                        Medical Students

           

                                    Jeffrey Anderson                     1988-1990

                                    Philip Andrew Rascoe            1997 (Am.Can.Soc.Research

                                                                                                Fellowship Recipient)

 

Graduate Student Rotations

                       

                                    Kenneth Noss                         1997-98 (Jan-March)

                                    Micheal Wheeler                       1997       (May-July)

                                    Peter Mottram                                   1997       (March-June)

                                    Donald C. Wilkerson                1995-96 (December-March)

                                    Peggy Merchant                     1994       (March-June)

                                    Heather Panek                                    1994       (March-June)

                                    Jane vanWert                                   1993       (June-Aug.)

 

Postdoctoral Fellows Trained in my laboratory -   Steven A. Wolfe, Ph.D.

                               -   Jane vanWert,   Ph.D.

Currently mentor for Rakesh Singal, M.D.    1998-present

                        Dr. Singal has received four funded grants during my service as his

                        mentor: a VA Merit Review Grant (active), a DOD grant (active 2002),

                        an NIH grant (active 2002), and a VA Career Development Award (2002).

            I have agree to mentor Subhashini Yaturu, M.D. 2004 - present

 

 

 

Publications:              (Full Length Papers)

 

1.         S.R. Grimes, 1973.  "Studies on Rat Testis Histones," Title of Thesis for Ph.D. in Biochemistry, University of North Carolina, Chapel Hill, North Carolina.

 

2.         S.R. Grimes, C-B. Chae, and J.L. Irvin, 1975.  "Acetylation of Histones of Rat Testis." Archives of Biochemistry and Biophysics. 168: 425-435.

 

3.         R.E. Branson, S.R. Grimes, G. Yonuschot, and J.L. Irvin, 1975."The Histones of Rat Testis." Archives of Biochemistry and Biophysics. 168: 403-412.

 

4.         S.R. Grimes, C-B. Chae, and J.L. Irvin, 1975.  "Effects of Age and Hypophysectomy Upon Relative Proportions of Various Histones in Rat Testis."  Biochem. Biophys. Res. Comm. 63: 911-917.

 

5.         S.R. Grimes, R.D. Platz, M.L. Meistrich, and L.S. Hnilica, 1975. "Partial Characterization of New Basic Nuclear Protein from Rat Testis Elongated Spermatids."  Biochem. Biophys. Res. Comm. 67: 182-189.

 

6.         R.D. Platz, S.R. Grimes, M.L. Meistrich, and L.S. Hnilica, 1975. "Changes in Nuclear Proteins of Rat Testis Cells Separated by Velocity Sedimentation." J. Biol. Chem. 250: 5791-5800.

 

7.         S.R. Grimes, M.L. Meistrich, R.D. Platz, and L.S. Hnilica, 1977. "Nuclear Protein Transitions in Rat Testis Spermatids." Expt'l Cell Res. 110: 31-39.

 

8.         M.L. Meistrich, S.R. Grimes, R.D. Platz, W.A. Brock, and L.S. Hnilica, 1977.  "Molecular Events in Spermatogenesis." The Cancer Bulletin. 29: 12-13.

 

9.         M.L. Meistrich, W.A. Brock, S.R. Grimes,  Jr., R.D. Platz, and L.S. Hnilica, 1978.  "Nuclear Protein Transitions During Spermatogenesis." Federation Proceedings 37: 2522-2525.

 

10.       M.L. Meistrich, J.L. Longtin, W.A. Brock,  S.R. Grimes, and M.L. Mace, 1981.  "Purification of Rat Spermatogenic Cells and Preliminary Biochemical Analysis of These Cells." Biol. of Reproduction 25: 1065- 1077.

 

11.       S.R. Grimes, Jr. and N. Henderson. 1983. "Acetylation of Histones During Spermatogenesis in the Rat" Arch. Biochem. Biophys. 220:108-116.

 

12.       S.R. Grimes, Jr. and N. Henderson. 1984. "Acetylation of Rat Testis Histones H2B and TH2B." Developmental Biology 101:516- 521.

 

13.       S.R. Grimes. 1984. "Release of Nuclear Ribonucleoprotein During Brief Digestion of Rat Testis Chromatin with Deoxyribonuclease II (3.1.4.6)" Compar. Biochem. Biophys. 78B:633-641.

 

14.       S.R. Grimes, Jr. and N. Henderson. 1984. "Hyperacetylation of Histone H4 in Rat Testis Spermatids." Exp. Cell Res. 152:91- 97.

 

15.       S.R. Grimes, Jr. and P. Smart, 1985. "Changes in the Structural Organization of Chromatin During Spermatogenesis in the Rat." Biochim. Biophys. Acta. 824:128-139.

 

16.       S.R. Grimes, Jr., 1986. "Nuclear Proteins in Spermatogenesis." Compar. Biochem. Biophys. 83B:495-500.   

 

 

 

 

 

Publications:                (Full Length Papers)

 

17.       P. Weisz-Carrington and S.R. Grimes, Jr., and M.L. Lamm, 1987. "Gut Associated Lymphoid Tissue as A Source of an IgA Immune Response in Respiratory Tissues After Oral Immunization and Intrabronchial Challenge." Cellular Immunology. 106:132-138.

 

18.       S.R. Grimes, P. Weisz-Carrington,  H.  Daum,  J.  Smith,  L.  Green, K. Wright, G.S. Stein, and  J.L. Stein, 1987. "A Rat Histone H4 Gene Closely Associated  with the  Testis-Specific  H1t  Gene." Exptl. Cell Res. 173:534-545.

 

19.       S.A. Wolfe, J.V. Anderson, S.R. Grimes, G.S. Stein, and J.L. Stein, 1989. "Comparison of the Structural Organization and Expression of Germinal and Somatic Rat Histone H4 Genes."  Biochem. Biophys. Acta 1007:140-150.

 

20.       S.R. Grimes, S.A. Wolfe, J.V. Anderson, G.S. Stein, and J.L. Stein,  1990. "Structural and Functional Analysis of the Rat Testis-Specific Histone H1t Gene." J. Cellular Biochem. 44:1-17.

 

21.       T.A. Owen, J.Holthuis, E.Markose, A.J. van Wijnen, S.A. Wolfe, S.R. Grimes, J.B. Lian, and G. Stein, 1990 "Modifications of Protein-DNA Interactions in the Proximal Promoter of a Cell Growth Regulated Histone Gene During the Onset of Osteoblast Differentiation." PNAS USA 87:5129 - 5133. (Communicated by David Prescott).

 

22.       S.A. Wolfe and S.R. Grimes, 1991. "Protein-DNA Interactions within the Rat Histone H4t Promoter." J. Biol. Chem. 266:6637-6643.

 

23.       S.R. Grimes, S.A. Wolfe, and D.A. Koppel, 1992. "Tissue-Specific Binding of Testis Nuclear Proteins to a Sequence Element within the Promoter  of the Testis-Specific Histone H1t Gene."  Arch. Biochem. Biophys. 296:402-409.

 

24.       S.R. Grimes, S.A. Wolfe, and D.A. Koppel, 1992. "Temporal Correlation between the Appearance of Testis-Specific DNA-Binding Proteins and the Onset of Transcription of the Testis-Specific Histone H1t Gene."  Exptl. Cell Res. 201:216-224.

 

25.       M.W. Owens and S.R. Grimes, 1993. "Pleural Mesothelial Cell Response to Inflammation: Tumor Necrosis Factor-Induced Proliferation and Collagen Synthesis." Amer. J. Physiology: Lung Cellular and Molecular Physiology 265:L382-L388.

 

26.       S.A. Wolfe and S.R. Grimes, 1993. "Histone H1t: A Tissue-Specific Model used to Study Transcriptional Control and Nuclear Function During Cellular Differentiation." J. Cell. Biochem. 53:156-160.

 

27.       S.R. Grimes, S.A. Wolfe, D.A. Koppel, and L. Fogelfeld. "Regulation of Expression of Mammalian Testis Histone Genes."   Trends in Comparative Biochemistry and Physiology, 1: (1993) pp.365-375.

 

28.       D.A. Koppel, S.A. Wolfe, L. Fogelfeld, P. Merchant, L. Prouty, and S.R. Grimes, 1994. "Primate Testicular Histone H1t Genes are Highly Conserved and the Human H1t Gene is Located on Chromosome 6" J. Cellular Biochemistry. 54:212-222.

 

29.       S.A. Milligan, M.W. Owens, R.J. Henderson, and S.R. Grimes, 1995. "Characterization of Proteoglycans Produced by Rat Pleural Mesothelial Cells in vitro." Exp. Lung. Res. 21:559-575.

 

30.       J. VanWert, S.A. Wolfe, and S.R. Grimes, 1995. "Testis-Specific Expression of the Rat Histone H1t Gene in Transgenic Mice".  Biochemistry 34:8733-8743.

 

Publications:                (Full Length Papers )

 

 

31.       S.A. Wolfe, vanWert, and S.R. Grimes, 1995. "Expression of the Testis-Specific Histone H1t Gene: Evidence for Involvement of Multiple Cis-acting Promoter Elements".  Biochemistry 34:12461-12469.

 

32.       J. VanWert, S.A. Wolfe, and S.R. Grimes, 1996. "Binding of Nuclear Proteins to a Conserved Histone H1t Promoter Element Suggests an Important Role in Testis-Specific Transcription". J. Cellular Biochemistry 60:348-362.

 

33.       S.R. Grimes, S.A. Wolfe, and J. vanWert, 1997. "Regulation of Transcription of the Testis-Specific Histone H1t Gene by Multiple Promoter Elements". Molecular Biology Reports 24:175-184.

 

34.       J.M. vanWert, H.R. Panek, S.A. Wolfe, and S.R. Grimes, 1998. "The TE Promoter Element of the Histone H1t Gene is Essential for Transcription in Primary Spermatocytes". Biology of Reproduction. 59:704-710.

 

35.       S.A. Wolfe and S.R. Grimes, 1999. "Binding of Nuclear Proteins to an Upstream Element Involved in Transcriptional Regulation of the Testis-Specific Histone H1t Gene".  J. Cellular Biochemistry 75:555-565.

 

36.       S.A. Wolfe, P.J. Mottram, J.M. vanWert and S.R. Grimes, 1999. "Localization of Upstream Elements Involved in Transcriptional Regulation of the Rat testis-Specific Histone H1t Gene in Somatic Cells". Biology of Reproduction 61:1005-1011.

 

37.       D. Good, P.O. Schwarzenberger, J.A. Eastham, R.E. Rhoads, J.D. Hunt, M. Collins, M. Batzer, C. Theodossiou, J.K. Kolls,  and S.R. Grimes, 1999. "Cloning and Characterization of the Prostate-Specific Membrane Antigen Promoter". J. Cellular Biochemistry 74:395-405.

 

38.       R. Singal, J.M. vanWert, M. Bashambu, S.A. Wolfe, D.C. Wilkerson and S.R. Grimes, 2000. "The Testis-Specific Histone H1t Gene is Hypermethylated in Nongerminal Cells". Biology of Reproduction 63:1237-1244.

 

39.       R. Singal and S.R. Grimes, 2001. "Microsoft® WordTM Macro for Analysis of Cytosine Methylation by the Bisulfite Deamination Reaction". BioTechniques  30(1): 116-120.

 

40.       S.R. Grimes, S.A. Wolfe and R. Singal, 2001. "Macro for Analysis of CpG and CpNG Methylation in Plants". BioTechniques  30(6): 1248.

 

41.       K.R. Noss, S.A. Wolfe, and S.R. Grimes, 2002. "Upregulation of Prostate Specific Membrane Antigen (PSMA)/Folate Hydrolase (FOLH1) Transcription by an Enhancer".  Gene 285: 247-256.

 

42.      K. Noss, R. Singal, and S.R. Grimes, 2002. "Methylation Status of the Prostate Specific Membrane Antigen (PSMA) CpG Island in Prostate Cancer Cell Lines". Anticancer Research 22: 1505-1512.

 

43.       D.C. Wilkerson, S.A. Wolfe, and S.R. Grimes, 2002. "The H1t/GC-box and H1t/TE1 element are essential for promoter activity of the Testis-Specific Histone H1t Gene". Biology of Reproduction 67: 1157-1164.

 

44.       D.C. Wilkerson, S.A. Wolfe, and S.R. Grimes, 2002. "Sp1 and Sp3 activate the testis specific histone H1t promoter through the H1t/GC-box".  J. Cellular Biochem. 86:716-725.

 

 

 

 

Publications:                (Full Length Papers )

 

45.       S.R. Grimes, D.C. Wilkerson, K.R. Noss and S.A. Wolfe, 2003. "Transcriptional Control of the Testis-Specific Histone H1t Gene". GENE 304:13-21.

 

46.       D.C. Wilkerson, S.A. Wolfe, and S.R. Grimes, 2003. "The TE2 and TE1 sub-elements of the testis-specific histone H1t promoter are functionally different".