De Yi LIU
Highest degree: Ph.D
(in medicine, University of Melbourne).
Academic position: Research Fellow
(Reproductive Biologist)
Present correspondence address:
Dr De Yi Liu,
Department of Obstetrics & Gynaecology, University of Melbourne,
Royal Women's
Hospital, 132 Grattan Street, Carlton, VIC 3053, Australia.
Phone:
61-3-9344-2042, Fax: 61-3-9347-1761,
Email: dyl@unimelb.edu.au
Academic & Employment Record
1977-1980 Diploma,
Clinical Pathology and Medical Science, Congqing Medical School, Congqing,
Sichuan, China.
1980-1985 Scientist,
Andrology Laboratory, Sichuan Family Planning Research Institute (World Health
Organisation Cooperation Centre), Chengdu, Sichuan, China.
1980-1981 Research
trainee, Medical Research Centre, Teaching Hospital, Sichuan Medical
University, Chengdu, Sichuan, China.
1984-1985 World
Health Organization Visiting Scholar, Medical Research Centre, Prince Henry's
Hospital, Monash University, Melbourne, Australia.
1986-1990 Ph.D
student. University of Melbourne, Australia.
1991-2004 Research
Fellow, University of Melbourne Department of Obstetrics & Gynaecology,
Royal Women's Hospital, Carlton,
VIC 3052, Australia.
Honorary Professional Positions:
World Health Organization
Consultant for “Andrology - Vas Occlusion Workshop” Hanoi,
Vietnam, 1993.
Associate
Editor of Human Reproduction (2002-2004), an international scientific journal published for
the European Society for Human Reproduction and Embryology.
Editorial
board member of Reproduction and Contraception (2004, Chinese scientific journal).
Reviewer for the following international scientific
journals:
Fertility
and Sterility, Human Reproduction, Molecular Human Reproduction, International
Journal of Andrology (1995-2001), Asia Journal of Andrology, Reproduction and
Contraception (Chinese)
Professional Memberships
Members of
the Australian Society for Reproductive Biology (ASRB) and Fertility Society of
Australia (FSA).
Publications--(Refereed
Scientific Journals)
1. Liu
DY, Clarke GN and Baker HWG. The effect of serum on motility of human
spermatozoa in culture. International
Journal of Andrology 9:109-117, 1986
2. Liu
DY, Cooper EJ and Baker HWG. Seminal transferrin: an index of Sertoli cell
function: Is it of clinical value? Clinical
Reproduction and Fertility
4:191-197, 1986
3. Liu
DY, Jennings MG and Baker HWG. Correlation between defective motility (asthenospermia)
and ATP reactivation of demembranated human spermatozoa. Journal of Andrology 8:349-353, 1987
4. Liu
DY, Elton RA, Johnston WIH and Baker HWG. Spermatozoal nuclear chromatin
decondensation in vitro. A test for sperm immaturity. Comparison with results
of human in vitro fertilisation. Clinical
Reproduction and Fertility 5:191-201, 1987
5. Liu
DY, Du Plessis YP, Nayudu PL, Johnston WIH and Baker WHG. The use of in vitro
fertilization to evaluate putative tests of human sperm function. Fertility and Sterility 49:272-277,
1988
6. Liu
DY and Baker HWG. The proportion of sperm with poor morphology but normal
intact acrosomes detected with Pisum Sativum agglutinin correlates with
fertilization in vitro. Fertility and
Sterility 50:288-293, 1988
7. Liu
DY, Lopata A, Johnston WIH and Baker HWG. A human sperm-zona pellucida binding
test using oocytes that failed to fertilize in vitro. Fertility and Sterility 50:782-788, 1988
8.
Liu DY, Lopata A, Johnston WIH
and Baker HWG. Human sperm-zona pellucida binding, sperm characteristics and in
vitro fertilization. Human Reproduction
4:696-701, 1989.
9.
Liu DY and Baker HWG.
Hypoosmotic sperm swelling test as a sperm function test. Fertility and Sterility 51:365-366, 1989.
10.
Liu DY, Clarke GN, Lopata A, Johnston
WIH and Baker HWG. A sperm-zona pellucida binding test and in vitro
fertilization. Fertility and Sterility
52: 281-287,1989.
11.
Baker HWG and Liu DY. New tests
of human sperm function and in vitro fertilization. Reproduction, Fertility and Development 1:179-182, 1989.
12.
Liu DY and Baker HWG. Inducing
the human acrosome reaction with a calcium ionophore A23187 decreases
sperm-zona pellucida binding with oocytes that failed to fertilize in vitro. Journal of Reproduction and Fertility
89:127-134, 1990.
13.
Liu DY and Baker HWG.
Relationships between human sperm acrosin, acrosomes, morphology and in vitro
fertilization. Human Reproduction.
5:298-303, 1990.
14.
O'Bryan MK, Baker HWG, Saunders
JR, Kirszbaum L, Walker ID, Hudson, P, Liu DY, d'Apice AJF and Murphy BF. Human
seminal clusterin (SP40,40). Isolation and characterization. Journal of Clinical Investigation.
85:1477-1486, 1990.
15.
Liu DY, Lopata A and Baker HWG.
Use of oocytes that failed to be fertilized in vitro to study sperm-oocyte
interaction: comparison of sperm-oolemma and sperm-zona pellucida binding, and
relationship with results of IVF. Reproduction, Fertility and Development.
2:641-650, 1990.
16.
Liu DY, Clarke GN and Baker HWG.
Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm
antibodies. Fertility and Sterility. 55:440-442,
1991.
17.
Liu DY, Clarke GN and Baker HWG.
Relationship between sperm motility assessed with the Hamilton-Thorn Motility
Analyzer and fertilization rates in vitro. Journal
of Andrology, 12:231-239, 1991.
18.
Liu DY, Lopata A, Pantke P and
Baker HWG. Horse and marmoset sperm bind to the zona pellucida of salt stored
human oocytes. Fertility and Sterility,
56:764-767, 1991.
19.
Liu DY and Baker HWG. Morphology
of spermatozoa bound to the zona pellucida of human oocytes that failed to
fertilize in vitro. Journal of
Reproduction and Fertility, 94:71-84, 1992.
20.
Ng FLH, Liu DY and Baker HWG.
Comparison of Percoll, mini-percoll and swim-up methods for sperm preparation
from abnormal semen samples. Human
Reproduction, 7:261-266, 1992.
21.
Liu DY and Baker HWG. Tests of
human sperm function and fertilization in vitro. Fertility and Sterility, 58:465-483, 1992.
22.
Liu DY and Baker HWG. Sperm
nuclear chromatin normality: relationship with sperm morphology, sperm-zona
pellucida binding and fertilization rates in vitro. Fertility and Sterility 58:1178-1184, 1992.
23.
Liu DY and Baker HWG. Inhibition
of acrosin activity with a Trypsin inhibitor blocks human sperm penetration of
the zona pellucida. Biology of
Reproduction 48:340-348, 1993.
24.
Baker HWG and Liu DY, Bourne H
and Lopata A. Diagnosis of sperm defects in selecting patients for assisted
fertilization. Human Reproduction, 8:1779-1780,
1993.
25.
Liu DY and Baker HWG. Tests of
sperm function. Fertility and Sterility,
59:698-699, 1993.
26.
Liu DY and Baker HWG. A new test
for the assessment of sperm-zona pellucida penetration: relationship with
results of other sperm tests and fertilization in vitro. Human Reproduction, 9:489-496, 1994.
27.
Liu DY and Baker HWG. Acrosome
status and morphology of human sperm bound to the zona pellucida and oolemma
determined using oocytes that failed to fertilize in vitro. Human Reproduction, 9:673-679,1994.
28.
Liu DY and Baker HWG. Disorderd
acrosome reaction of sperm bound to the zona pellucida (ZP). a newly discovered
sperm defect causing infertility with reduced sperm-ZP penetration and reduced
fertilization in vitro. Human
Reproduction, 9:1694-1994, 1994.
29.
O'Bryan MK, Murphy BF, Liu DY,
Clarke GN and Baker HWG. The use of anticlusterin monoclonal antibodies for the
combined assessment of human sperm morphology and acrosome integrity. Human Reproduction, 9:1490-1496, 1994.
30.
Clarke,GN, Liu DY and Baker HWG.
Immunoinfertility: a case study with implications for immunocontraception. Archives of Andrology, 35:21-27, 1995.
31.
Liu DY, Robyn Johnston R and
Baker HWG. Ability of sperm to bind to the zona pellucida during
oligozoospermia induced with testosterone during a male contraceptive trial. International Journal of Andrology 18
(Suppl 1):39-44, 1995 1995.
32.
Johnston RC, Clarke GN, Liu DY,
and Baker HWG. Assessment of the Sperm Quality Analyser (SQA). Fertility and Sterility, 63:1071-1076,
1995.
33.
Liu DY, Bourne H and Baker HWG.
Fertilization and pregnancy with acrosome intact sperm by intracytoplasmic sperm
injection in patients with disordered zona pellucida induced acrosome reaction.
Fertility and Sterility, 64:116-121,
1995.
34.
Bourne H, Liu DY, Clarke GN and
Baker HWG. Normal fertilization
and embryo development with an round headed acrosomeless sperm. Fertility and Sterility, 63:1329-1332, 1995.
35.
Pantke P, Hyland J, Galloway DB,
Liu DY and Baker HWG. Development of a zona pellucida sperm binding assay for
the assessment of stallion fertility. Biology
of Reproduction, Monograph 1 (Equine Reproduction VI):681-687, 1995.
36.
Bourne H, Richings N, Liu DY,
Clarke GN, Harari O and Baker HGW. Sperm preparation for intracytoplasmic
injection: methods and relationship to fertilization results. Fertility, Reproduction and Development,7:177-183,
1995.
37.
Liu DY and Baker HWG. A simple
method for assessment of the human acrosome reaction of spermatozoa bound to
the zona pellucida: lack of relatioship with ionophore A23187-induced acrosome
reaction. Human Reproduction,
11:551-557, 1996.
38.
Liu DY and Baker HWG. Relationship
between the zona pellucida (ZP) and ionophore A23187 induced acrosome reaction
and the ability of sperm to penetrate the ZP in men with normal sperm-ZP
binding. Fertility and Sterility,
66:312-315, 1996.
39.
Garrett C, Liu DY and Baker HWG.
Selectivity of the human sperm-zona pellucida bining process to sperm head
morphometry. Fertility and Sterility,
67, 362-371, 1997.
40.
Liu DY, Bourne H and Baker HWG.
High fertilization and pregnancy rates after intracytoplasmic sperm injection
in patients with disordered zona pellucida-induced acrosome reaction. Fertility and Sterility. 67, 955-958,
1997.
41.
Liu DY, Baker HWG, Pears MJ and
d Apice AJF. Normal sperm-zona pellucida interaction and fertilization in vitro
in a-1,3-galactosyltransferase gene knockout mice. Molecular Human Reproduction. 3, 1015-1016, 1997.
42.
Liu DY and Baker HWG. Protein
Kinase C plays an important role in human zona pellucida-induced acrosome
reaction. Molecular Human Reproduction,
3, 1037-1043, 1997.
43.
Liu DY and Baker HWG. Calcium
ionophore-induced acrosome reaction correlates with fertilization rates in
vitro in patients with teratozoospermic semen. Human Reproduction, 13:905-910, 1998.
44.
Liu DY, Martic M, Clarke GN,
Dunlop ME and Baker HWG. An important role of actin polymerization in the human
zona pellucida-induced acrosome reaction. Molecular
Human Reproduction, 5: 941-949, 1999.
45.
Liu DY and Baker HWG. Defective sperm-zona pellucida
interaction: a major cause of failure of fertilization in clinical in-vitro
fertilization. Human Reproduction,
15: 702-708, 2000.
46.
Baker HWG, Liu DY, C Garrett and
M Martic The human acrosome reaction. Asian
Journal of Andrology, 2: 172-178, 2000.
47.
Liu DY, Clarke GN, Martic M, Garrett C and Baker HWG.
Frequency of disordered zona pellucida (ZP)-induced acrosome reaction in
infertile men with normal semen analysis and normal sperm-ZP binding. Human Reproduction, 6: 1185-1190, 2001.
48.
Liu DY, Martic M, Clarke, GN, Grkovic I, Garrett C,
Dunlop ME and Baker HWG. An anti-actin monoclonal antibody inhibits the zona pellucida
induced acrosome reaction and hyperactivated motility of human sperm. Molecular Human Reproduction, 8, 37-47,
2002.
49.
Liu DY, Martic
M, Garrett C, Dunlop ME and Baker HWG. Phorbol myristate acetate induces
ruffling of the acrosome of human spermatozoa. Fertility and Sterility, 78: 128-136, 2002.
50.
Liu DY and Baker HWG. Disordered
zona pellucida induced acrosome reaction and failure of in vitro fertilization
in patients with unexplained infertility. Fertility
and Sterility, 79: 74-80, 2003.
51.
Liu, DY and Baker HWG. Frequency
of defective sperm-ZP interaction in severely teratospermic infertile men. Human
Reproduction, 18: 802-807, 2003.
52.
Liu DY, Stewart T and Baker HWG.
Normal range and variation of the zona pellucida-induced acrosome reaction in fertile
men. Fertility and Sterility, 80:
384-389, 2003.
53.
Garrett C, Liu DY, Clarke GN, Rushford, DD and Baker,
HWG. Automated semen analysis variables : zona pellucida preferred sperm
morphometry and straight line velocity, are related to pregnancy rate in
subfertile couples. Human Reproduction,
18: 1643-1649, 2003.
54.
Liu DY, Garrett C and Baker HWG. Low proportions of
spermatozoa can bind to the zona pellucida of human oocytes. Human Reproduction, 18: 2382-2389,
2003.
55.
Clarke GN, Liu DY and Baker HWG. Improved sperm
cryopreservation after very rapid cooling above the freezing point. Reproduction,
Fertility and Development, 15: 377-381, 2003.
56.
Liu, DY and Baker HWG. High frequency of defective
sperm-ZP interaction in oligozoospermic infertile men. Human
Reproduction, 19:228-233, 2004.
57.
Martic M, Moses E, Adams T, Liu DY, Gook D, Garrett
C, Dunlop ME, Baker HWG Recombinant human zona pellucida proteins ZP1, ZP2 and
ZP3 co-expressed in a human cell line. Asian
Journal of Andrology. 6: 3-13, 2004.
58.
Apedaile AE, Garrett
C, Liu DY Clarke GN, Johnston SA and Baker HWG. Flow cytometry and
microscopic acridine orange test: relationship with standard semen analysis. Reproductive BioMedicine Online. 8
(4):398-407, 2004.
59.
Liu DY, Zhu WJ and Baker HWG. Importance role of
assessment of sperm function in clinical management of infertility in IVF/ICSI.
Reproduction & Contraception (Chinese) 24 (4): 193-199, 2004.
60.
Liu DY, Garrett C
and Baker HWG. Clinical
application of sperm-oocyte interaction tests in in vitro fertilization-embryo
transfer and intracytoplasmic sperm injection programs. Fertility and Sterility,
In press 2004.
61.
Liu DY, Clarke GN and Baker HWG. Exposing sperm surface actin epitopes
during vitro culture relates to sperm functions and capacitation in vitro. Human Reproduction Submitted 2004.
62.
Apedaile AE, Garrett
C, Liu DY Clarke GN, Bourne H, Johnston SA and Baker HWG. Does sperm DNA assessment by the flow
cytometry acridine orange test predict ART outcome? Reproductive BioMedicine Online. Submitted 2004.
Publications
(Book Chapters)
1 Baker
HWG, Ng FLH and Liu DY. Preparation and analysis of semen for IVF/GIFT. In Handbook of In Vitro Fertilization. (1st
Edition) Trounson A. O. and Gardner D.K., (eds), CRC press, Boca Raton,
pp34-56, 1993.
2
Liu DY and Baker HWG. Tests for
human sperm-zona pellucida binding and penetration. In Male Factor in Human Infertility, Tesarik, J. (ed), Ares-Serono
Symposia Publications, Frontiers in Endocrinology, Volume 8. Rome, Italy,
pp169-185, 1994.
3
Bourne H, Richings N, Liu DY,
Clarke GN, Harari O and Baker HGW. Sperm preparation for intracytoplasmic
injection: methods and relationship to fertilization results. In Intracytoplasmic Sperm Injection-The
Revolution in Male Infertility,. Flaherty S. P. and Matthews C.D. (eds),
CSIRO Australia, pp43-49. 1995.
4
Baker HWG and Liu DY. Assessment
of nuclear maturity. In Human
Spermatozoa in Assisted Reproduction (2nd Edition), Acosta, A.A. and
Kruger, T.F. (eds), The Parthenon Publishing Group, New York, pp93-203, 1996.
5
Baker HWG, Liu DY and Bourne H.
Assessment of the male and preparation of sperm for ARTs. In Handbook of In Vitro Fertilization.
(2nd Edition), Trounson A.O. and Gardner D.K., (eds), CRC press, Boca Raton,
pp99-126, 1999.
Symposium Abstracts or Papers
1. Liu
DY and Baker HWG. Hypoosmotic sperm swelling test as a sperm function test
(Letter to the Editor and reply). Fertility
and Sterility 51:365-366, 1989.
2 Pantke
P, Hyland JH, Galloway DB, Liu DY and Baker HWG. Development of zona pellucida
sperm binding assay for the assessment of stallion fertility. Australian Equine Veterinarian,
10(2):91, 1992.
3 Liu
DY and Baker HWG. Relationship between sperm motility assessed with the
Hamilton-Thorn Motility Analyzer and fertilization rates in vitro. Fertility Digest 5:8-12, 1992
4 Liu
DY and Baker HWG. Tests of sperm function. Fertility
and Sterility (Letter to Editor and reply) 59:698-699, 1993
5 Liu
DY and Baker HWG. Morphology of spermatozoa bound to the zona pellucida of
human oocytes that failed to fertilize in vitro. Fertility Digest 3: 3-5, 1993.
6
Baker HWG, Liu DY, Bourne H and
Lopata A. Diagnosis of sperm defects in selecting patients for assisted
fertilization. Fertility Digest 3:
9-11, 1994.
7
Baker HWG and Liu DY and Bourne
H. Clinical characteristics of disordered zona pellucida induced acrosome
reaction: a newly described cause of infertility. In Human Acrosome reaction
(eds) P Fenichel, J Parinaud. Colloque INSERM/John Libbey Eurotext Ltd 1995 Vol
236 pp434-435.
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