孙青原，男，1964年12月4日出生于山东省招远县

 

 

1. 学历及培训经历

1999年10月-2001年8月   University of Missouri-Columbia, USA

博士后(Research   associate)

1996年10月－1998年4月  Bar-Ilan University, Israel博士后

1994年8月－1996年8月   中国科学院动物研究所生殖生物学国家重点实验室博士后

1991年8月－1994年6月   东北农业大学生物工程系博士研究生

1985年7月－1988年6月   东北农业大学生物工程系硕士研究生

1981年9月－1985年6月   山东农业大学畜牧兽医系本科生

 

2. 工作经历

2001年12月-现在          中国科学院动物研究所研究员，博士生导师

中国科学院研究生院教授

生殖生物学国家重点实验室常务副主任, 生殖生物学中心主任

2003年3月-2003年4月    日本National Institute of Agrobiological Sciences， Invited JAPS Fellow

2000年3月-2001年11月   中国科学院动物研究所研究员，博士生导师，受精生物学研究组组长

1998年12月—2000年2月   中国科学院动物研究所研究员，硕士生导师，受精生物学研究组组长

1998年5月－1998年11月  中国科学院动物研究所副研究员，硕士生导师，受精生物学研究组组长

1996年9月－1998年4月   中国科学院动物研究所副研究员

1996年1月－1996年4月   日本National Institute of Animal Industry， STA Fellow

1988年7月－1991年7月   山东农业大学畜牧兽医系助教

 

3. 学术兼职

中国动物学会理事、副秘书长

中国动物学会细胞及分子显微技术分会理事长

中国动物学会生殖生物学分会理事、秘书长

中国生理学会代谢、内分泌及生殖生物学专业委员会委员

中国畜牧兽医学会生物技术分会理事

美国生殖生物学会会员

中国科学院动物研究所学术委员会委员

生殖生物学国家重点实验室学术委员会委员

第19届国际动物学大会科学委员会副主席

Journal of Reproduction and Development编辑委员会委员

《动物学报》编辑委员会委员

《动物学杂志》编辑委员会委员

《生殖医学杂志》编辑委员会委员

美国Society for the Study of Reproduction会员

 

4.主要研究领域

1)卵母细胞减数分裂和受精的信号转导

MEK/MAPK/p90rsr pathway, PKC pathway, ubiquitin-proteasome pathway, meiotic spindle checkpoints, securing/separase/cohesin

2) 早期胚胎发育和动物克隆的的基因表达

DNA methylation/demethylation，histone acelyation/methylation mitochondrial DNA

 

5.杂志Reviewer:

Biology of Reproduction；Reproduction；Developmental Dynamics；Reproduction, Fertility and Development；Microscopy Research and Technique；Developmental and Reproductive Biology；中国科学；科学通报；生物化学与生物物理进展；生物物理与生物化学学报；自然科学进展；动物学报；兽类学报；动物学研究；动物学杂志；生物工程学报；生殖医学；生殖与避孕；细胞生物学杂志；遗传学报；遗传

 

6. 主持过的课题

1）黑龙江省自然科学基金项目：牛卵母细胞超低温冷冻保存研究（1993-1994），1.6万元，已完成。

2）中国博士后科学基金项目：哺乳动物受精机理研究—卵子激发精发育的分子机制（1995-1996），1000美元，已完成。

3）国家自然科学基金项目：哺乳动物受精过程中卵子激发精核发育的研究（1997－1999），8万元，已完成。

4）继任主持“863”项目：精原细胞核替代合子核跨越发育的研究（1996－1998），36万元，已完成。

5）预攀登项目二级课题：受精分子机理（1997－2000），95万元，已完成。

6）中国科学院生物科学和技术研究特别支持项目：卵母细胞减数分裂细胞周期调控点及相关信号通路研究（1998－2000）20万元，已完成。

7）国家教委留学人员基金：MAPK在卵母细胞成熟和受精过程中的作用（1999-2000）,3.5万元,已完成。

8）人事部留学回国人员择优基金：MAPK在调节哺乳动物卵母细胞减数分裂中的作用（1999-2000），2万元,已完成。

9）生殖生物学国家重点实验室基金：MAPK在卵母细胞成熟和受精过程中的作用（1999-2000），4万元,已完成。

10）中国科学院知识创新工程重要方向性课题“动物克隆的基础理论研究”（KSCX2-SW-303,2001-2004），70万元，正在进行。

11）国家重大基础研究（973）项目“人类生殖健康基础研究”中的三级课题：哺乳动物受精的信号转导（G1999055902,2000-2004），145万元，正在进行。

12）国家杰出青年科学基金项目，哺乳动物卵母细胞减数分裂成熟和受精的信号转导，（30225010，2003-2006年），100万元

13）国家自然科学基金面上项目：哺乳动物卵子纺锤体形态与非整倍体胚胎形成的关系 （30170358， 2002-2004），15万元

14）国家自然科学基金面上项目：异种克隆过程中供体细胞核去分化分子机理研究（30371037，2004-2006）24万元

15) 国家自然科学基金重点项目：哺乳动物卵母细胞减数分裂、受精和早期胚胎发育过程中细胞增殖和分化的分子基础（30430530， 2005-2007），140万元

 

7．获奖

1．  中国人民解放军总后勤部科技进步三等奖，1998

2．  中国科学院青年科学家奖，1999

3．  国家杰出青年科学基金资助，2002

4．  中国科学院杰出青年，2003

5．  全国留学回国人员先进个人和国家留学回国人员成就奖，2003

6．  中国青年科技奖，2004

7．  新世纪百千万人才工程国家级人选, 2004

8．  新疆自治区科技进步一等奖， 2003

 

8. Invited talks (after 2003)

Workshop: From Egg to Fertilization, Involvement of MAPK cascade during mammalian oocyte maturation and fertilization, 2003, Kobe University, Japan

 

Invited talk: the regulation of MAPK cascade on oocyte maturation and fertilization, 2003, Tsukuba, National Institute of Agrobiological Sciences,Japan

 

Cross-Strait Symposium on Comparative Endocrinology and Reproductive Biology, The role of MAPK and its regulation during mammalian oocyte maturation and fertilization, Taiwan, China

 

International Young Scientists Forum on Modern Reproductive biology and Biotechnology, Involvement of mitogen-activated protein kinase cascade during oocyte maturation and fertilization in mammals, 2004, Huhhot, Inner Mongolia University, China

 

International Symposium on Reproductive Medicine, oocyte growth and maturation,2004， Jinan, Shandong University, China

 

Symposium and Workshop of in vitro Maturatioin (IVM) of Human Oocytes, Oocyte growth and maturation, 2004，Nanjing, Nanjing Medical University, China,

 

The First Russian-Chinese Symposium of Young Scientists “The Science about Life”, Regulation of oocyte maturation and fertilization by MAPK cascade, 2004，Pushchino, Rusian Academy of Sciences, Russia,

 

2004 KRIBB conference: oocyte maturation workshop, MAPK cascade regulation on pig oocyte maturation and fertilization, 2004, Daejeon, The Korea Research Institute of Bioscience and Biotechnology, Korea

 

The 4th International Symposium of Developmental Biology, The role of MAPK cascade and its regulation during mammalian oocyte maturation and fertilization, Suwan, 2004,Korea

 

9. 论著目录 (*通讯作者)

Full Papers Published in Peer-Reviewed Journals (*correspondence)

1.     Zhong ZS, Huo LJ, Liang CG, Chen DY, Schatten H, Sun QY* (2005) Small GTPase RhoA is Required for Ooplasmic Segregation, Spindle Rotation, but not for Spindle Organization and Chromosome Separation during Mouse Oocyte Maturation, Fertilization, and Early Cleavage. Mol Reprod Dev (in press).

2.    Chen T, Zhang YL, Jiang Y, Liu SZ, Schatten H, Chen DY, Sun QY* (2004) DNA methylation pattern The DNA methylation events in normal and cloned rabbit embryos. BEBS letters (In press).

3.    Zheng ZY, Li QZ, Chen DY, Schatten H, Sun QY* (2004) Translocation of Phospho-PKCs Implies Their Roles in Meiotic Spindle Organization, Polar Body Emission and Nuclear Activity in Mouse Eggs. Reproduction (in press)

4.   Zhang D, Li M, Ma W, Hou Y, Li YH, Li SW, Sun QY, Wang WH. Localization of Mitotic Arrest Deficient 1 (MAD1) in Mouse Oocytes During the First Meiosis and Its Functions as a Spindle Checkpoint Protein. Biol Reprod. 2004 Sep 1 [Epub ahead of print]

5.   Wei Ma, Dong Zhang, Yi Hou, Yong-Hai Li, Qing-Yuan Sun, Xiao-Fang Sun, and Wei-Hua Wang （2004）Reduced expression of MAD2, Bcl-2, and MAP kinase activity in pig oocytes after in vitro aging are associated with defects in sister chromatid segregation during meiosis II and embryo fragmentation after activation. Biol Reprod published 6 October 2004; 10.1095/biolreprod.104.030999

6.     Zhang LS，Zhang KY, Yao LJ, Liu SZ,Yang CX, Zhong ZS, Zheng YL, Sun QY, Chen DY （2004）Somatice cell nuclear remodeling in Rassir oocyte cytoplasm. Zygote 12：179-184.

7.     Liu SZ, Zhou ZM, Chen T, Zhang YL, Wen DC, Kou ZH, Li ZD, Sun QY, Chen DY （2004） Blastocysts produced by nuclear transfer between chicken blastodermal cells and rabbit oocytes. Mol Reprod Dev 69:296-302.

8.  Jiang Y, Liu SZ, Zhang YL, Jian MX, Sun QY, Chen DY (2004) The fate of mitochondria in ibex-hirus reconstructed early embryos. 生物物理与生物化学学报 36:371-374.

9. Huo LJ, Fan HY, Zhong ZS, Chen DY, Schatten H, Sun QY* (2004) Ubiquitin-Proteasome Pathway modulates Modulates Mouse Oocyte Meiotic Maturation and Fertilization via Regulation of MAPK Cascade and Cyclin B1 Degradation. Mechanisms of Development 121:1275-1287.

10. Dong Zhang, Wei Ma, Yong-Hai Li, Yi Hou, Shi-Wen Li, Xiao-Qian Meng, Xiao-Fang Sun, Qing-Yuan Sun, and Wei-Hua Wang (2004)Intraoocyte Localization of MAD2 and its relationship with kinetochores, microtubules, and chromosomes in rat oocytes during meiosis. Biol Reprod 71:740-748.

11. Zheng YL, Jang MX, Zhang YL, Sun QY, Chen DY (2004) Effects of oocyte age, cumulus cells and injection methods on in vitro development of intracytoplasmic sperm injection rabbit embryos. Zygote 12:75-80.

12. Li M, Li YH, Hou Y, Sun XF, Sun QY, Wang WH (2004) Isolation and culture of pluripotent cells from in vitro produced porcine embryos. Zygote 12:43-48.

13.Meng XQ, Fan HY, Zhong ZS, Zhang G,Li YL, Chen DY, Sun QY*.(2004)Loclaization of g-tubulin in mouse eggs during meiotic maturation, fertilization and early embryonic development. J Reprod Dev 50:97-105.

14.Huo LJ, Fan HY, Liang CG,Yu LZ, Zhong ZS,Chen Dy, Sun QY* (2004)Regulation of ubiquitin-proteasome pathway on pig oocyte meiotic maturation and fertilization. Biology of Reproduction 71:835-862.

15.Yao LJ, zhang LS, Kou ZH, Chen DY, Sun QY* (2004) Translocation of g-tubulin during porcine oocyte maturation and activation. Prog. Biochem Biophys 31:339-344.

16.Huo LJ， Fan Hy， Chen DY， Sun QY* (2004).Function and interaction of maturation-promoting factor and mitogen-activated protein kinase during meiotic maturation and fertilization of oocyte. Progress in Natural Science  14:562-567.

17.Li M, Hou Y, Sun XF, Sun QY, Wang WH.(2004) Improved isolation and culture of ES cells from Chinese miniature pigs. J Reprod Dev 50:237-244.

18.Yao LJ, Zhong ZS, Chen DY, Schatten H, Sun QY* (2004). Auroa-A is a critical regulator of microtubule organization and nuclear activity in pig oocytes, fertilized eggs and early embryos. Biology of Reproduction 70(5)1392-1399.

19.Sun QY, Fuchimato D,Nagai T.(2004) Regulatory roles of ubiquitin-proteasome pathway in pig oocyte meiotic maturation and fertilization. Theriogenology 62：245-255.

20.Fan HY, huo LJ, Chen DY, Schatten H, Sun QY* (2004)Protein kinase C and mitogen-actuivated protein kinase cascade in mouse cumulus cells:crosstalk and effect on meiotic resumption of oocytes. Biology of Reproduction 70:1178-1187.

21.Fan HY，Huo LJ， Sun QY* （2004）Methodology for Detection of spindle-accociated proteins by confocal microscopy in mammalian oocytes. Prog. Biochem Biophys 31：54-58

22.Fan HY，Sun QY* (2004)Involvement of MAPK cascade during oocyte maturation and fertilization in mammals. Biology of Reproduction* 70:535-547.

23.Quan HM, Meng XQ, Hou Y, Sun QY* (2004) Sperm penetration of immature and maturing oocytes does not affect phosphorylation of mitogen-activated protein kinase in pigs. Reprod Fertil Dev 15:383-387.

24.Quan HM, Fen HY, Meng XQ, Huo LJ, Chen DY, Scahtten H, Sun QY* (2003) Effect of protein kinase C activation on mouse oocyte meiotic maturation, fertilization and early embryo development. Zygote 11:329-337.

25.Liu RH, Sun QY, Li YH, Jiao LH, Wang WH (2003) Maturation of porcine oocytes after cooling at the germinal vesicle stage. Zygote 11:299-305.

26.Fan HY Huo LJ, Meng XQ, Zhong ZS, Hou Y, Chen DY, Sun QY* (2003)Involvement of calcium/calmodulin-dependent protein kinase II (CaMKII) in meiotic maturation and activation of pig oocytes. Biology of Reproduction 69:1552-1564.

27.Yao LJ, Fan HY, Tong C, Chen DY, Schatten H, and Sun QY* (2003)Polo-like Kinase-1 in Porcine Oocyte Meiotic Maturation, Fertilization and Early Embryonic Mitosis. Cell Mol Biol 49：399-405.

28.Wen DC， Yang CX， Cheng Y， Li JS， Liu ZH， Sun QY， Zhang JX， Lei L， Kou ZH， Chen DY （2003）Compariusion of developmental capacity for intra- and interspecies cloned cat （Felis catus） embryos. Molecular Reproduction and Development 66：38-45.

29.Wa W,Hou Y, Sun QY, Sun XF, Wang WH (2003)Localization of centrosome proteins and their association with chromosomes and microtubules during meiotic maturation of pig oocytes. Reproduction 126:731-738.

30.Sun QY，Nagai T.2003.Molecular mechanisms underlying pig oocyte maturation and fertilization. J Repro Dev 49:347-359.

31.Li YH, Hou Yi, Ma W, Yuan JX, Zhang D, Sun QY, Wang WH.(2004) Localization of CD9 in pig oocytes and its effects on sperm-egg interaction. Reproduction 127:151-157.

32.毕春明，文端成，徐营，孙青原，陈大元（2003）小鼠囊胚内细胞团置换。科学通报48：1064-1068。

33.Yang CX, Kou ZH, Wang K, Jiang Y, Mao WW, Sun QY, Sheng HZ, Chen DY. (2004) Quantitative analysis of mitochondiral DNAs in macaque embryos reprogrammed by rabbit oocytes. Reproduction 127:201-205.

34.Cai-Xia Yang, Zhi-Ming Han， Duan-Cheng Wen, Qing-Yuan Sun, Ke-Ying Zhang, Li-Sheng Zhang , Yu-Qi Wu, Zhao-Hui Kou, Hui-Zhen Sheng, Da-Yuan Chen （2003）In Vitro Development and Mitochondria Fate of Macaque-Rabbit Cloned Embryos. Molecular Reproduction and Development 65:396-401.

35.毕春明，李劲松，吴昱琪，张立生，寇朝辉，姜岩，蒋满喜，自力，孙青原，陈大元 （2003）牛卵母细胞不同时间的成熟培养可提高核移植效率。自然科学进展 13：997-1001。

36．Tong C, Fan HY, Li SW, Chen DY, Song XF, Schatten H, Sun QY* (2003) Effect of MEK inhibitor U0126 on meiotic progression in mouse oocytes: microtubule organization, asymmetric division and metaphase II arrest. Cell Research 13:375-383.

37．Fan HY, Huo LJ, Sun QY* (2003) Roles of calmodulin-dependent protein kinase II in meiotic maturation and fertilization of oocytes. Prog. Biochem Biophys 30:171-174.

38．Zhi-Ming Han, Da-Yuan Chen, Jin-Song Li, Qing-Yuan Sun, Peng-Yan Wang, Jun Du, He-Min Zhang （2003）Flow cytometric cell-cycle analysis of cultured fibroblast cells from the giant panda, Ailuropoda melanoleuca L. Cell Biology International 27:349-353.

39．Chen DY, Li JS, Han ZM, Lai L, Liu ZH, Kou ZH, Ma SY, Du QK, Sun QY (2003) somatic cell bovine cloning: Effect of donor cell and recipients. Chinese Science Bulletin 48:549-554.陈大元，李劲松，韩之明，雷蕾，刘忠华，寇朝辉，马世援，杜启科，孙青原.（2003）我国首次独立自主的成年牛耳体细胞克隆牛研究 科学通报 48:767-771.

40．Fan HY, Tong C, Chen DY, Sun QY* (2003) Roles of protein kinase C in oocyte meiotic maturation and fertilization. Progress in Natural Science 13:402-406.范衡宇，佟超, 陈大元 孙青原*（2003）蛋白激酶C在卵母细胞减数分裂成熟及受精中的作用。自然科学进展 13：478-484。

41．Cheng Y， Fan HY，Wen DC， tong C， Zhu ZY， Lei L， Sun QY， Chen DY （2003） Asynchronous cytoplast and karyoplast transplantation in mouse oocytes。Molecular Reproduction and Development 65:278-22

42.Fan HY, Tong C, Teng CB, Lian L, Li SW, Yang ZM, Chen DY, Schatten H, Sun QY*. (2003)Characterization of polo-like kinase-1 in rat oocytes and early embryos implies its functional roles in the regulation of meiotic maturation, fertilization and cleavage. Molecular Reproduction and Development 65:318-329.

43.Zhu ZY, Chen DY, Li JS, Lian L, Lei L, Han ZM, Sun QY (2003) Rotation of MII spindle is controlled by microfilaments in mouse oocytes. Biology of Reproduction 68:934-937.

44.Fan HY, Tong C, Lian L, Li SW, Gao WX, Cheng Y, Chen DY, Schatten H, Sun QY* (2003) Characterization of ribosomal protein kinase p90rsk during meiotic maturation and fertilization in pig oocytes: mitogen-activated protein kinase-associated activation and localization. Biology of Reproduction 68:968-977.

45.Liu RU, Sun QY, Li YH, Jiao LH, Wang WH  （2003）Effects of cooling on oocyte maturation, meiotic spindle structure and chromosome alignment in in vitro maturing porcine oocytes. Molecular Reproduction and Development 65:212-218.

46. Cheng Y, Han ZM, Sun QY, Chen DY (2003) Development of mouse embryos derived from oocytes reconstructed by metaphase I spindle transfer. Zygote 11:53-59.

47. Sun QY. (2003) Cellular and molecular mechanisms leading to cortical reaction and polyspermy block in mammalian eggs. Microscopy Research Techniques 61:342-348.

48．Yu HQ, Chen DY, Shorgan B, Sun QY* (2002) Phosphorylation of MAP kinase and p90rsk and its regulation during in vitro maturation of cumulus-enclosed rabbit oocytes. Zygote 10:311-316.

49.Fan HY, Li MY, Tong C, Chen DY, Xiao GL, Sun QY* (2002) Inhibitory effects of cAMP and protein kinase C on meiotic maturation and MAP kinase phosphorylation in porcine oocytes. Molecular Reproduction and Development 63:480-487.

50．Fan HY, Tong C, Li SW, Chen DY, Sun QY* (2002) Microtubule organization during in vitro development of rat oocytes. Prog. Biochem Biophys 29:708-713.

51.范衡宇，佟超, 孙青原* （2002）核糖体S6蛋白激酶p90rsk与卵母细胞减数分裂。生物化学与生物物理进展29:506-509。

52.Sun QY, Wu GM, Lai L, Bonk A, Cabot R, Park K, Day BN,Prather RS, Schatten H. (2002) Regulation of mitogen-activated protein kinase phosphorylation, chromatin behavior, microtubule organization, and cell cycle progression by protein phosphatases during pig oocyte maturation and fertilization in vitro. Biology of Reproduction 66:580-588.

53.Lu Q, Smith G, Chen DY, Han ZM, Sun QY*.(2002) Activation of protein kinse C induces MAP kinase dephosphorylation and pronucleus formation in rat oocytes. Biology of Reproduction 67:64-69.

54.Fan HY, Tong C, Lian L, Chen DY, Schatten H, Sun QY* (2002) Translocation of classical protein kinase C (cPKC) isoforms in porcine oocytes: implications of PKC involvement in the regulation of nuclear activity and cortical granule exocytosis. Experimental Cell Research 277:183-191.

55.Tong C, Fan HY, Lian L, Chen DY, Schatten H, Sun QY*.(2002) Polo-like kinase is a pivotal regulator of microtubule organization during mouse oocyte maturation, fertilization and early embryonic mitosis. Biology of Reproduction 67:546-554.

56.Cheong HC, Park KW, Im GS, Lai L, Sun QY, Day BN, Perather RS (2002) Effect of elevated Ca2+ concentration in fusion/activation medium on the fusion and development of porcine fetal fibroblast nuclear transfer embryos. Molecular Reproduction and Development 61:488-492.

57.Li JS, Chen DY, Han ZM, Zhu ZY, Wen DC, Sun QY, Liu ZH, Wang MK, Lian L, Du J, Wang PY, Zhang HM (2002) Serial nuclear transfer improves the development of interspecies constructed giant panda embryos. Chinese Science Bulletin 47:467-469.

58. Li MY, Fan Hy, Tong C, Chen Dy, Xia GL, Su