|
Asian Journal of Andrology (2009) 11: 176-182. doi: 10.1038/aja.2008.33; published online 5 January 2009.
Tumor formation of prostate cancer cells influenced by stromal cells from the transitional or peripheral zones of the normal prostate
Fu-Jun Zhao1, Bang-Min Han1, Sheng-Qiang Yu1 and Shu-Jie Xia1
1 Department of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
Correspondence: Prof. Shu-Jie Xia, Department of Urology, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China. Fax:+86-21-63241377 E-mail: xsjurologist@163.com
Received 17 October 2008; Accepted 22 October 2008; Published online 5 January 2009.
| Abstract |
This study was designed to investigate the different involvements of prostatic stromal cells from the normal transitional zone (TZ) or peripheral zone (PZ) in the carcinogenesis of prostate cancer (PCa) epithelial cells (PC-3) in vitro and in vivo co-culture models. Ultra-structures and gene expression profiles of primary cultures of human prostatic stromal cells from the normal TZ or PZ were analyzed by electron microscopy and microarray analysis. In vitro and in vivo co-culture models composed of normal TZ or PZ stromal cells and human PCa PC-3 cells were established. We assessed tumor growth and weight in the in vivo nude mice model. There are morphological and ultra-structural differences in stromal cells from TZ and PZ of the normal prostate. In all, 514 differentially expressed genes were selected by microarray analysis; 483 genes were more highly expressed in stromal cells from TZ and 31 were more highly expressed in those from PZ. Co-culture with PZ stromal cells and transforming growth factor-β1 (TGF-β1) increased the tumor growth of PC-3 cells in vitro and in vivo, as well as Bcl-2 expression. On the other hand, stromal cells of TZ suppressed PC-3 cell tumor growth in the mouse model. We conclude that ultra-structures and gene expression differ between the stromal cells from TZ or PZ of the normal prostate, and stroma–epithelium interactions from TZ or PZ might be responsible for the distinct zonal localization of prostate tumor formation.
Keywords: co-culture, epithelial cells, stromal cells, transitional zone, peripheral zone, prostate cancer |
|
