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Asian Journal of Andrology (2010) 12: 431-441. doi: 10.1038/aja.2009.92; published online 22 February 2010.
Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa
Juan Carlos Martinez-Soto1,2, Juan de DiosHourcade3, Alfonso Gutiérrez-Adán3, José Lorenzo Landeras2 and Joaquín Gadea1
1 Department of Physiology, School of Veterinary, Murcia University, Murcia 30100, Spain
2 IVI- MURCIA, Murcia 30007, Spain
3 Department of Animal Reproduction, INIA, Madrid 28040, Spain
Correspondence: Dr Joaquín Gadea, Department of Physiology, School of Veterinary, Murcia University, Murcia 30100, Spain. Fax: +34-868-884-147 E-mail: jgadea@um.es
Received 13 July 2009; Revised 21 September 2009; Accepted 24 December 2009; Published online 22 February 2010.
| Abstract |
In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 μmol L−1) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozen-thawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.
Keywords: antioxidants; cryopreservation; DNA damage; genistein; isoflavones; sperm motility |
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