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- Original Article -
Differential impact of aging and gender on lipid and
lipoprotein profiles in a cohort of healthy Chinese Singaporeans
Victor H. H. Goh1,2, Terry Y. Y.
Tong1, Helen P. P. Mok1, Baharudin
Said1
1Department of Obstetrics and Gynecology, National University of Singapore, National University Hospital, Kent Ridge
119074, Singapore
2Core Lab at General Clinical Research Center, LA Biomed, Harbor-UCLA Medical Center and
Department of Medicine, Division of Endocrinology, David Geffen School of
Medicine at UCLA, 1124W Carson Street, Torrance, CA
90502, USA
Abstract
Aim: To evaluate the impact of age and gender on lipid and lipoprotein profiles and the burden of dyslipidemia in a
cohort of healthy Chinese Singaporean.
Methods: A total of 1 775 healthy Chinese, 536 men and 1 239 women aged
between 30 and 70 years old were involved in the present study.
Results: Gender differences in all lipid and
lipoprotein levels were clearly evident. Singaporean Chinese men have significantly higher levels of total cholesterol (TC),
triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and total cholesterol/high density
lipoprotein-cholesterol (TC/HDL-C), and lower levels of HDL-C than women. Although lipid and lipoprotein levels in men did not
change in the different age groups, those in women, especially TC, LDL-C and TC/HDL-C, were significantly higher
in older women (> 50 years old) than corresponding levels in younger women (30_46 years old). Furthermore, TG
was significantly correlated with lipids and lipoproteins differently in men and women. If 100 mg/dL of LDL-C were
to be adopted as the therapeutic cut-off level, then the burden of care will be huge as approximately 90% of both
Chinese men and women have LDL-C greater than 100 mg/dL.
Conclusion: In light of the findings of the present
study, we suggest that preventive measures to promote the reduction in risk of coronary heart disease (CHD) must
address the high proportion of men and women with high LDL-C, and that these measures should take into account
both the gender and age factors. For men, reduction of high cholesterol must start early in life, whereas for women,
steps must be taken earlier to mitigate the anticipated sharp increase in risk, especially after menopause.
(Asian J Androl 2007 Nov; 9: 787_794)
Keywords: total cholesterol; low density lipoprotein-cholesterol; high density lipoprotein-cholesterol; triglyceride; total cholesterol/high
density lipoprotein-cholesterol; cardiovascular diseases, artheriosclerosis; Asian men and women
Correspondence to: Prof. Victor H. H. Goh, Core Lab at General Clinical Research Center, LA Biomed, Harbor-UCLA Medical Center and
Department of Medicine, Division of Endocrinology, David Geffen School of Medicine at UCLA, 1124W Carson Street, Torrance, CA
90502, USA.
Tel: +1-310-2221-855 Fax: +1-310-5330-627
E-mail: vgoh@labiomed.org
Received 2006-09-25 Accepted 2007-02-08
DOI: 10.1111/j.1745-7262.2007.00294.x
1 Introduction
As Asian countries continue to develop and progress
economically, the incidence of lifestyle diseases, such as
coronary heart disease (CHD), arteriosclerosis and
metabolic syndrome, is rising. To minimize the incidence of
these lifestyle diseases, there is a need to identify and
address the risk factors early [1]. Today, there is
evidence to show that the risks of CHD and arteriosclerosis
increase with a decline in high density
lipoprotein-cholesterol (HDL-C), increase in low density
lipoprotein-cholesterol (LDL-C), triglyceride (TG) and total
cholesterol (TC) [2]. The National Cholesterol Education
Program Adult Treatment Panel III [3] identifies LDL-C as
the primary target for cholesterol-lowering therapies. The
therapeutic target is the reduction of LDL-C to levels
below 100 mg/dL. In addition, strategies to increase
HDL-C through regular exercise and to lower TG levels
through proper diet and cutting down smoking and
alcohol consumption are equally important lifestyle
modifications that will help to mitigate the risk of CHD and
arteriosclerosis as a result of dyslipidemia [3].
There is a perception that Asian diets, unlike those in
developed countries, are low in fat and, hence, Asians
are less likely to have high cholesterol than their
deve-loped-world counterparts. This is certainly not the case.
Geographical and socioeconomic factors and diet all
influence lipid and lipoprotein profiles. On top of these
factors, age and gender might impact the profile of lipids
and lipoproteins differently. Therefore, we evaluated the
impact of age and gender on lipid and lipoprotein profiles
of a cohort of generally healthy Chinese Singaporeans.
We also assessed the burden of dyslipidemia and the
considered challenges in tackling the increasing risks of CHD
and arteriosclerosis in Asia.
2 Methods
2.1 Subjects
Institutional approval for the study was obtained and
each volunteer gave written consent to participate. A
total of 1 775 healthy subjects, 536 men and 1 239 women
aged between 30 and 70 years old, were included in this
analysis. Subjects were recruited from the general
public through an open invitation to participate. All subjects
included in this analysis were ethnic Chinese. They
represented a wide spectrum in the society with educational
background ranging from those with primary to those
with tertiary educations. Subjects' vocations ranged from
non-working to clerical, technical and professional
positions. Salary scales ranged from < SG$1 000/month
to > SG$10 000/month. Singapore is a city-country, its
population is strictly urban, and there is no rural
population. Certain major illnesses, such as diabetes and
hypogonadism affect lipid and lipoprotein profiles. To
assess the impact of age and gender on lipid and
lipoprotein profiles, only subjects with no known existing or
history of major medical illnesses, such as cancer,
hypertension, thyroid dysfunction, diabetes, osteoporotic
fracture and cardiovascular events were recruited into
the present study.
Each subject answered a detailed questionnaire with
questions on medical, dietary, social, sex, and family
history, and other relevant history regarding
consumption of hormones or food supplements.
2.2 Serum lipid and triglyceride levels
An overnight 12-h fasting blood sample was collected
and serum levels of TC and TG were measured using an
automated procedure. HDL-C was determined after
precipitation of apolipoprotein B with sodium
phosphotungstate and MgCl2 [4]. LDL-C was computed
according to the following formula: LDL-C = TC _ (HDL-C +
[TG × 0.45]). According to this formula, the calculated
LDL-C values are not valid if TG levels are high. The
ratio of TC to HDL-C (TC/HDL-C) was used as the atherogenic index [5].
2.3 Statistical analysis
Statistical analyses were performed using SPSS for
windows version 12 (SPSS Inc., Chicago, IL, USA).
One-way analysis of variance, χ2, Fisher exact, unpaired
t-test and Pearson linear regression test were used where
appropriate.
3 Results
Gender differences in lipid and lipoprotein levels were
clearly evident. Overall, Singaporean Chinese men have
significantly higher levels of TC, TG, LDL-C and
TC/HDL-C and lower levels of HDL-C than corresponding
levels in women (Figure 1).
The gender difference in lipid and lipoprotein levels
was further affected by age. In men, HDL-C level was
positively correlated with age (r = 0.141,
P = 0.001), whereas TC/HDL-C was negatively correlated with age
(r = _0.11, P = 0.011). In women, however, TC
(r = 0.284,
P < 10_4), HDL-C
(r = 0.063, P = 0.023),
LDL-C (r = 0.218,
P < 10-4) and TC/HDL-C
(r = 0.143, P =0.000) were all positively correlated with age. A
gender difference in the relationship among TG levels and
other lipid and lipoprotein levels was also noted. TG
was significantly correlated to TC (r = 0.283,
P < 10-4) in men, but not in women. In addition, the degrees of
negative correlation of TG with HDL-C (men,
r = _0.351,
P < 10_4; women,
r = _0.095, P = 0.001) and positive
correlation with TC/HDL-C (men,
r = 0.582,
P < 10-4; women,
r = 0.107,
P < 10-4) were much higher in men
than in women.
Although TC and LDL-C levels in men were not affected by age, those in women showed progressive and
significant increases. Both TC and LDL-C levels increased
in age groups of women from 30 to 50 years, and
thereafter the concentrations did not change. The levels of
TC in the age groups between 30 to 45 years in men
were significantly higher than corresponding levels in
women, whereas those of LDL-C in the age groups between 30 and 50 years in men were significantly higher
than corresponding levels in women (Figures 2 and 3).
Interestingly, in the 61_65 year age group, the gender
differences for TC and LDL-C seen in the younger age
groups were reversed: both TC and LDL-C levels were
significantly higher in women than corresponding levels
in men (Figures 2 and 3).
A clear gender difference in HDL-C levels was
also noted: levels in women in age groups from 30 to
65 years were significantly higher than corresponding levels in
men. However, HDL-C levels in men and women in the
66_70 year age group were not significantly different (Figure 4).
Triglyceride levels in men and women were not
affected by age. The TG levels in men were significantly
higher than corresponding levels in women for age groups
from 36 to 60 years, and thereafter, no gender
difference in levels was noted (Figure 5).
The gender differences, with higher TC and lower
HDL-C in men, respectively, than in women, have accentuated the gender differences in TC/HDL-C ratios
(Figure 6). The ratio remained significantly higher in
men than in women up to 60-year age. In the higher
age groups, however, a moderation of the TC/HDL-C ratio was noted in men. The TC/HDL-C ratio in women
in the 61_65-year age group was significantly higher
than corresponding levels in the 30_35-year age group.
In men, a reduction in the TC/HDL-C levels was noted
in the 61_65-year age group when compared to the
41_45-year age group (Figure 6).
Although the effects of gender and age on the lipid
and lipoprotein profiles are clearly seen in Figures 1_6,
the burden of dyslipidemia is best assessed by tabulating
the various lipid levels according to the
recommendations of the National Cholesterol Education Program Adult
Treatment Panel III [2].
Although the proportions of men and women with TC
above 200 mg/dL were not significantly different, there
were significantly more men with high TC
(> 240 mg/dL) than women
(χ2-test, P = 0.005, Table 1). In addition,
although the distribution of men (32%) with high TC in
the age groups between 30 and 70 years was not
age-dependent, those in women were. Significantly more
women in the older age groups (above 50 years) have
high TC compared to women in the younger age groups
(Table 1).
Approximately 90% of normal healthy men and women in our population have LDL-C greater than
100 mg/dL, the therapeutic target level of the NECP
ATP III (Table 2). Significantly more men than women
have high LDL-C (> 130 mg/dL and > 160 mg/dL)
(Table 2). The proportions of men with LDL-C greater
than 160 mg/dL were not significantly different among
the various age groups. However, more older women
(> 50 years old) have high LDL-C (> 160 mg/dL) than
younger women (< 50 years old) (Table 2).
4 Discussion
Our data showed that being male is one of the most
important determinants of lipids and lipoproteins as risk
factors for CHD and arteriosclerosis. Singaporean
Chinese men have higher risks arising from TC, LDL-C,
HDL-C, TC/HDL-C and TG, than Chinese women. In general, although age was not a determinant for lipids
and lipoproteins in Singaporean Chinese men, it was an
important determinant in Singaporean Chinese women.
Except for HDL-C, all other lipid and lipoprotein levels
(TC, LDL-C, TC/HDL-C and TG) were significantly higher in older women, especially those over 55 years,
when compared to younger women in their thirties.
Therefore, being of the male sex was disadvantageous
with respect to all the lipid and lipoprotein risk factors.
However, menopause in woman was an important risk factor. These observations reflect similar trends shown
in other populations, including those in developed
countries [6].
The data also showed a gender difference in the
metabolism of triglyceride in Singaporean Chinese men and
women. In men, higher triglyceride levels were
associated with higher TC and TC/HDL-C and lower
HDL-C levels. This relationship was not as acute in women.
This observation implies that the high carbohydrates in
the Asian diet is reflected in high triglyceride, which, in
turn, is associated with high TC and TC/HDL-C and lower
HDL-C levels in men.
Coronary heart disease is becoming a serious health
problem as Singapore becomes more affluent [7, 8]. In
2000, it was the second leading cause of death in Singapore, accounting for 24.5% of all deaths [9]. The
increasing trend of CHD is not confined to Singapore,
but also to countries in Asia that have become more
affluent.
To mitigate risks of CHD and arteriosclerosis in the
huge population bases in Asia, preventive measures must
be formulated and applied to the general public. In other
words, a public health approach must be adopted [10].
The results shown in the current study imply that any
management modality to address dyslipidemia must take
into account gender and age factors. We suggest that
strategies to reduce TC, LDL-C, TG and to increase
HDL-C levels should be instituted as early as possible.
However, for men, the urgency to start early is more
acute as high TC, LDL-C and TG levels were noted here
as early as 30 years, whereas in women, they tend to
occur in the menopausal years.
Studies have shown that lowering LDL-C and TC levels can mitigate cardiovascular risks [11]. To reduce
the burden of coronary atherosclerosis, the National
Cholesterol Education Program Adult Treatment Panel
III (NCEP-ATP III) recommends that the concentrations
of LDL-C and other CHD risk factors be maintained at
their optimal levels [3]. Results from the present study
showed the extent of the problem of dyslipidemia in our
general population and clarify the challenges ahead if the
NCEP's strategy of lowering the lipid and lipoprotein
risk factors for CHD is to be adopted. Although the
NCEP-ATP III recommendation is for those with CHD
or with high risks of developing CHD, it is not
unrealistic to use the same recommendation as a target for a
preventive management strategy. According to the lipid
and lipoprotein profiles shown for Singaporean Chinese
men and women, the magnitude of this task varies
according to the indices and cut-off target levels one uses
as well as whether the target group is men or women.
To bring total cholesterol below the upper limit of
normal (200 mg/dL), the targeted groups include 71.6% of
men and 68.7% of women. However, if one were to use
the TC/HDL-C of < 4.5, then fewer men (50.0%) and
especially women (15.5%) would be targeted.
The NCEP ATP_III's recommendation of lowering LDL-C is a key strategic approach to reducing the risk
of CHD. In Singapore, the current cut-off value for
LDL-C remains at 130 mg/dL. Even if this cut-off is
used, almost equal proportions of apparently healthy men
(66.4%) and women (53.4%) would be targeted for
LDL-C lowering modalities. However, if, as recommended by the NCEP ATP III, LDL-C was lowered
below 100 mg/dL in our local population, then approximately
90% of both men and women in the general population
would have to be targeted. This represents a huge
burden of care. It is, however, for the government and the
medical fraternity to decide which cut-off level to adopt
as the target for any preventive strategy.
Triglyceride is an independent risk factor for CHD
[12], and significantly more men (14.9%) than women
(4.4%) should be targeted for inclusion in a TG lowering
strategy to help to reduce the risk of CHD in our local
population.
Most men (87.1%) and women (97.1%), regardless of age in our cohort, have HDL-C levels > 39 mg/dL.
However, significantly more women (53.8%) than men
(17.7%) have levels of HDL-C higher than 62 mg/dL.
Hence, more men than women need to increase their HDL-C levels to benefit from its cardio-protective effect.
Various preventive measures must be adopted to
reduce the high lipids and lipoproteins among the Chinese
Singaporean men and women. Among these, public
educational and advocacy strategies must be adopted for
long-term effectiveness. The key to success is to educate
the general public and to get individuals to take
ownership of their own health. Past experience has shown
that soft public awareness programs do not seem to work
effectively. More aggressive and sustained educational
and promotional programs, involving the health
promotion authority together with family physicians as well as
civic interest groups, will, more likely, be effective.
For those with lower levels of LDL-C, between 100 mg/dL and 130 mg/dL, low HDL-C and high TG
levels, advocacy for lifestyle modifications, including
proper diet and adequate exercise, would be the first
line of action. Lifestyle and diet significantly
influence the lipid risk factor levels of Singaporean
Chinese [13]. However, for subjects with higher levels
of LDL-C (> 160 mg/dL) or when lifestyle changes are
not effective, the use of various types of statins that have
been shown to be effective should be considered [14].
Dyslipidemia and obesity are risk factors for
coronary arteriosclerosis [15, 16]. Therefore, strategies for
lowering LDL-C and TG as well as increasing HDL-C [17] to mitigate their effect on CHD must also be carried
out in conjunction with the drive to curb the increasing
trend of obesity in the population. As shown in an earlier
study, the incidences of obesity in Singaporean men and
women were 7% and 13.9%, respectively [18]. These
incidences are somewhat higher than those reported for
Taiwanese Chinese men (3.2%) and women (6.4%), but
are lower than incidences in more developed countries
[19]. Therefore, strategies for reduction of obesity
should be intimately tied to those for reduction of bad
cholesterol.
In the light of the current findings, preventive
measures to promote the reduction in risk of CHD must
address the high proportion of men and women with dyslipidemia, and any measure adopted should take into
account gender and age factors. For men, reduction of
high cholesterol must start early in life, whereas for
women, the higher risk after menopause must be considered. In all, promotion of a healthy lifestyle,
including proper and appropriate diet, adequate exercise,
especially among women and men in the middle age group, is critical in the battle to reduce the
cardiovascular risk arising from dyslipidemia and high triglyceride
levels.
Acknowledgment
We would like to thank staff in the Endocrine Laboratory, Department of Obstetrics and Gynaecology,
National University of Singapore for their invaluable
assistance in carrying out the study. This project was
supported by funds given by the National University of
Singapore, Academic Research Fund (R-174-000-067-112).
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