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Volume 12, Issue 2 (March 2010) 12, 263–270; 10.1038/aja.2009.67
Optimal dose of busulfan for depleting testicular germ cells of recipient mice before spermatogonial transplantation
De-Zhi Wang1, Xin-Hua Zhou1, Yu-Lin Yuan1 and Xin-Min Zheng2
1 Department of Anatomy and Embryology, Wuhan University School of Medicine, Wuhan 430071, China
2 Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Correspondence: Prof. Xin-Min Zheng, zxm_whu@sina.com
Received 10 July 2009; Revised 6 August 2009; Accepted 10 September 2009; Published online 14 December 2009.
| Abstract |
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Successful spermatogonial transplantation requires depletion of the host germ cells to allow efficient colonization of the donor spermatogonial stem cells. Although a sterilizing drug, busulfan (Myleran), is commonly used for preparing a recipient mouse before transplantation, the optimal dose of this drug has not yet been defined. The present study investigated the effects of different doses of busulfan (10–50 mg per kg body weight) on survival rate, testicular mass and histomorphology, and on the haploid spermatids and spermatozoa of male BALB/c mice. The results suggest that a dosage of 30 mg kg−1 is optimal for the ablative treatment with busulfan used to prepare the recipient mice. This dose results in an adequate depletion of the host germ cells for colonization of donor-derived spermatogonial stem cells and causes the lowest death rate of the animals.
Keywords: busulfan; germ cells; infertility; mice; myleran; spermatogonial transplantation; testis
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