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Volume 12, Issue 2 (March 2010) 12, 278–280; 10.1038/aja.2009.93
Mountant-induced loss of Shorr-stained blue sperm tails
Barbara Hellenkemper1, Anda Ströse1,2, Trevor G. Cooper1
1 Centre of Reproductive Medicine and Andrology of the University Münster D-48149, Germany
2 Department of Life Science and Engineering, Saxion Hogescholen, Enschede 7500, Netherlands
Correspondence: Dr Trevor G. Cooper,tgcooper@gmx.de
Received 13 December 2009; Revised 29 December 2009; Accepted 31 December 2009; Published online 25 January 2010.
| Abstract |
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It has been reported that Shorr staining provides additional morphological information on the motility of spermatozoa in semen, by distinguishing between red and blue flagella. With our routine methods (involving mounting slides) we were unable to confirm these observations. The presence of both red- and blue-coloured sperm tails in Shorr-stained semen smears was apparent, however, if slides were unmounted. Only a very weak association between blue flagellar staining and immotility was observed. Stating whether a mountant was used should be reported.
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