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Abstract

Volume 14, Issue 4 (July 2012) 14, 566–573; 10.1038/aja.2012.30

Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation

Dong-Sheng Hou1,2,*, Wen-Min Long3,*, Jian Shen1,2, Li-Ping Zhao3, Xiao-Yan Pang3 and Chen Xu1,2

1 Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
2 Shanghai Key Laboratory of Reproductive Medicine, Shanghai 200025, China
3 National Key Laboratory of Microbial Metabolism, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200025, China

* These authors contributed equally to this work.

Correspondence: Dr C Xu, (chenx1955@126.com); Dr XY Pang, (xypang@sjtu.edu.cn)

Received 15 December 2011; Revised 6 February 2012; Accepted 29 February 2012

Abstract

The expressed prostatic secretions (EPSs) of men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), infertile men and normal men were subjected to microbiological study. EPSs were collected from the subjects, which included 26 normal men, 11 infertile patients and 51 CP/CPPS patients. DNA was extracted from each specimen, and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers. The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men, but without any difference among the three patient groups. The denaturing gradient gel electrophoresis (DGGE) method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues. Principal component analysis (PCA) and partial least squares (PLS) analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups. Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients (P<0.05). Two bands were identified as priority factors in the discrepancy of category IIIA and category IIIB prostatitis patients (P<0.05). According to this research, the ecological balance of the prostate and low urethra tract, when considered as a microenvironment, might play an important role in the maintenance of a healthy male reproductive tract.

Keywords: bacteria; chronic prostatitis/chronic pelvic pain syndrome; denaturing gradient gel electrophoresis; prostatitis

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