Home  |   Archive  |   Online Submission  |   News & Events  |   Subscribe  |   APFA  |   Society  |   Contact Us  |   中文版

Ahead of print
Authors' Accepted
Current Issue
Special Issues
Browse by Category

Manuscript Submission

Online Submission
Online Review
Instruction for Authors
Instruction for Reviewers
English Corner new!

About AJA

About AJA
Editorial Board
Contact Us

Resources & Services

Email alert

Download area

Copyright licence
EndNote style file
Manuscript word template
Guidance for AJA figures
    preparation (in English)

Guidance for AJA figures
    preparation (in Chinese)

Proof-reading for the

AJA Club (in English)
AJA Club (in Chinese)


Volume 15, Issue 4 (July 2013) 15, 461–465; 10.1038/aja.2013.22

Analysis of circulating regulatory T cells (CD4+CD25+CD127) after cryosurgery in prostate cancer

Tong-Guo Si1,*, Jun-Ping Wang2,* and Zhi Guo1

1 Department of Interventional Therapy, Tianjin Cancer Hospital, Tianjin Medical University, Tianjin 300060, China
2 Department of Radiology, Tianjin Medical University General Hospital, Tianjin 300052, China

* These authors contributed equally to this work.

Correspondence: Dr Z Guo, (drguozhi@163.com)

Received 21 July 2012; Revised 27 October 2012; Accepted 2 February 2013


This study was performed to assess the response of regulatory T cells (Tregs) following cryosurgery in prostate cancer (PCa) patients by measuring their frequency and immune function. Blood was collected prior to and at 4 and 8 weeks after treatment in 30 patients with high-risk PCa who underwent cryosurgery and from 15 healthy volunteers. Circulating CD4+CD25+CD127 Tregs were isolated. Their frequency was detected by flow cytometry, and immune suppressive function was evaluated by measuring the proliferation of CD4+CD25- T cells cocultured with Tregs. The results showed that the percentage of circulating CD4+CD25+CD127 Tregs was increased in PCa patients compared to healthy volunteers (7.6%±0.73% vs. 5.8%±0.54%, P<0.001). The frequency of circulating CD4+CD25+CD127 Tregs was reduced 4 weeks after cryosurgery compared to before surgery (6.3%±0.58% vs. 7.6%±0.73%, P<0.001), and the decrease persisted for 8 weeks. However, the suppressive function of Tregs was increased in eight of 12 patients, which might contribute to cancer recurrence. Then the response of circulating Tregs is complicated after cryosurgery for PCa, and further studies are warranted.

Keywords:cryosurgery; immune response; prostate cancer (PCa); regulatory T cell (Tregs)

PDF | PDF | 中文摘要 |

Browse:  3265
Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.