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Volume 15, Issue 4 (July 2013) 15, 539–544; 10.1038/aja.2013.43

Role of LM23 in cell proliferation and apoptosis and its expression during the testis development

Qing Liu1,*, Ya-Juan Song1,2,*, Li-Jun Meng3, Fen Hu1, Li-Xia Gou1, Chang-Hong Jia1, Hong-Mei Tang1, Wei-Jie Wang1, Mi Li1, Xiu-Jun Zhang1 and Meng-Chun Jia4

1 College of Life Sciences, Hebei United University, Tangshan 063000, China
2 Department of Clinical Laboratory, Handan Central Hospital, Handan 056002, China
3 Department of Environment & Chemical Engineering, Tangshan College, Tangshan 063000, China
4 Department of Reproductive Endocrinology, National Research Institute for Family Planning, Beijing 100081, China

*These authors contributed equally to this work.

Correspondence: Dr XJ Zhang, (zhangxiujun66@163.com); Dr MC Jia, (mengchunjia@163.com)

Received 10 January 2013; Revised 20 February 2013; Accepted 17 March 2013; Advance online publication 20 May 2013


LM23, a gene expressed specifically in the testis in a stage-specific manner, has a diverse range of functions that are important in both the life and death of spermatogenic cells. The aim of this study was to further investigate the expression of LM23 in the developing rat testis and the biological function of LM23 in proliferation and antiapoptosis in vitro. Semiquantitative reverse transcription (RT)-PCR and real-time PCR were used to examine the expression of LM23 in testis at different developmental stages. The results suggested that LM23 mRNA levels in the testis increased progressively after birth. The role of LM23 in proliferation was analyzed with cell counting kit-8 (CCK8), colony-forming efficiency (CFE) and flow cytometry assays. The results indicated that ectopic expression of LM23 in 293T cells significantly promoted cell proliferation by increasing cell numbers in S phase. Several methods were used, including CCK8, annexin V and propidium iodide staining and western blotting, to determine the role of LM23 in apoptosis. The results showed that LM23 played a protective role in H2O2-induced apoptosis of 293T cells, mediated at least in part through the Akt/PI3K signal pathway. Taken together, these results provide new insights into the role of LM23 in the development of the testes and spermatogenesis.

Keywords: apoptosis; LM23; proliferation; rat testis

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.