Volume 15, Issue 6 (November 2013) 15, 764–769; 10.1038/aja.2013.59
Nerve growth factor signaling following unilateral pelvic ganglionectomy in the rat ventral prostate is age dependent
Carol A Podlasek1, Rudrani Ghosh1, Omer Onur Cakir1, Christopher Bond1, Kevin E McKenna2 and Kevin T McVary3
1Department of Urology, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA 2Department of Physiology, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA 3Division of Urology, Southern Illinois University School of Medicine, Springfield, IL 62794, USA Corresponde
Correspondence: Dr C Podlasek, (cap325@northwestern.edu)
Received 21 December 2012; Revised 12 March 2013; Accepted 20 April 2013 Advance online publication 22 July 2013
Abstract |
Benign prostatic hyperplasia (BPH) is a serious health concern and is an underlying cause of lower urinary tract symptoms (LUTS) in many men. In affected men, LUTS/BPH is believed to result from benign proliferation of the prostate resulting in bladder outlet obstruction. Postnatal growth of the prostate is controlled via growth factor and endocrine mechanisms. However, little attention had been given to the function of the autonomic nervous system in prostate growth and differentiation. Nerve growth factor (NGF) is a prostatic mitogen that has a trophic role in autonomic sensory end organ interaction. In this study, we examine how the autonomic nervous system influences prostate growth as a function of age by quantifying NGF in the rat ventral prostate (VP) after pelvic ganglionectomy. Unilateral pelvic ganglionectomy was performed on postnatal days 30 (P30), 60 and 120 Sprague–Dawley rats in comparison to sham controls (n=39). Semiquantitative RT-PCR, Western blotting and immunohistochemical analysis for NGF were performed on denervated, intact (contralateral side) and sham control VP 7 days after surgery. Ngf RNA expression was significantly increased in the denervated and intact hyperplastic VP. Western blotting showed age-dependent increases in NGF protein at P60 in the contralateral intact VP. NGF was localized in the nerves, basal cells and columnar epithelium of the prostatic ducts. Denervation causes age-dependent increases in NGF in the VP, which is a potential mechanism by which the autonomic nervous system may regulate prostate growth and lead to BPH/LUTS.
Keywords: denervation; nerve growth factor; prostate growth; ventral prostate
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