|
Volume 7, Issue 1 (January 2005) 7, 33–37; 10.1111/j.1745-7262.2005.00017.x
Glutathione peroxidase activity in cell cultures from different regions of human epididymis
Enrique Castellón1, Hernán Rioseco1, Juan Rojas1, Michel Royer1, Eduardo Salas1, Héctor Contreras1 and Christian Huidobro2
1 Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago 6530499, Chile
2 Urology Department, Clinic Hospital J. J. Aguirre, University of Chile, Santiago 6530499, Chile
Correspondence: Enrique A. Castelln, Ph.D., Physiology and Biophysics Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, P.O. Box 70005, Santiago 6530499, Chile. Tel: +56-2-678-6863, Fax: +56-2-777-6916 E-mail: ecastell@med.uchile.cl
Received: 2004-02-11 Accepted: 2004-10-18
| Abstract |
|
Aim: To study the secretory activity and androgen regulation of glutathione peroxidase (GPx) in epithelial cell cultures from human epididymis.
Methods: Tissue was obtained from patients undergoing therapeutic orchidectomy for prostatic cancer. Epithelial cell cultures were obtained from the caput, corpus and cauda epididymides. Enzymatic activity was measured in conditioned media by colorimetric methods in absence or presence of 1, 10 or 100 nmolL-1 testosterone. The effect of 1 molL-1 flutamide was also evaluated.
Results: GPx activity was higher in cultures from corpus and cauda than caput epididymidis. The presence of different concentrations of testosterone increase enzyme activity in cell cultures from all epididymal regions. Addition of flutamide reverses the androgen dependent increase of GPx activity.
Conclusion: GPx activity is secreted from human epididymal cells in a region dependent manner and is regulated by androgens.
Keywords:glutathione peroxidase, human epididymis, cell culture, androgen regulation
Full Text |
PDF |
|
| |
| Browse: 3005 |
|
