|
Volume 8, Issue 2 (March 2006) 8, 183–187; 10.1111/j.1745-7262.2006.00121.x
DAZ1/DAZ2 cluster deletion mediated by gr/gr recombination per se may not be sufficient for spermatogenesis impairment: a study of Chinese normozoospermic men
Yuan Yang, Cui-Ying Xiao, Zhou-Cun A, Si-Zhong Zhang, Xiang Li and Si-Xiao Zhang
1.Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China
2.Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China
3.Division of Human Morbid Genomics, National Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China
Correspondence: Prof. Si-Zhong Zhang, Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu 610041, China. Fax: +86-28-8516-4009. E-mail: szzhang@mcwcums.com
Received 15 September 2005; Accepted 29 November 2005.
| Abstract |
|
Aim: To explore the possible effect of the deleted in azoospermia (DAZ) copy cluster deletion on spermatogenesis in the Chinese population, the deletion of the azoospermia factor c (AZFc) region was analyzed in 346 normozoospermic men.
Methods: Three DAZ single nucleotide variant loci and seven AZFc-specific sequence-tagged sites were examined with polymerase chain reaction (PCR)-restriction fragment length polymorphism and routine PCR.
Results: Five (1.4%) of the normozoospermic men were found to have deletion of gr/gr-DAZ1/DAZ2. None of the men were found to have b2/b4-entire DAZ deletion.
Conclusion: The presence of gr/gr-DAZ1 /DAZ2 deletion in five men with normozoospermia suggests that this deletion per se may not be sufficient for spermatogenic impairment in Chinese men.
Keywords: azoospermia factor c, deleted in azoospermia, gene deletion, normozoospermia
Full Text |
PDF |
中文摘要 |
|
| |
| Browse: 4193 |
|
