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Volume 8, Issue 4 (July 2006) 8, 405–409; 10.1111/j.1745-7262.2006.00167.x
Effects of tetrandrine on cytosolic free calcium concentration in corpus cavernosum smooth muscle cells of rabbits
Ji-Hong Liu, Jun Chen, Tao Wang, Bo Liu, Jun Yang, Xiao-Wen Chen, Shao-Gang Wang, Chun-Ping Yin and Zhang-Qun Ye
1.Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2.Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Correspondence: Dr Ji-Hong Liu, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. Fax: +86-27-8360-8783. E-mail: jhliu@tjh.tjmu.edu.cn; Dr Jun Chen, Department of Urology, Tongji Hospital,
Received 20 September 2005; Accepted 12 February 2006
| Abstract |
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Aim: To study the relaxation mechanisms of tetrandrine (Tet) on the corpus cavernosum smooth muscle.
Methods: The corpus cavernosum smooth muscle cells from New Zealand white rabbits were cultured in vitro. [Ca2+]i was measured by Fluorescence Ion Digital Imaging System, using Fluo-2/AM as a Ca2+-sensitive fluorescent indicator.
Results: Tet (1, 10 and 100 mol/L) had no effect on the resting [Ca2+]i (P > 0.05). In the presence of extracellular Ca2+ (2.5 mmol/L), Tet (1, 10 and 100 mol/L) inhibited [Ca2+]i elevation induced by high K+ and phenylephrine (PE) in a concentration-dependent manner (P < 0.05). In calcium free solution containing egtaic acid, Tet (1 and 10 mol/L) had no inhibitory effects on [Ca2+]i elevation induced by PE (P > 0.05). However, Tet (100 mol/L) inhibited [Ca2+]i elevation induced by PE (P < 0.05).
Conclusion: Tet inhibited the Ca2+ influx from the extracellular site via voltage-activated Ca2+ channel and 1-adrenoceptor-operated Ca2+ channel. At a high concentration, Tet might inhibit the cytosolic calcium pool release in cultured corpus cavernosum smooth muscle cells. This inhibitory action on [Ca2+]i might be one of the relaxation mechanisms of Tet on the corpus cavernosum smooth muscle.
Keywords: tetrandrine, penis, smooth muscle cell, free calcium, corpus cavernosum, erectile dysfunction
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