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Volume 8, Issue 5 (September 2006) 8, 549–554; 10.1111/j.1745-7262.2006.00196.x
Upregulation of macrophage migration inhibitory factor and calgizzarin by androgen in TM4 mouse Sertoli cell
Hiroyuki Kasumi, Shinji Komori, Kazuko Sakata, Naoko Yamamoto, Tomohiko Yamasaki, Yonehiro Kanemura and Koji Koyama
1.Department of Obstetrics and Gynecology, Hyogo College of Medicine, Nishinomiya 663-8501, Japan
2.Laboratory of Developmental Biology and Reproduction, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Nishinomiya 663-8501, Japan
3.Tissue Engineering Research Center, National Institute of Advanced Industrial Science and Technology, Amagasaki 661-0974, Japan
Correspondence: Dr Shinji Kmori, Department of Obstetrics and Gynecology, Hyogo College of Medicine, 1-1 mukogawa-cho, Nishinomiya 663-8501, Japan. Fax: +81-798-46-4163. E-mail: komor615@hyo-med.ad.jp
Received 9 January 2006; Accepted 12 April 2006
| Abstract |
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Aim: To identify proteins induced by androgen in Sertoli cells during spermatogenesis.
Methods: We analyzed protein profiles in TM4 Sertoli cells treated with dihydrotestosterone (DHT) using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS).
Results: We found increases in the expression of a 5.0-kDa protein at 15 min, an 11.3-kDa protein at 24 h and 4.3 kDa, 5.7 kDa, 5.8 kDa, 9.95 kDa and 9.98 kDa proteins at 48 h after the treatment. In contrast, the expression of 6.3 kDa and 8.6 kDa proteins decreased at 30 min, and 4.9 kDa, 5.0 kDa, 12.4 kDa and 19.8 kDa proteins at 48 h after the treatment. The 11.3-kDa protein was identified as macrophage migration inhibitory factor (MIF) known to having various functions. The 9.98-kDa protein was identified as calgizzarin related to calcium channels. The timing of their expression suggests that MIF and calgizzarin are involved in late regulation of spermatogenesis in Sertoli cells by androgen.
Conclusion: MIF and calgizzarin are two important androgen-responsive proteins produced by Sertoli cells and they might play a role in regulating spermatogenesis.
Keywords: androgen, Sertoli cell, spermatogenesis, surface enhanced laser desorption ionization time-of-flight mass spectrometry, macrophage migration inhibitory factor, calgizzarin
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