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Volume 9, Issue 3 (May 2007) 9, 388–393; 10.1111/j.1745-7262.2007.00168.x
Gossypol inhibits proliferation of endometrioma cells in culture
Shawky Z A Badawy, Abdul-Kader Souid, Violeta Cuenca, Nicholas Montalto and Frances Shue
1.Department of Obstetrics and Gynecology, SUNY Upstate Medical University, Syracuse, New York 13210, USA
2.Department of Pediatrics, SUNY Upstate Medical University, Syracuse, New York 13210, USA
Correspondence: Prof. Shawky Z. A. Badawy, Department of Obstetrics and Gynecology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, New York 13210, USA. Fax: 315-470-2838. E-mail: badawys@upstate.edu
Received 25 October 2005; Accepted 27 February 2006.
| Abstract |
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Aim: To evaluate the anti-proliferative activity and mitochondrial toxicity of gossypol in endometrioma cells maintained in short-term cultures.
Methods: (A) Three endometrioma cell lines from patients were treated with 25 or 50 nmol/L gossypol for up to 12 days. The effect of gossypol on the cell growth was recorded. (B) A phosphorescence oxygen analyzer was used to determine the effects of gossypol on mitochondrial oxygen consumption of six endometrioma cell lines from patients. (C) Cellular gossypol accumulations in three endometrioma cell lines from patients were measured by high-pressure liquid chromatography.
Results: Proliferation of the endometrioma cells was inhibited by 25 and 50 nmol/L gossypol. Respiration of the endometrioma cells was inhibited by 10 mol/L gossypol. Cellular gossypol was detected in the endometrioma cell lines that were treated for 24 h with 10 and 0.3 mol/L gossypol.
Conclusion: Gossypol invokes a potent toxicity on cultured endometrioma cells.
Keywords: gossypol, endometrioma, mitochondria, oxygen consumption, cellular respiration
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