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Volume 9, Issue 6 (November 2007) 9, 801–808; 10.1111/j.1745-7262.2007.00293.x
Novel flutamide regulated genes in the rat ventral prostate: differential modulation of their expression by castration and flutamide treatments
Anil M Limaye, Irfan Asangani, Namrata Bora and Paturu Kondaiah
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India
Correspondence: Dr Paturu Kondaiah, Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore 560012, India. Fax: +91-080-2360-0999. E-mail: paturu@mrdg.iisc.ernet.in
Received 3 November 2006; Accepted 19 April 2007.
| Abstract |
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Aim: To identify flutamide regulated genes in the rat ventral prostate.
Methods: Total RNA from ventral prostates of control and flutamide treated rats were isolated. Differentially expressed transcripts were identified using differential display reverse transcriptase polymerase chain reaction. The effect of castration on the expression of flutamide-regulated transcripts was studied.
Results: We have identified 2-microglobulin, cytoplasmic FMR1 interacting protein 2 and pumilio 1 as flutamide induced and spermine binding protein and ribophorin II as flutamide repressed targets in the rat ventral prostate. Although flutamide treatment caused an induction of pumilio 1 mRNA, castration had no effect.
Conclusion: Castration and flutamide treatments exert differential effects on gene expression. Flutamide might also have direct AR independent effects, which might have implications in the emergence of androgen independent prostate cancer and the failure of flutamide therapy.
Keywords: androgens, antiandrogen, castration, differential display reverse transcriptase polymerase chain reaction, flutamide, prostate
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