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Volume 9, Issue 6 (November 2007) 9, 731–737; 10.1111/j.1745-7262.2007.00311.x
Detecting subtle changes in sperm membranes in veterinary andrology
Fernando J Peña
Department of Animal Health and Medicine Faculty of Veterinary Medicine University of Extremadura, Cáceres 10071, Spain
Correspondence: Dr Fernando J. Peña, Section of Reproduction and Obstetrics, Department of Herd Health and Medicine, Faculty of Veterinary Medicine, Avd de la Universidad s/n, Cáceres 10071, Spain. Fax: +34-927-257-110. E-mail: fjuanpvega@unex.es
Received 8 December 2006; Accepted 16 May 2007.
| Abstract |
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Thanks to the increasing use of flow cytometry in research in veterinary spermatology, many new membrane integrity assays have been developed over the past decade. These assays are important because of their superior ability to forecast fertility when compared with other tests, such as sperm motility. This major component of the sperm quality assessment has generated new investigations with the aim of developing tests that can detect membrane damage in a very early state. Using phospholipid transposition tests, early changes in membrane permeability and fluidity can be assessed in a large number of spermatozoa using fluorescent probes in combination with flow cytometry.
Keywords: sperm membranes, flow cytometry, early membrane changes, veterinary
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