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Volume 10, Issue 2 (March 2008) 10, 243–248; 10.1111/j.1745-7262.2008.00330.x
Effects of 17beta-estradiol on distribution of primordial germ cell migration in male chicks
Xiu-Mei Jin, Yi-Xiang Zhang and Zan-Dong Li
1.College of Biological Sciences, China Agricultural University, Beijing 100094, China
2.School of Life Sciences, Huzhou Teachers College, Huzhou 313000, China
Correspondence: Dr Zan-Dong Li, College of Biological Sciences, China Agricultural University, Yuan-MingYuan West Road 2, Beijing 100094, China. Fax: +86-10-6289-5439. E-mail: lzdws@cau.edu.cn
Received 8 September 2006; Accepted 6 June 2007.
| Abstract |
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Aim: To assess whether exogenous estradiol has any effect on migration of primordial germ cells (PGCs) in the chick.
Methods: Fertilized eggs were treated with 17beta-estradiol (E2) (80 μg/egg) at stage X (day 0 of incubation), stages 8-10 (incubation 30 h) and 13-15 (incubation 55 h). Controls received vehicle (emulsion) only. Changes in PGC number were measured on different days according to developmental stages.
Results: In male right gonads, but not in female left gonads, at stages 28-30 (incubation 132 h) significant decreases in the mean number of PGCs aggregating were observed compared with the controls (P < 0.05) while the total PGC number in the right and left gonads at each stage did not change (P > 0.05).
Conclusion: The present study provides evidence that E2 has significant effects on the localization of PGCs in male right, but not female left, gonads of chicken embryos at stages 28-30, compared with controls.
Keywords: 17beta-estradiol, primordial germ cells, male chick
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