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Volume 10, Issue 4 (July 2008) 10, 561–568; 10.1111/j.1745-7262.2008.00392.x
Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis
Shinichi Hayasaka1, Yukihiro Terada1, Kichiya Suzuki1, Haruo Murakawa2, Ikuo Tachibana2, Tadashi Sankai3, Takashi Murakami1, Nobuo Yaegashi1 and Kunihiro Okamura1
1 Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Miyagi 980-8574, Japan
2 Suzuki Memorial Hospital, Iwanuma, Miyagi 989-2481, Japan
3 Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tukuba, Ibaraki 305-0843, Japan
Correspondence: Yukihiro Terada, M.D., Ph.D., Department of Obstetrics and Gynecology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan. Fax: +81-22-717-7258. E-mail: terada@mail.tains.tohoku.ac.jp
Received 18 September 2007; Accepted 12 January 2008.
| Abstract |
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Aim: To show whether molecular motor dynein on a microtubule track, molecular motor myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein (MyRIP), and vesicle receptor Rab27b on an F-actin track were present during human and monkey spermiogenesis involving intramanchette transport (IMT).
Methods: Spermiogenic cells were obtained from three men with obstructive azoospermia and normal adult cynomolgus monkey (Macaca fascicularis). Immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the proteins were carried out. Samples were analyzed by light microscope.
Results: Using RT-PCR, we found that dynein, myosin Va, MyRIP and Rab27b were expressed in monkey testis. These proteins were localized to the manchette, as shown by immunofluorescence, particularly during human and monkey spermiogenesis.
Conclusion: We speculate that during primate spermiogenesis, those proteins that compose microtubule-based and actin-based vesicle transport systems are actually present in the manchette and might possibly be involved in intramanchette transport.
Keywords: intramanchette transport, manchette, spermiogenesis
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