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Volume 10, Issue 4 (July 2008) 10, 535–541; 10.1111/j.1745-7262.2008.00410.x
Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus
Yan Li1,2, Claudia Lalancette1,2, David Miller3 and Stephen A Krawetz1,2,4
1 Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Wayne 48201, MI, USA
2 Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Wayne 48201, MI, USA
3 Reproduction and Early Development Group, University of Leeds, Institute of Genetics, Health and Therapeutics, Leeds LS29JT, UK
4 Institute for Scientific Computing, Wayne State University, Wayne 48201, MI, USA
Correspondence: Prof. Stephen A Krawetz, 253 C.S. Mott Center; 275 East Hancock; Detroit, MI 48201, USA. Fax: +1-313-577-8554. E-mail: steve@compbio.med.wayne.edu
Received 9 January 2008; Accepted 7 March 2008
| Abstract |
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Aim: To simultaneously determine the localization of histones and protamines within human sperm nuclei.
Methods: Immunofluorescence of the core histones and protamines and fluorescence in situ hybridization of the telomere region of chromosome 16 was assessed in decondensed human sperm nuclei.
Results: Immunofluorescent localization of histones, protamine 1 (PRM1) and protamine 2 (PRM2) along with fluorescence in situ hybridization localization of chromosome 16 telomeric sequences revealed a discrete distribution in sperm nuclei. Histones localized to the posterior ring region (i.e. the sperm nuclear annulus), whereas PRM1 and PRM2 appeared to be dispersed throughout the entire nucleus.
Conclusion: The co-localization of the human core sperm histones with the telomeric regions of chromosome 16 is consistent with the reorganization of specific non-protamine regions into a less compacted state.
Keywords: human sperm nucleus, histone, protamine, telomere
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