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Volume 10, Issue 5 (September 2008) 10, 719–722; 10.1111/j.1745-7262.2008.00419.x
Long-term study of male rabbit urethral mucosa reconstruction using epidermal cell
Qiang Fu1, Chen-Liang Deng2, Xiao-Fei Song1 and Yue-Min Xu1
1 Department of Urology, Shanghai 6th People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
2 Shanghai Tissue Engineering Research and Development Center, Shanghai 200011, China
Correspondence: Dr Qiang Fu, Department of Urology, Shanghai 6th People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China. Fax: +86-21-6386-8709. E-mail: jamesqfu@yahoo.com.cn
Received 21 November 2007; Accepted 22 April 2008
| Abstract |
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Aim: To investigate the transformation of characteristics of epidermal cells from foreskin which were used to reconstruct male rabbit anterior urethra in combination with acellular collagen matrices.
Methods: In three rabbits, autologous foreskin epidermal cells were isolated, expanded in vitro, and seeded (inoculated) onto a tubular acellular collagen matrix, acquired from allogeneic rabbit bladder submucosa. A urethral mucosal defect was created, and urethral reconstruction was performed with the tubular acellular collagen matrix seeded with epidermal cells.
Results: On gross examination at 12 months following the procedure, the mucosa of the urethral grafts appeared lubricous and smooth. Urethrography showed that a wide urethral caliber had been maintained without any sign of strictures. Histological examination showed a transitional cell layer in the graft without evidence of a margin between the graft and the host tissue at 12 months postoperatively.
Conclusion: Epidermal cells seeded onto acellular collagen matrices can be successfully used to reconstruct urethras that have defects and are transformed to transitional epithelial cells.
Keywords: urethral stricture, tissue engineering, foreskin, epidermal
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