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Volume 7, Issue 4 (July 2005) 7, 411–417; 10.1111/j.1745-7262.2005.00056.x
Effects of Basella alba and Hibiscus macranthus extracts on testosterone production of adult rat and bull Ley dig cells
Paul F Moundipa, Nathalie Sara E Beboyl, Fabien Zelefack, Silvère Ngouela, Etienne Tsamo, Wolf-Bernhard Schill and Thomas K Monsees
1.Department of Biochemistry, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon
2.Department of Organic Chemistry, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon
3.Centre for Dermatology and Andrology, Justus Liebig University, Gaffkystr. 1435385 Giessen, Germany
4.Department of Anatomy, University of Technology, Fetscherstr. 74, 01307 Dresden, Germany
Correspondence: Prof. Paul F. Moundipa, Laboratory of Biochemical Toxicology, Department of Biochemistry, University of Yaounde I, P. O. Box 812 Yaounde, Cameroon. Tel: +237-780-4010, Fax: +237-222-1320 E-mail: pmoundipa@uycdc.uninet.cm
Received: 2004-10-19 Accepted: 2005-05-16
| Abstract |
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Aim: To determine the androgenic effects of Basella alba and Hibiscus macranthus extracts in the rat and the bull, and to develop a novel in vitro test system using Leydig cells from bull testes.
Methods: The effect of methanol extracts from both plants on testosterone production in isolated Leydig cells from the rat and the bull was analyzed using 125I-radioimmunoassay (125I-RIA). Rat Leydig cells were obtained by common methods, whereas a novel technique was used to purify Leydig cells from bull testes.
Results: Bull testes from the slaughter house were a cheap source of pure Leydig cells. In culture, these cells produced testosterone for 5–6 days, which can be stimulated by human chorionic gonadotrophin (hCG). Basella alba extracts significantly enhanced testosterone production in bull and rat Leydig cells in a concentration-dependent manner. Hibiscus macranthus showed no androgenic effect but was shown to inhibit testosterone production at higher concentrations.
Conclusion: Leydig cells purified from bull testes can be used as an alternative tool in experimental animal research. Certain fractions of Basella alba extract demonstrated androgenic potential whereas Hibiscus macranthus extracts did not.
Keywords: bull, rats, Leydig cells, testosterone, Basella alba, Hibiscus macranthus, plant extract
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