Home  |  Archive  |  Online Submission  |  News & Events  |  Subscribe  |  APFA  |  Society  |  Links  |  Contact Us  |  中文版
Search   
 
Journal

Ahead of print
Authors' Accepted
    Manuscripts
new!
Current Issue
Archive
Acknowledgments
Special Issues
Browse by Category

Manuscript Submission

Online Submission
Online Review
Instruction for Authors
Instruction for Reviewers
English Corner new!

About AJA

About AJA
Editorial Board
Contact Us
News

Resources & Services

Advertisement
Subscription
Email alert
Proceedings
Reprints

Download area

Copyright licence
EndNote style file
Manuscript word template
Guidance for AJA figures
    preparation (in English)

Guidance for AJA figures
    preparation (in Chinese)

Proof-reading for the
    authors

AJA Club (in English)
AJA Club (in Chinese)

Links

Meetings
Journals
Societies & Institutes
Hospitals
Databases & Libraries
Companies
Websites
Other links

 
Abstract

Volume 7, Issue 4 (July 2005) 7, 369–373; 10.1111/j.1745-7262.2005.00075.x

Erythropoietin gene transfer into rat testes by in vivo electroporation may reduce the risk of germ cell loss caused by cryptorchidism

Masaki Dobashi, Kazumasa Goda, Hiroki Maruyama and Masato Fujisawa

1.Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan
2.Division of Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, Niigata 951-8120, Japan

Received: 2004-12-13 Accepted: 2005-06-23

Abstract

Aim: To investigate the effects of rat Erythropoietin (Epo) on spermatogenesis by transferring rat Epo gene into cryptorchid testes by means of in vivo electroporation.

Methods: Sprague-Dawley rats with surgically-induced unilateral cryptorchidism were divided into three groups: the first group was given intratesticular injections of pCAGGS-Epo (pCAGGS-Epo group), the second group was given intratesticular injections of pCAGGS (pCAGGS group), and the third group were given intratesticular injections of phosphate-buffered saline (PBS group). At the same time, square electric pulses of 30 V were applied six times with a time constant of 100 ms. One or two weeks after injection, each testis was weighed and the ratio of the total number of germ cells to that of Sertoli cells (G/S ratio) was calculated to evaluate the impairment of spermatogenesis. Ten testes taken from each of the three groups were examined at each time point.

Results: The testicular weight after the injection of pCAGGS-Epo or pCAGGS control plasmid was (0.85±0.08) g and (0.83±0.03) g, respectively, at week 1 (P = 0.788) and (0.62±0.06) g and (0.52± 0.02) g, respectively, at week 2 (P = 0.047). At week 1, spermatids and sperm were more abundant in testes with pCAGGS-Epo than those in the control testes. At week 2, spermatids and sperm were hardly detected in either group. The G/S ratio was 23.27±6.80 vs. 18.63± 5.30 at week 1 (P = 0.0078) and 7.16±3.06 vs. 6.05±1.58 at week 2 (P = 0.1471), respectively.

Conclusion: The transfer of Epo to rat testes by in vivo electroporation may reduce the risk of the germ cell loss caused by cryptorchidism.

Keywords: electroporation, gene transfer techniques, erythropoietin, spermatogenesis, Epo

Full Text | PDF |

 
Browse:  864
 
Copyright 1999-2016  Shanghai Materia Medica, Shanghai Jiao Tong University.  All rights reserved