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Volume 8, Issue 1 (January 2006) 8, 61–67; 10.1111/j.1745-7262.2006.00069.x
A differentially methylated region of the DAZ1 gene in spermatic and somatic cells
Zuo-Xiang Li, Xu Ma and Zhao-Hui Wang
1.Department of Genetics, Peking Union Medical College, Beijing 100730 China
2.Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China
Correspondence: Dr Xu Ma, Department of Genetics, Peking Union Medical College, Beijing 100730 China. Fax: +86-10-6217-9059. E-mail: genetic@263.net.cn
Received 3 March 2005; Accepted 6 July 2005.
| Abstract |
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Aim: To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in different cell types.
Methods: Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing.
Results: The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm.
Conclusion: A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.
Keywords: DAZ1 gene, DNA methylation, CpG island, methylated region, spermatogenesis
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