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Volume 9, Issue 5 (September 2007) 9, 679–683; 10.1111/j.1745-7262.2007.00272.x
Gene expression changes of urokinase plasminogen activator and urokinase receptor in rat testes at postnatal stages
Dong-Hui Huang, Hu Zhao, Yong-Hong Tian, Hong-Gang Li, Xiao-Fang Ding and Cheng-Liang Xiong
1.Family Planning Research Institute, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 43003, China
2Department of Human Anatomy, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 43003, China
Correspondence: Dr Cheng-Liang Xiong, Family Planning Research Institute, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China. Fax: +86-27-8369-2605. E-mail: clxiong@mails.tjmu.edu.cn
Received 2 September 2006; Accepted 8 February 2007.
| Abstract |
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Aim: To investigate the gene expression changes of urokinase plasminogen activator (uPA)/urokinase receptor (uPAR) in rat testes at postnatal stages and explore the effects of uPA/uPAR system on the rat spermatogenesis.
Methods: The mRNAs of uPA and uPAR in rat testes were measured by using real-time quantitative polymerase chain reaction (PCR) at postnatal days 0, 5, 10, 15, 21, 28, 35, 42, 49 and 56, respectively.
Results: The tendencies of uPA and uPAR mRNA expression were similar at most postnatal stages except for D0. The expression of uPAR mRNA in rats testes was relatively higher than that of uPA at postnatal D0, and both were decreased until D21, increased obviously at postnatal D28, reached a peak at postnatal D35, then declined sharply at postnatal D42 and retained at a low level afterwards.
Conclusion: The uPA/uPAR system may be strongly linked to spermiation and spermatogenesis via regulating germ cell migration and proliferation, as well as promoting the spermiation and detached residual bodies from the mature spermatids.
Keywords: rats, spermatogenesis, urokinase plasminogen activator, urokinase receptor, quantitative polymerase chain reaction
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