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Volume 21, Issue 2 (March 2019) 21, 190–195; 10.4103/aja.aja_80_18
Direct modification of spermatogonial stem cells using lentivirus vectors in vivo leads to efficient generation of transgenic rats
Bang-Jin Kim1, Yong-Hee Kim1, Myeong-Geun Oh1, Ki-Jung Kim1, Sang-Eun Jung1, Ju-Hee Jin1, Sun-Uk Kim2,3, Kwan-Sik Min4, Buom-Yong Ryu1
1 Department of Animal Science and Technology, College of Biotechnology and Natural Resources, Chung-Ang University, Anseong, Gyeonggi-do 17546, Korea
2 National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungcheongbuk-do 28116, Korea
3 Futuristic Animal Resource and Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Chungcheongbuk-do 28116, Korea
4 Animal Biotechnology, Graduate School of Future Convergence Technology, Department of Animal Life Science, Institute of Genetic Engineering, Hankyong National University, Anseong, Gyeonggi-do 17579, Korea.
5 Present address of Bang-Jin Kim: Department of Cancer Biology, University of Pennsylvania, Philadelphia, PA 19104, USA
Correspondence: Dr. BY Ryu (byryu@cau.ac.kr)
Date of Submission 03-Jan-2018 Date of Acceptance 05-Aug-2018 Date of Web Publication 12-Oct-2018
| Abstract |
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Spermatogonial stem cells (SSCs) transmit genetic information to the next progeny in males. Thus, SSCs are a potential target for germline modifications to generate transgenic animals. In this study, we report a technique for the generation of transgenic rats by in vivo manipulation of SSCs with a high success rate. SSCs in juvenile rats were transduced in vivo with high titers of lentivirus harboring enhanced green fluorescent protein and mated with wild-type females to create founder rats. These founder rats expressed the transgene and passed on the transgene with an overall success rate of 50.0%. Subsequent generations of progeny from the founder rats both expressed and passed on the transgene. Thus, direct modification of SSCs in juvenile rats is an effective means of generating transgenic rats through the male germline. This technology could be adapted to larger animals, in which existing methods for gene modification are inadequate or inapplicable, resulting in the generation of transgenic animals in a variety of species.
Keywords: germline modification; lentivirus; spermatogonial stem cell; testis; transgenic animal
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