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Volume 24, Issue 6 (November 2022) 24, 563–569; 10.4103/aja20229
Update on techniques for cryopreservation of human spermatozoa
Chuan Huang1,2, Yu-Lin Tang1, Jian-Ling Hu3, Wen-Jun Zhou1, Zeng-Hui Huang1,2, Xue-Feng Luo1, Zheng Li3, Wen-Bing Zhu1,2
1 Institute of Reproductive and Stem Cell Engineering, Basic Medicine College, Central South University, Changsha 410000, China
2 Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha 410000, China
3 Department of Andrology, Center for Men's Health, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai 200025, China
Correspondence: Dr. Z Li (lizhengboshi@sjtu.edu.cn) or Dr. WB Zhu (zhuwenbing0971@sina.com)
Date of Submission 27-Aug-2021 Date of Acceptance 09-Feb-2022 Date of Web Publication 01-Apr-2022
| Abstract |
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In the 1960s, sperm cryopreservation was developed as a method to preserve fertility. Currently, techniques for the cryopreservation of human spermatozoa have been widely used in assisted reproduction. However, although sperm cryobiology has made notable achievements, the optimal method for the recovery of viable spermatozoa after cryopreservation remains elusive. Postthawing sperm quality can be affected by cryoprotectants, ice formation, storage conditions, and osmotic stress during the freezing process. This review discusses recent advances in different cryopreservation techniques, cryoprotectants, and freezing and thawing methods during cryopreservation and new indications for the use of cryopreserved spermatozoa.
Keywords: cryodamage; cryoprotectant; dry preservation; freezing and thawing; sperm cryopreservation
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