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Volume 19, Issue 6 (November 2017) 19, 647–654; 10.4103/1008-682X.183379

Melatonin ameliorates the adverse effects of leptin on sperm

Fayez A Almabhouh1 , Khairul Osman2 , Siti Fatimah Ibrahim2 , Sergey Gupalo1 , Justin Gnanou3 , Effendi Ibrahim1 , Harbindar Jeet Singh1, 4, 5

1 Faculty of Medicine, Universiti Teknologi MARA, Sg Buloh Campus, 47000 Sg Buloh, Selangor, Malaysia
2 Faculty of Allied Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda, Kuala Lumpur, Malaysia
3 Faculty of Medicine and Defence Health, National Defence University of Malaysia, Kem Sungai Besi, 57000, Selangor, Malaysia
4 IMMB, Faculty of Medicine, Universiti Teknologi MARA, Sg Buloh Campus, 47000 Sg Buloh, Selangor, Malaysia
5 I-PPerForM, Universiti Teknologi MARA, Sg Buloh Campus, Selangor,Malaysia

Correspondence: Dr. HJ Singh (hjsingh@salam.uitm.edu.my)

Date of Submission 15-Oct-2015 Date of Decision 29-Dec-2015 Date of Acceptance 13-May-2016 Date of Web Publication 14-Oct-2016


This study examined the effects of melatonin on leptin-induced changes in sperm parameters in adult rats. Five groups of Sprague-Dawley rats were treated with either leptin or leptin and melatonin or melatonin for 6 weeks. Leptin was given daily via the intraperitoneal route (60 μg kg−1 body weight) and melatonin was given in drinking water (10 mg kg−1 or 20 mg kg−1 body weight per day). Upon completion, sperm count, sperm morphology, 8-hydroxy-2-deoxyguanosine, Comet assay, TUNEL assay, gene expression profiles of antioxidant enzymes, respiratory chain reaction enzymes, DNA damage, and apoptosis genes were estimated. Data were analyzed using ANOVA. Sperm count was significantly lower whereas the fraction of sperm with abnormal morphology, the level of 8-hydroxy-2-deoxyguanosine, and sperm DNA fragmentation were significantly higher in rats treated with leptin only. Microarray analysis revealed significant upregulation of apoptosis-inducing factor, histone acetyl transferase, respiratory chain reaction enzyme, cell necrosis and DNA repair genes, and downregulation of antioxidant enzyme genes in leptin-treated rats. Real-time polymerase chain reaction showed significant decreases in glutathione peroxidase 1 expression with increases in the expression of apoptosis-inducing factor and histone acetyl transferase in leptin-treated rats. There was no change in the gene expression of caspase-3 (CASP-3). In conclusion, the adverse effects of leptin on sperm can be prevented by concurrent melatonin administration.

Keywords: 8-hydroxy-2-deoxyguanosine; DNA fragmentation; leptin; melatonin; sperm

Keywords: 8-hydroxy-2-deoxyguanosine; DNA fragmentation; leptin; melatonin; sperm

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