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Volume 21, Issue 4 (July 2019) 21, 345–350; 10.4103/aja.aja_65_18

Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice

Cong Shen1,2, Jun Yu2,3, Xi Zhang2, Chen-Chen Liu2, Yue-Shuai Guo2,4, Jia-Wei Zhu1, Ke Zhang1, Yi Yu1, Ting-Ting Gao2,5, Shen-Min Yang1, Hong Li1, Bo Zheng1,2, Xiao-Yan Huang2

1 Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215002, China
2 Department of Histology and Embryology, State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China
3 Department of Obstetrics and Gynecology, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang 212013, China
4 The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University, Wuxi 214002, China
5 Center of Clinical Reproductive Medicine, The Affiliated Changzhou Matemity and Child Health Care Hospital of Nanjing Medical University, Changzhou 213000, China

Correspondence: Dr. B Zheng (mansnoopy@163.com) or Dr. XY Huang (bbhxy@njmu.edu.cn)



While it is known that spermatogonial stem cells (SSCs) initiate the production of male germ cells, the mechanisms of SSC self-renewal, proliferation, and differentiation remain poorly understood. We have previously identified Strawberry Notch 1 (SBNO1), a vertebrate strawberry notch family protein, in the proteome profile for mouse SSC maturation and differentiation, revealing SBNO1 is associated with neonatal testicular development. To explore further the location and function of SBNO1 in the testes, we performed Sbno1 gene knockdown in mice to study the effects of SBNO1 on neonatal testicular and SSC development. Our results revealed that SBNO1 is required for neonatal testicular and SSC development in mice. Particularly, in vitro Sbno1 gene knockdown with morpholino oligonucleotides caused a reduction of SSCs and inactivation of the noncanonical Wnt pathway, through Jun N-terminal kinases. Our study suggests SBNO1 maintains SSCs by promoting the noncanonical Wnt pathway.

Keywords: noncanonical Wnt pathway; spermatogonial stem cells; Strawberry Notch 1 (SBNO1)

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