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Abstract

Volume 20, Issue 1 (January 2018) 20, 24–29; 10.4103/aja.aja_8_17

Aldosterone induces inflammatory cytokines in penile corpus cavernosum by activating the NF-κB pathway

Fei Wu, Zu-Quan Xiong, Shan-Hua Mao, Ji-Meng Hu, Jian-Qing Wang, Hao-Wen Jiang, Qiang Ding

Department of Urology, Fudan Institute of Urology, Huashan Hospital, Fudan University, Shanghai 200040, China

Correspondence: Dr. Q Ding (dr_dingqiang@hotmail.com)

Date of Submission 21-Nov-2016 Date of Decision 09-Jan-2017 Date of Acceptance 06-Feb-2017 Date of Web Publication 04-Apr-2017

Abstract

Emerging evidence indicates that aldosterone and mineralocorticoid receptors (MRs) are associated with the pathogenesis of erectile dysfunction. However, the molecular mechanisms remain largely unknown. In this study, freshly isolated penile corpus cavernosum tissue from rats was treated with aldosterone, with or without MRs inhibitors. Nuclear factor (NF)-kappa B (NF-κB) activity was evaluated by real-time quantitative PCR, luciferase assay, and immunoblot. The results demonstrated that mRNA levels of the NF-κB target genes, including inhibitor of NF-κB alpha (IκB-α), NF-κB1, tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6), were higher after aldosterone treatment. Accordingly, phosphorylation of p65/RelA, IκB-α, and inhibitor of NF-κB kinase-β was markedly increased by aldosterone. Furthermore, knockdown of MRs prevented activation of the NF-κB canonical pathway by aldosterone. Consistent with this finding, ectopic overexpression of MRs enhanced the transcriptional activation of NF-κB by aldosterone. More importantly, the MRs antagonist, spironolactone blocked aldosterone-mediated activation of the canonical NF-κB pathway. In conclusion, aldosterone has an inflammatory effect in the corpus cavernosum penis, inducing NF-κB activation via an MRs-dependent pathway, which may be prevented by selective MRs antagonists. These data reveal the possible role of aldosterone in erectile dysfunction as well as its potential as a novel pharmacologic target for treatment.

Keywords: aldosterone; erectile dysfunction; NF-κB; receptors

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