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Volume 19, Issue 1 (January 2017) 19, 43–46; DOI:10.4103/1008-682X.171570

Comparison of intraperitoneal and intratesticular ozone therapy for the treatment of testicular ischemia-reperfusion injury in rats

Fatih Mete, Huseyin Tarhan, Orcun Celik, Ilker Akarken, Kamil Vural, Rahmi Gokhan Ekin, Isil Aydemir, Yusuf Ozlem Ilbey

1Kanuni Sultan Suleyman Education and Research Hospital, Pediatric Clinic, 34150 Istanbul, Turkey; 2Tepecik Education and Research Hospital, Urology Clinic, 35170
Izmir, Turkey; 3Celal Bayar University, Faculty of Medicine, Department of Pharmacology, 45180 Manisa, Turkey; 4Celal Bayar University, Faculty of Medicine, Department
of Histology and Embryology, 45180 Manisa, Turkey.

Correspondence: Dr. O Celik (orcuncelik82@hotmail.com)

Date of Submission 24-Mar-2015 Date of Decision 24-Jun-2015 Date of Acceptance 30-Oct-2015 Date of Web Publication 05-Jan-2016


We compare the efficacy of intratesticular ozone therapy with intraperitoneal ozone therapy in an experimental rat model. For this
purpose, 24 rats were divided into four groups including sham‑operated, torsion/detorsion, torsion/detorsion plus intraperitoneal
ozone (O‑IP), and torsion/detorsion plus intratesticular ozone (O‑IT). The O‑IP ozone group received a 4 mg kg−1 intraperitoneal
injection of ozone, and the O‑IT group received the same injection epididymally. At 4 h after detorsion, the rats were sacrificed and
orchiectomy materials were assessed histopathologically. Spermatogenesis in the seminiferous tubules and damage to the Sertoli
cells were histopathologically evaluated in the testes using the Johnsen scoring system. i‑NOS and e‑NOS activities in the testis
tissue were also evaluated. Torsion‑detorsion caused a decreased Johnsen score and increased apoptosis of spermatogonial and
Sertoli cells. Ozone injection prevented increases in Johnsen score and i‑NOS level. e‑NOS level of the O‑IP group was significantly
lower than that of the O‑IP group, and i‑NOS level of the O‑IT group was significantly lower than that of the O‑IP group. Local ozone
therapy is more effective than systemic ozone therapy at improving IRI‑related testicular torsion. Our study is the first to show that
the efficacy of intratesticular implementation of ozone therapy is higher than that of intraperitoneal ozone therapy.

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