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Volume 4, Issue 4 (December 2002) 4, 265–268;
PCR analysis of Yq microdeletions in infertile males, a study from South India
S. Ramesh Babu, M. Swarna, P. Padmavathi, P.P. Reddy
Institute of Genetics & Hospital for Genetic Diseases, Scientist, Osmania University, Begumpet, Hyderabad-500 016, India
Advance online publication 1 December 2002
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Aim: To estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India. Methods: Polymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc. Results: Of the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome. Conclusion: The frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome
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