Home  |  Archive  |  Online Submission  |  News & Events  |  Subscribe  |  APFA  |  Society  |  Links  |  Contact Us  |  中文版
Search
 
Journal

Ahead of print
Authors' Accepted
    Manuscripts
new!
Current Issue
Archive
Acknowledgments
Special Issues
Browse by Category

Manuscript Submission

Online Submission
Online Review
Instruction for Authors
Instruction for Reviewers
English Corner new!

About AJA

About AJA
Editorial Board
Contact Us
News

Resources & Services

Advertisement
Subscription
Email alert
Proceedings
Reprints

Download area

Copyright licence
EndNote style file
Manuscript word template
Guidance for AJA figures
    preparation (in English)

Guidance for AJA figures
    preparation (in Chinese)

Proof-reading for the
    authors

AJA Club (in English)
AJA Club (in Chinese)

Links

Meetings
Journals
Societies & Institutes
Hospitals
Databases & Libraries
Companies
Websites
Other links

 
Abstract

Volume 1, Issue 4 (December 1999) 1, 191–194;

Studies on LH modulated 8 kDa peptide involved regulation of testosterone production in rat Leydig cells

P. Ramaraj, A.J. Rao

Department of Biochemistry & Department of Molecular Reproduction, Development & Genetics, Indian Institute of Science, Bangalore-560 0112, India

Advance online publication 1 December 1999

Abstract

Aim: To demonstrate the role of the 8 kDa peptide in regulation of testosterone production by rat Leydig cells. Methods: A peptide similar to 8 kDa peptide purified from immature rat Leydig cells was isolated and purified from rat lung cytosol. Immunological and structural similarity between the peptides purified from lung and Leydig cells was established by Western blot and tryptic map comparison respectively. Results: Addition of the 8 kDa peptide 10, 50, 100, and 150 g decreased the production of testosterone in Leydig cells dose-dependently. But the addition of the peptide 150 g along with hCG had no effect on hCG-stimulated increase in testosterone production. Conclusion: In vitro addition of the peptide purified from lung cytosol to adult rat Leydig cells resulted in a concentration-dependent decrease in basal testosterone production although it had no effect on hCG-stimulated testosterone production.

Full Text |

 
Browse:  200
Copyright 1999-2014    Shanghai Materia Medica, Shanghai Jiao Tong University.    All rights reserved